Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i>
Methyl farnesoate (MF), a counterpart of insect juvenile hormones in crustaceans, plays essential roles in molting, metamorphosis, and reproduction. In this paper, we isolated a gene-encoding Krüppel homolog 1 from the mud crab <i>Scylla paramamosain</i> (<i>Sp-Kr-h1</i>) and...
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2025-02-01
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| author | Yongqi Lai Li Lu Shaoming Gong Fang Liu Haihui Ye |
| author_facet | Yongqi Lai Li Lu Shaoming Gong Fang Liu Haihui Ye |
| author_sort | Yongqi Lai |
| collection | DOAJ |
| description | Methyl farnesoate (MF), a counterpart of insect juvenile hormones in crustaceans, plays essential roles in molting, metamorphosis, and reproduction. In this paper, we isolated a gene-encoding Krüppel homolog 1 from the mud crab <i>Scylla paramamosain</i> (<i>Sp-Kr-h1</i>) and investigated its involvement in MF-regulated vitellogenesis. RT-PCR showed that <i>Sp-Kr-h1</i> had a wide expression in various tissues. qRT-PCR showed that its expression level in the ovary peaked at stage III. Both in vitro and in vivo experiments suggested that the expression of <i>Sp-Kr-h1</i> and <i>Vitellogenin</i> (<i>Vg</i>) in the hepatopancreas was significantly induced by MF administration. Further, the RNA interference technique was employed to illustrate the precise role of <i>Sp-Kr-h1</i> in ovarian development. This revealed that the in vitro silencing of <i>Sp-Kr-h1</i> significantly decreased the level of <i>Vg</i> transcripts located in the hepatopancreas. Meanwhile, an in vivo experiment demonstrated that oocyte growth was inhibited after the knockdown of <i>Sp-Kr-h1</i> in female <i>S. paramamosain</i>. In conclusion, this study identified a Kr-h1 gene in <i>S. paramamosain</i> and demonstrated that it is an essential factor for MF-regulated vitellogenesis. Our results provided a new insight into the mechanism underlying MF inducing ovarian development in mud crabs. |
| format | Article |
| id | doaj-art-ec6a609fe69547ec98df814874abcf97 |
| institution | DOAJ |
| issn | 2410-3888 |
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| publishDate | 2025-02-01 |
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| spelling | doaj-art-ec6a609fe69547ec98df814874abcf972025-08-20T02:42:32ZengMDPI AGFishes2410-38882025-02-0110310310.3390/fishes10030103Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i>Yongqi Lai0Li Lu1Shaoming Gong2Fang Liu3Haihui Ye4State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaMethyl farnesoate (MF), a counterpart of insect juvenile hormones in crustaceans, plays essential roles in molting, metamorphosis, and reproduction. In this paper, we isolated a gene-encoding Krüppel homolog 1 from the mud crab <i>Scylla paramamosain</i> (<i>Sp-Kr-h1</i>) and investigated its involvement in MF-regulated vitellogenesis. RT-PCR showed that <i>Sp-Kr-h1</i> had a wide expression in various tissues. qRT-PCR showed that its expression level in the ovary peaked at stage III. Both in vitro and in vivo experiments suggested that the expression of <i>Sp-Kr-h1</i> and <i>Vitellogenin</i> (<i>Vg</i>) in the hepatopancreas was significantly induced by MF administration. Further, the RNA interference technique was employed to illustrate the precise role of <i>Sp-Kr-h1</i> in ovarian development. This revealed that the in vitro silencing of <i>Sp-Kr-h1</i> significantly decreased the level of <i>Vg</i> transcripts located in the hepatopancreas. Meanwhile, an in vivo experiment demonstrated that oocyte growth was inhibited after the knockdown of <i>Sp-Kr-h1</i> in female <i>S. paramamosain</i>. In conclusion, this study identified a Kr-h1 gene in <i>S. paramamosain</i> and demonstrated that it is an essential factor for MF-regulated vitellogenesis. Our results provided a new insight into the mechanism underlying MF inducing ovarian development in mud crabs.https://www.mdpi.com/2410-3888/10/3/103methyl farnesoateKrüppel homolog 1ovarian developmentvitellogenesismud crab |
| spellingShingle | Yongqi Lai Li Lu Shaoming Gong Fang Liu Haihui Ye Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> Fishes methyl farnesoate Krüppel homolog 1 ovarian development vitellogenesis mud crab |
| title | Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> |
| title_full | Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> |
| title_fullStr | Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> |
| title_full_unstemmed | Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> |
| title_short | Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i> |
| title_sort | kruppel homolog 1 is required for the role of methyl farnesoate in vitellogenesis in the mud crab i scylla paramamosain i |
| topic | methyl farnesoate Krüppel homolog 1 ovarian development vitellogenesis mud crab |
| url | https://www.mdpi.com/2410-3888/10/3/103 |
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