<i>Echinococcus multilocularis</i> Calreticulin Inhibits Lectin Pathway of Complement Activation by Directly Binding to Mannose-Binding Lectin

<i>Echinococcus multilocularis</i> Calreticulin Inhibits Lectin Pathway of Complement Activation by Directly Binding to Mannose-Binding Lectin

Alveolar Echinococcosis (AE) is a serious zoonotic disease caused by infection of <i>Echinococcus multilocularis</i> larvae. To survive within the host, <i>E. multilocularis</i> has developed a complex immune evasion mechanism including the inhibition of complement activation...

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Bibliographic Details
Main Authors: Yuxiao Shao, Meng Xia, Yinghui Song, Yan Yan, Xiaofang Dong, Haoran Zong, Bin Zhan, Yanhai Wang, Limei Zhao
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Pathogens
Subjects:
<i>Echinococcus multilocularis</i>
calreticulin
complement lectin activation
mannose-binding lectin
immune evasion
Online Access:https://www.mdpi.com/2076-0817/14/4/354
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Summary:Alveolar Echinococcosis (AE) is a serious zoonotic disease caused by infection of <i>Echinococcus multilocularis</i> larvae. To survive within the host, <i>E. multilocularis</i> has developed a complex immune evasion mechanism including the inhibition of complement activation. This study focused on a calreticulin secreted by <i>E. multilocularis</i> (<i>Em</i>CRT) and its role in binding ability to human MBL and inhibiting MBL-mannose-mediated lectin pathway of complement activation. Results demonstrated the binding of recombinant <i>Em</i>CRT protein to both external and natural MBL in serum and the subsequent inhibition of MBL-mannose-initiated lectin pathway reflected by the reduced formation of complement intermediate products C3b and C4b. Fragment mapping determined that the MBL binding site was located within the S-domain of <i>Em</i>CRT. Combining with its role in inhibiting C1q-initiated classical complement activation in our previous study, the inhibition of MBL-mannose-initiated lectin pathway identified in this study suggests <i>Em</i>CRT plays an important role in the immune evasion of <i>E. multilocularis</i> alveolar larvae against host complement attack as a survival strategy within human tissue. This study supports the approach of using <i>Em</i>CRT as a good candidate for vaccine and drug development against <i>E. multilocularis</i> infection.
ISSN:2076-0817

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