Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia
Background and Aim: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing...
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Veterinary World
2025-01-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/January-2025/18.pdf |
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| author | Fidi Nur Aini Eka Puji Dameanti Sheila Marty Yanestria Mustofa Helmi Effendi Hani Plumeriastuti Wiwiek Tyasningsih Emmanuel Nnabuike Ugbo Rahayu Sutrisno Muhammad Ali Akramsyah Safri |
| author_facet | Fidi Nur Aini Eka Puji Dameanti Sheila Marty Yanestria Mustofa Helmi Effendi Hani Plumeriastuti Wiwiek Tyasningsih Emmanuel Nnabuike Ugbo Rahayu Sutrisno Muhammad Ali Akramsyah Safri |
| author_sort | Fidi Nur Aini Eka Puji Dameanti |
| collection | DOAJ |
| description | Background and Aim: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing E. coli isolates harboring blaCTX-M, blaTEM, and blaSHV genes from dairy farm wastewater in East Java, Indonesia.
Materials and Methods: Wastewater samples (n = 342) were collected from six major dairy regions in East Java: Pasuruan, Malang, Tulungagung, Blitar, Batu, and Kediri. The samples underwent bacterial isolation, identification, and ESBL phenotypic screening using the double-disk synergy test (DDST). Molecular genotyping of blaCTX-M, blaTEM, and blaSHV was conducted using a polymerase chain reaction.
Results: Of the samples, 69.30% (237/342) tested positive for E. coli, with 32.91% (78/237) identified as ESBL producers through DDST. Molecular genotyping confirmed ESBL genes in 28.20% (22/78) of the isolates. The genotypic distribution was 77.2% (17/22) blaTEM, 4.5% (1/22) blaCTX-M, 9.0% (2/22) blaTEM + blaSHV, and 9.0% (2/22) blaTEM + blaCTX-M. These findings highlight the dominance of blaTEM and the presence of multi-gene combinations in East Java’s dairy farm wastewater.
Conclusion: The identified molecular profiles underscore dairy farm wastewater as a critical reservoir for antibiotic resistance genes, particularly blaTEM. Addressing this issue necessitates stringent antimicrobial use policies, improved wastewater management, and enhanced biosecurity measures. These findings support a One Health approach to mitigate environmental and public health risks associated with AMR. |
| format | Article |
| id | doaj-art-ec65c3bef475462e985caa8546f66ebe |
| institution | Kabale University |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj-art-ec65c3bef475462e985caa8546f66ebe2025-02-07T12:20:48ZengVeterinary WorldVeterinary World0972-89882231-09162025-01-0118116217110.14202/vetworld.2025.162-171Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, IndonesiaFidi Nur Aini Eka Puji Dameanti0https://orcid.org/0000-0002-7928-1073Sheila Marty Yanestria1https://orcid.org/0000-0002-2514-1104Mustofa Helmi Effendi2https://orcid.org/0000-0001-9727-411XHani Plumeriastuti3https://orcid.org/0000-0002-4540-811XWiwiek Tyasningsih4https://orcid.org/0000-0003-0604-8534Emmanuel Nnabuike Ugbo5https://orcid.org/0000-0002-0200-481XRahayu Sutrisno6Muhammad Ali Akramsyah Safri7https://orcid.org/0009-0007-7244-8372Laboratory of Microbiology and Immunology Veterinary, Faculty of Veterinary Medicine, Universitas Brawijaya. Jl. Puncak Dieng, Kalisongo, Malang Regency, 65151. East Java, Indonesia.Department of Veterinary Public Health, Wijaya Kusuma Surabaya University, Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Dukuhpakis, Surabaya, 60225, East Java, Indonesia.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia.Department of Applied Microbiology, Faculty of Science, Ebonyi State University. 480211, Abakaliki, Nigeria.Laboratory of Microbiology and Immunology Veterinary, Faculty of Veterinary Medicine, Universitas Brawijaya. Jl. Puncak Dieng, Kalisongo, Malang Regency, 65151. East Java, Indonesia.Department of Animal Health, Unggas Karya Mandiri, Malang Regency 65151. East Java, Indonesia.Background and Aim: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing E. coli isolates harboring blaCTX-M, blaTEM, and blaSHV genes from dairy farm wastewater in East Java, Indonesia. Materials and Methods: Wastewater samples (n = 342) were collected from six major dairy regions in East Java: Pasuruan, Malang, Tulungagung, Blitar, Batu, and Kediri. The samples underwent bacterial isolation, identification, and ESBL phenotypic screening using the double-disk synergy test (DDST). Molecular genotyping of blaCTX-M, blaTEM, and blaSHV was conducted using a polymerase chain reaction. Results: Of the samples, 69.30% (237/342) tested positive for E. coli, with 32.91% (78/237) identified as ESBL producers through DDST. Molecular genotyping confirmed ESBL genes in 28.20% (22/78) of the isolates. The genotypic distribution was 77.2% (17/22) blaTEM, 4.5% (1/22) blaCTX-M, 9.0% (2/22) blaTEM + blaSHV, and 9.0% (2/22) blaTEM + blaCTX-M. These findings highlight the dominance of blaTEM and the presence of multi-gene combinations in East Java’s dairy farm wastewater. Conclusion: The identified molecular profiles underscore dairy farm wastewater as a critical reservoir for antibiotic resistance genes, particularly blaTEM. Addressing this issue necessitates stringent antimicrobial use policies, improved wastewater management, and enhanced biosecurity measures. These findings support a One Health approach to mitigate environmental and public health risks associated with AMR.https://www.veterinaryworld.org/Vol.18/January-2025/18.pdfantibiotic resistanceblactx-mblashvblatemdairy farm wastewateresbl-producing escherichia coliindonesiapublic health |
| spellingShingle | Fidi Nur Aini Eka Puji Dameanti Sheila Marty Yanestria Mustofa Helmi Effendi Hani Plumeriastuti Wiwiek Tyasningsih Emmanuel Nnabuike Ugbo Rahayu Sutrisno Muhammad Ali Akramsyah Safri Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia Veterinary World antibiotic resistance blactx-m blashv blatem dairy farm wastewater esbl-producing escherichia coli indonesia public health |
| title | Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia |
| title_full | Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia |
| title_fullStr | Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia |
| title_full_unstemmed | Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia |
| title_short | Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia |
| title_sort | genotyping of extended spectrum beta lactamase producing escherichia coli isolated from wastewater of dairy farms in east java indonesia |
| topic | antibiotic resistance blactx-m blashv blatem dairy farm wastewater esbl-producing escherichia coli indonesia public health |
| url | https://www.veterinaryworld.org/Vol.18/January-2025/18.pdf |
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