Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i>

Krüppel Homolog 1 Is Required for the Role of Methyl Farnesoate in Vitellogenesis in the Mud Crab <i>Scylla paramamosain</i>

Methyl farnesoate (MF), a counterpart of insect juvenile hormones in crustaceans, plays essential roles in molting, metamorphosis, and reproduction. In this paper, we isolated a gene-encoding Krüppel homolog 1 from the mud crab <i>Scylla paramamosain</i> (<i>Sp-Kr-h1</i>) and...

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Bibliographic Details
Main Authors: Yongqi Lai, Li Lu, Shaoming Gong, Fang Liu, Haihui Ye
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Fishes
Subjects:
methyl farnesoate
Krüppel homolog 1
ovarian development
vitellogenesis
mud crab
Online Access:https://www.mdpi.com/2410-3888/10/3/103
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Summary:Methyl farnesoate (MF), a counterpart of insect juvenile hormones in crustaceans, plays essential roles in molting, metamorphosis, and reproduction. In this paper, we isolated a gene-encoding Krüppel homolog 1 from the mud crab <i>Scylla paramamosain</i> (<i>Sp-Kr-h1</i>) and investigated its involvement in MF-regulated vitellogenesis. RT-PCR showed that <i>Sp-Kr-h1</i> had a wide expression in various tissues. qRT-PCR showed that its expression level in the ovary peaked at stage III. Both in vitro and in vivo experiments suggested that the expression of <i>Sp-Kr-h1</i> and <i>Vitellogenin</i> (<i>Vg</i>) in the hepatopancreas was significantly induced by MF administration. Further, the RNA interference technique was employed to illustrate the precise role of <i>Sp-Kr-h1</i> in ovarian development. This revealed that the in vitro silencing of <i>Sp-Kr-h1</i> significantly decreased the level of <i>Vg</i> transcripts located in the hepatopancreas. Meanwhile, an in vivo experiment demonstrated that oocyte growth was inhibited after the knockdown of <i>Sp-Kr-h1</i> in female <i>S. paramamosain</i>. In conclusion, this study identified a Kr-h1 gene in <i>S. paramamosain</i> and demonstrated that it is an essential factor for MF-regulated vitellogenesis. Our results provided a new insight into the mechanism underlying MF inducing ovarian development in mud crabs.
ISSN:2410-3888

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