A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection
Chronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by biolu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1561732/full |
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| author | Michele Tavecchio Silvia Fanni Xuemin Wu Ganna Petruk Manoj Puthia Artur Schmidtchen Artur Schmidtchen |
| author_facet | Michele Tavecchio Silvia Fanni Xuemin Wu Ganna Petruk Manoj Puthia Artur Schmidtchen Artur Schmidtchen |
| author_sort | Michele Tavecchio |
| collection | DOAJ |
| description | Chronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by bioluminescent Staphylococcus aureus. The model addresses previous limitations in studying pressure ulcer infection pathogenesis and evaluating treatment efficacy. By combining this model with in vivo imaging system (IVIS) technology, we achieved real-time, non-invasive monitoring of infection dynamics. This approach demonstrated persistent pressure ulcer wound infection and provided temporal and spatial data on infection status. To validate the model’s utility, we evaluated the antimicrobial efficacy of TCP-25, a synthetic host defense peptide, delivered in a topical gel formulation. Our findings highlight the potential of this model for investigating wound infection mechanisms, bacterial persistence, and therapeutic interventions. This innovative approach represents a significant advancement in pressure ulcer research, offering new opportunities for developing effective treatment strategies and improving patient outcomes. |
| format | Article |
| id | doaj-art-ec73ba1572dd46788522b9cded01966e |
| institution | OA Journals |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-ec73ba1572dd46788522b9cded01966e2025-08-20T01:55:12ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-04-011210.3389/fmed.2025.15617321561732A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infectionMichele Tavecchio0Silvia Fanni1Xuemin Wu2Ganna Petruk3Manoj Puthia4Artur Schmidtchen5Artur Schmidtchen6Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SwedenDermatology, Skåne University Hospital, Lund, SwedenChronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by bioluminescent Staphylococcus aureus. The model addresses previous limitations in studying pressure ulcer infection pathogenesis and evaluating treatment efficacy. By combining this model with in vivo imaging system (IVIS) technology, we achieved real-time, non-invasive monitoring of infection dynamics. This approach demonstrated persistent pressure ulcer wound infection and provided temporal and spatial data on infection status. To validate the model’s utility, we evaluated the antimicrobial efficacy of TCP-25, a synthetic host defense peptide, delivered in a topical gel formulation. Our findings highlight the potential of this model for investigating wound infection mechanisms, bacterial persistence, and therapeutic interventions. This innovative approach represents a significant advancement in pressure ulcer research, offering new opportunities for developing effective treatment strategies and improving patient outcomes.https://www.frontiersin.org/articles/10.3389/fmed.2025.1561732/fullpressure ulcerStaphylococcus aureusTCP-25wound infectionbioimaging |
| spellingShingle | Michele Tavecchio Silvia Fanni Xuemin Wu Ganna Petruk Manoj Puthia Artur Schmidtchen Artur Schmidtchen A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection Frontiers in Medicine pressure ulcer Staphylococcus aureus TCP-25 wound infection bioimaging |
| title | A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection |
| title_full | A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection |
| title_fullStr | A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection |
| title_full_unstemmed | A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection |
| title_short | A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection |
| title_sort | murine pressure ulcer model for evaluating persistence and treatment of staphylococcus aureus infection |
| topic | pressure ulcer Staphylococcus aureus TCP-25 wound infection bioimaging |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1561732/full |
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