RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy
Graves’ ophthalmopathy (GO) is a chronic autoimmune inflammatory disorder involving orbital tissues. A receptor for advanced glycation end products (RAGE) and its ligand high mobility group box 1 (HMGB1) protein trigger inflammation and cell proliferation and are involved in the pathogenesis of vari...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/8891324 |
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| author | Dominika Łacheta Krzysztof B. Poślednik Katarzyna Czerwaty Nils Ludwig Marta Molińska-Glura Ireneusz Kantor Anna Jabłońska-Pawlak Piotr Miśkiewicz Alicja Głuszko Zygmunt Stopa Jacek Brzost Mirosław J. Szczepański |
| author_facet | Dominika Łacheta Krzysztof B. Poślednik Katarzyna Czerwaty Nils Ludwig Marta Molińska-Glura Ireneusz Kantor Anna Jabłońska-Pawlak Piotr Miśkiewicz Alicja Głuszko Zygmunt Stopa Jacek Brzost Mirosław J. Szczepański |
| author_sort | Dominika Łacheta |
| collection | DOAJ |
| description | Graves’ ophthalmopathy (GO) is a chronic autoimmune inflammatory disorder involving orbital tissues. A receptor for advanced glycation end products (RAGE) and its ligand high mobility group box 1 (HMGB1) protein trigger inflammation and cell proliferation and are involved in the pathogenesis of various chronic inflammatory diseases. This study was aimed to evaluate RAGE and HMGB1 expression in GO to determine its potential clinical significance. To the best of our knowledge, this is the first study showing RAGE and HMGB1 expression in orbital tissue using immunohistochemistry. Sections of orbital adipose tissue obtained from patients diagnosed with GO (23 patients; 36 orbits) and normal controls (NC) (15 patients; 15 orbits) were analyzed by immunohistochemistry for RAGE and HMGB1 expression. Expression profiles were then correlated with clinical data of the study group. RAGE and HMGB1 expression were elevated in GO patients in comparison with NC (p=0.001 and p=0.02, respectively). We observed a correlation between RAGE expression and occurrence of dysthyroid optic neuropathy (DON) (p=0.05) and levels of TSH Receptor Antibodies (TRAb) (p=0.01). Overexpression of RAGE and HMGB1 might be associated with GO pathogenesis. In addition, RAGE and HMGB1 proteins may be considered as promising therapeutic targets, but this requires further research. |
| format | Article |
| id | doaj-art-ec5de3fc9dfc4e09a4fa3fd31f3ef390 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ec5de3fc9dfc4e09a4fa3fd31f3ef3902025-08-20T03:21:26ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/88913248891324RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ OphthalmopathyDominika Łacheta0Krzysztof B. Poślednik1Katarzyna Czerwaty2Nils Ludwig3Marta Molińska-Glura4Ireneusz Kantor5Anna Jabłońska-Pawlak6Piotr Miśkiewicz7Alicja Głuszko8Zygmunt Stopa9Jacek Brzost10Mirosław J. Szczepański11Department of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, PolandDepartment of Otolaryngology, Medical Centre for Postgraduate Education, 03-242 Warsaw, PolandDepartment of Otolaryngology, Medical Centre for Postgraduate Education, 03-242 Warsaw, PolandDepartment of Oral and Maxillofacial Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Forest Technology of Poznan University of Life Sciences, 60-624 Poznan, PolandDepartment of Otolaryngology, Medical Centre for Postgraduate Education, 03-242 Warsaw, PolandDepartment of Ophthalmology, Medical University of Warsaw, 02-097 Warsaw, PolandDepartment of Internal Medicine and Endocrinology, Medical University of Warsaw, 02-097 Warsaw, PolandDepartment of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, PolandDepartment of Maxillofacial Surgery, Medical University of Warsaw, 02-097 Warsaw, PolandDepartment of Otolaryngology, The Children’s Memorial Health Institute, 04-730 Warsaw, PolandDepartment of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, PolandGraves’ ophthalmopathy (GO) is a chronic autoimmune inflammatory disorder involving orbital tissues. A receptor for advanced glycation end products (RAGE) and its ligand high mobility group box 1 (HMGB1) protein trigger inflammation and cell proliferation and are involved in the pathogenesis of various chronic inflammatory diseases. This study was aimed to evaluate RAGE and HMGB1 expression in GO to determine its potential clinical significance. To the best of our knowledge, this is the first study showing RAGE and HMGB1 expression in orbital tissue using immunohistochemistry. Sections of orbital adipose tissue obtained from patients diagnosed with GO (23 patients; 36 orbits) and normal controls (NC) (15 patients; 15 orbits) were analyzed by immunohistochemistry for RAGE and HMGB1 expression. Expression profiles were then correlated with clinical data of the study group. RAGE and HMGB1 expression were elevated in GO patients in comparison with NC (p=0.001 and p=0.02, respectively). We observed a correlation between RAGE expression and occurrence of dysthyroid optic neuropathy (DON) (p=0.05) and levels of TSH Receptor Antibodies (TRAb) (p=0.01). Overexpression of RAGE and HMGB1 might be associated with GO pathogenesis. In addition, RAGE and HMGB1 proteins may be considered as promising therapeutic targets, but this requires further research.http://dx.doi.org/10.1155/2021/8891324 |
| spellingShingle | Dominika Łacheta Krzysztof B. Poślednik Katarzyna Czerwaty Nils Ludwig Marta Molińska-Glura Ireneusz Kantor Anna Jabłońska-Pawlak Piotr Miśkiewicz Alicja Głuszko Zygmunt Stopa Jacek Brzost Mirosław J. Szczepański RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy Mediators of Inflammation |
| title | RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy |
| title_full | RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy |
| title_fullStr | RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy |
| title_full_unstemmed | RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy |
| title_short | RAGE and HMGB1 Expression in Orbital Tissue Microenvironment in Graves’ Ophthalmopathy |
| title_sort | rage and hmgb1 expression in orbital tissue microenvironment in graves ophthalmopathy |
| url | http://dx.doi.org/10.1155/2021/8891324 |
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