Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer
Abstract ALDH1 isotype is an established cancer stem cell marker. In this study, immunohistochemical (IHC) expression of ALDH1/2 and ALDH1A3 protein was evaluated in a retrospective cohort of 99 pathologically confirmed MIBC patients and 11 distant normal bladder urothelium. IHC expression was asses...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-82460-1 |
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| author | Sumit Dey Smita Pattanaik Nandita Kakkar Monidipa Konar Ipsita Panda Arup Kumar Mandal Ravimohan Suryanarayan Mavuduru |
| author_facet | Sumit Dey Smita Pattanaik Nandita Kakkar Monidipa Konar Ipsita Panda Arup Kumar Mandal Ravimohan Suryanarayan Mavuduru |
| author_sort | Sumit Dey |
| collection | DOAJ |
| description | Abstract ALDH1 isotype is an established cancer stem cell marker. In this study, immunohistochemical (IHC) expression of ALDH1/2 and ALDH1A3 protein was evaluated in a retrospective cohort of 99 pathologically confirmed MIBC patients and 11 distant normal bladder urothelium. IHC expression was assessed quantitatively using histopathology score (H score). H score of ≥ 4 was graded as a high expression. Gene expression of ALDH1A1, 1A2 and 1A3 along with immunoblot procedure was also performed in a prospectively collected 30 MIBC patients to further validate the IHC findings. Expression of both ALDH1/2 and ALDH1A3 were found to be significantly higher in MIBC tumors than in the normal bladder urothelium. Tumors with higher T stage showed a significant association with ALDH1A3 expression (p = 0.002). Patients with a high H score of ALDH1A3 protein survived for significantly less (p = 0.008) duration of time (20 months) than patients with low score (34 months). Gene expression profile of ALDH1 isotypes revealed that high mRNA expression of ALDH1A3 was significantly associated with less overall survival which was further validated separately using public dataset. Our results indicate that ALDH1 isotypes have the potential to be used as an effective diagnostic and prognostic marker in patients with MIBC. |
| format | Article |
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| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-ec53487ac29147ef82d8fcb5f6804ed22025-08-20T03:03:25ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-024-82460-1Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancerSumit Dey0Smita Pattanaik1Nandita Kakkar2Monidipa Konar3Ipsita Panda4Arup Kumar Mandal5Ravimohan Suryanarayan Mavuduru6Department of Urology, Post Graduate Institute of Medical Education and Research (PGIMER)Department of Pharmacology, Post Graduate Institute of Medical Education and Research (PGIMER)Department of Histopathology, Post Graduate Institute of Medical Education and Research (PGIMER)Department of Biochemistry, Post Graduate Institute of Medical Education and Research (PGIMER)Department of Histopathology, Post Graduate Institute of Medical Education and Research (PGIMER)Department of Urology, All India Institute of Medical Sciences (AIIMS)Department of Urology, Post Graduate Institute of Medical Education and Research (PGIMER)Abstract ALDH1 isotype is an established cancer stem cell marker. In this study, immunohistochemical (IHC) expression of ALDH1/2 and ALDH1A3 protein was evaluated in a retrospective cohort of 99 pathologically confirmed MIBC patients and 11 distant normal bladder urothelium. IHC expression was assessed quantitatively using histopathology score (H score). H score of ≥ 4 was graded as a high expression. Gene expression of ALDH1A1, 1A2 and 1A3 along with immunoblot procedure was also performed in a prospectively collected 30 MIBC patients to further validate the IHC findings. Expression of both ALDH1/2 and ALDH1A3 were found to be significantly higher in MIBC tumors than in the normal bladder urothelium. Tumors with higher T stage showed a significant association with ALDH1A3 expression (p = 0.002). Patients with a high H score of ALDH1A3 protein survived for significantly less (p = 0.008) duration of time (20 months) than patients with low score (34 months). Gene expression profile of ALDH1 isotypes revealed that high mRNA expression of ALDH1A3 was significantly associated with less overall survival which was further validated separately using public dataset. Our results indicate that ALDH1 isotypes have the potential to be used as an effective diagnostic and prognostic marker in patients with MIBC.https://doi.org/10.1038/s41598-024-82460-1 |
| spellingShingle | Sumit Dey Smita Pattanaik Nandita Kakkar Monidipa Konar Ipsita Panda Arup Kumar Mandal Ravimohan Suryanarayan Mavuduru Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer Scientific Reports |
| title | Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| title_full | Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| title_fullStr | Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| title_full_unstemmed | Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| title_short | Expression of ALDH1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| title_sort | expression of aldh1 isotypes and its potential as a prognostic and diagnostic marker in patients with muscle invasive bladder cancer |
| url | https://doi.org/10.1038/s41598-024-82460-1 |
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