Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis
To study the impact of differing specific pathogen-free gut microbiomes (GMs) on a murine model of inflammatory bowel disease, selected GMs were transferred using embryo transfer (ET), cross-fostering (CF), and co-housing (CH). Prior work showed that the GM transfer method and the microbial composit...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2447815 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841527647518064640 |
|---|---|
| author | Kevin L. Gustafson Trevor R. Rodriguez Zachary L. McAdams Lyndon M. Coghill Aaron C. Ericsson Craig L. Franklin |
| author_facet | Kevin L. Gustafson Trevor R. Rodriguez Zachary L. McAdams Lyndon M. Coghill Aaron C. Ericsson Craig L. Franklin |
| author_sort | Kevin L. Gustafson |
| collection | DOAJ |
| description | To study the impact of differing specific pathogen-free gut microbiomes (GMs) on a murine model of inflammatory bowel disease, selected GMs were transferred using embryo transfer (ET), cross-fostering (CF), and co-housing (CH). Prior work showed that the GM transfer method and the microbial composition of donor and recipient GMs can influence microbial colonization and disease phenotypes in dextran sodium sulfate-induced colitis. When a low richness GM was transferred to a recipient with a high richness GM via CH, the donor GM failed to successfully colonize, and a more severe disease phenotype resulted when compared to ET or CF, where colonization was successful. By comparing CH and gastric gavage for fecal material transfer, we isolated the microbial component of this effect and determined that differences in disease severity and survival were associated with microbial factors rather than the transfer method itself. Mice receiving a low richness GM via CH and gastric gavage exhibited greater disease severity and higher expression of pro-inflammatory immune mediators compared to those receiving a high richness GM. This study provides valuable insights into the role of GM composition and colonization in disease modulation. |
| format | Article |
| id | doaj-art-ec731c407641482ebf6aecfb5298cd1f |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-ec731c407641482ebf6aecfb5298cd1f2025-01-15T12:25:19ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2024.2447815Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitisKevin L. Gustafson0Trevor R. Rodriguez1Zachary L. McAdams2Lyndon M. Coghill3Aaron C. Ericsson4Craig L. Franklin5Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, USADepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, USADepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, USADepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, USADepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, USADepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, USATo study the impact of differing specific pathogen-free gut microbiomes (GMs) on a murine model of inflammatory bowel disease, selected GMs were transferred using embryo transfer (ET), cross-fostering (CF), and co-housing (CH). Prior work showed that the GM transfer method and the microbial composition of donor and recipient GMs can influence microbial colonization and disease phenotypes in dextran sodium sulfate-induced colitis. When a low richness GM was transferred to a recipient with a high richness GM via CH, the donor GM failed to successfully colonize, and a more severe disease phenotype resulted when compared to ET or CF, where colonization was successful. By comparing CH and gastric gavage for fecal material transfer, we isolated the microbial component of this effect and determined that differences in disease severity and survival were associated with microbial factors rather than the transfer method itself. Mice receiving a low richness GM via CH and gastric gavage exhibited greater disease severity and higher expression of pro-inflammatory immune mediators compared to those receiving a high richness GM. This study provides valuable insights into the role of GM composition and colonization in disease modulation.https://www.tandfonline.com/doi/10.1080/19490976.2024.2447815Gut microbiota transferdextran sodium sulfate (DSS)DSS-induced colitismicrobiome colonization efficiencyinflammatory bowel disease (IBD)fecal microbiota transfer (FMT) |
| spellingShingle | Kevin L. Gustafson Trevor R. Rodriguez Zachary L. McAdams Lyndon M. Coghill Aaron C. Ericsson Craig L. Franklin Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis Gut Microbes Gut microbiota transfer dextran sodium sulfate (DSS) DSS-induced colitis microbiome colonization efficiency inflammatory bowel disease (IBD) fecal microbiota transfer (FMT) |
| title | Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis |
| title_full | Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis |
| title_fullStr | Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis |
| title_full_unstemmed | Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis |
| title_short | Failure of colonization following gut microbiota transfer exacerbates DSS-induced colitis |
| title_sort | failure of colonization following gut microbiota transfer exacerbates dss induced colitis |
| topic | Gut microbiota transfer dextran sodium sulfate (DSS) DSS-induced colitis microbiome colonization efficiency inflammatory bowel disease (IBD) fecal microbiota transfer (FMT) |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2447815 |
| work_keys_str_mv | AT kevinlgustafson failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis AT trevorrrodriguez failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis AT zacharylmcadams failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis AT lyndonmcoghill failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis AT aaroncericsson failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis AT craiglfranklin failureofcolonizationfollowinggutmicrobiotatransferexacerbatesdssinducedcolitis |