Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients
Inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clini...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/8050432 |
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| author | Mohamed F. El-Badawy Wael M. Tawakol Shaymaa W. El-Far Ibrahim A. Maghrabi Saleh A. Al-Ghamdi Moselhy S. Mansy Mohammed S. Ashour Mohamed M. Shohayeb |
| author_facet | Mohamed F. El-Badawy Wael M. Tawakol Shaymaa W. El-Far Ibrahim A. Maghrabi Saleh A. Al-Ghamdi Moselhy S. Mansy Mohammed S. Ashour Mohamed M. Shohayeb |
| author_sort | Mohamed F. El-Badawy |
| collection | DOAJ |
| description | Inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of Klebsiella pneumoniae. All K. pneumoniae isolates were phenotypically identified using API 20E and then confirmed genotypically through amplification of the specific K. pneumoniae phoE gene. All isolates were genotyped by the enterobacterial repetitive intergenic consensus polymerase chain reaction technique (ERIC-PCR). Antibiotic susceptibility testing was done by a modified Kirby-Bauer method and broth microdilution. All resistant or intermediate-resistant isolates to either gentamicin or amikacin were screened for 7 different genes encoding aminoglycoside-modifying enzymes (AMEs). In addition, all resistant or intermediate-resistant isolates to either ciprofloxacin or levofloxacin were screened for 5 genes encoding the quinolone resistance protein (Qnr), 1 gene encoding quinolone-modifying enzyme, and 3 genes encoding quinolone efflux pumps. Biotyping using API 20E revealed 13 different biotypes. Genotyping demonstrated that all isolates were related to 2 main phylogenetic groups. Susceptibility testing revealed that carbapenems and tigecycline were the most effective agents. Investigation of genes encoding AMEs revealed that acc(6′)-Ib was the most prevalent, followed by acc(3′)-II, aph(3′)-IV, and ant(3′′)-I. Examination of genes encoding Qnr proteins demonstrated that qnrB was the most prevalent, followed by qnrS, qnrD, and qnrC. It was found that 61%, 26%, and 12% of quinolone-resistant K. pneumoniae isolates harbored acc(6′)-Ib-cr, oqxAB, and qebA, respectively. The current study demonstrated a high prevalence of aminoglycoside and quinolone resistance genes among clinical isolates of K. pneumoniae. |
| format | Article |
| id | doaj-art-ec6d6d8d23ca44d9867a8e52158073bf |
| institution | DOAJ |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | International Journal of Microbiology |
| spelling | doaj-art-ec6d6d8d23ca44d9867a8e52158073bf2025-08-20T03:21:26ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/80504328050432Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian PatientsMohamed F. El-Badawy0Wael M. Tawakol1Shaymaa W. El-Far2Ibrahim A. Maghrabi3Saleh A. Al-Ghamdi4Moselhy S. Mansy5Mohammed S. Ashour6Mohamed M. Shohayeb7Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology, Cairo, EgyptGeneral Administration of Pharmaceutical Affairs, Ministry of Health, Alexandria, EgyptDepartment of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, Saudi ArabiaDepartment of Pharmaceutical Microbiology, College of Pharmacy, Taif University, Taif, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDepartment of Pharmaceutical Microbiology, College of Pharmacy, Taif University, Taif, Saudi ArabiaInappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of Klebsiella pneumoniae. All K. pneumoniae isolates were phenotypically identified using API 20E and then confirmed genotypically through amplification of the specific K. pneumoniae phoE gene. All isolates were genotyped by the enterobacterial repetitive intergenic consensus polymerase chain reaction technique (ERIC-PCR). Antibiotic susceptibility testing was done by a modified Kirby-Bauer method and broth microdilution. All resistant or intermediate-resistant isolates to either gentamicin or amikacin were screened for 7 different genes encoding aminoglycoside-modifying enzymes (AMEs). In addition, all resistant or intermediate-resistant isolates to either ciprofloxacin or levofloxacin were screened for 5 genes encoding the quinolone resistance protein (Qnr), 1 gene encoding quinolone-modifying enzyme, and 3 genes encoding quinolone efflux pumps. Biotyping using API 20E revealed 13 different biotypes. Genotyping demonstrated that all isolates were related to 2 main phylogenetic groups. Susceptibility testing revealed that carbapenems and tigecycline were the most effective agents. Investigation of genes encoding AMEs revealed that acc(6′)-Ib was the most prevalent, followed by acc(3′)-II, aph(3′)-IV, and ant(3′′)-I. Examination of genes encoding Qnr proteins demonstrated that qnrB was the most prevalent, followed by qnrS, qnrD, and qnrC. It was found that 61%, 26%, and 12% of quinolone-resistant K. pneumoniae isolates harbored acc(6′)-Ib-cr, oqxAB, and qebA, respectively. The current study demonstrated a high prevalence of aminoglycoside and quinolone resistance genes among clinical isolates of K. pneumoniae.http://dx.doi.org/10.1155/2017/8050432 |
| spellingShingle | Mohamed F. El-Badawy Wael M. Tawakol Shaymaa W. El-Far Ibrahim A. Maghrabi Saleh A. Al-Ghamdi Moselhy S. Mansy Mohammed S. Ashour Mohamed M. Shohayeb Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients International Journal of Microbiology |
| title | Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients |
| title_full | Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients |
| title_fullStr | Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients |
| title_full_unstemmed | Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients |
| title_short | Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients |
| title_sort | molecular identification of aminoglycoside modifying enzymes and plasmid mediated quinolone resistance genes among klebsiella pneumoniae clinical isolates recovered from egyptian patients |
| url | http://dx.doi.org/10.1155/2017/8050432 |
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