Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)

Objectives: Antimicrobial resistance (AMR) is a major public health concern worldwide. International travel is a risk factor for acquiring antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). Therefore, understanding the transmission of ARB and ARGs is instrumental in tackling...

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Main Authors: Mélanie Pimenta, Maria Alexa, Degrâce Batantou Mabandza, Sylvain Dulaurent, Bich-Tram Huynh, Margaux Gaschet, Lulla Opatowski, Sébastien Breurec, Marie-Cécile Ploy, Christophe Dagot
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Journal of Global Antimicrobial Resistance
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213716525000700
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author Mélanie Pimenta
Maria Alexa
Degrâce Batantou Mabandza
Sylvain Dulaurent
Bich-Tram Huynh
Margaux Gaschet
Lulla Opatowski
Sébastien Breurec
Marie-Cécile Ploy
Christophe Dagot
author_facet Mélanie Pimenta
Maria Alexa
Degrâce Batantou Mabandza
Sylvain Dulaurent
Bich-Tram Huynh
Margaux Gaschet
Lulla Opatowski
Sébastien Breurec
Marie-Cécile Ploy
Christophe Dagot
author_sort Mélanie Pimenta
collection DOAJ
description Objectives: Antimicrobial resistance (AMR) is a major public health concern worldwide. International travel is a risk factor for acquiring antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). Therefore, understanding the transmission of ARB and ARGs is instrumental in tackling AMR. This longitudinal study aimed to assess the benefit of wastewater monitoring in Guadeloupe to evaluate the role of tourism in the spread of AMR. Methods: A wastewater-based surveillance (WBS) study was conducted to monitor AMR in Guadeloupe in 2022 during dry and wet seasons. We characterized the resistome, microbiome and exposome of water samples collected in wastewater treatment facilities of two cities with different levels of tourism activities, in the content of aircraft toilets, and the pumping station receiving effluents from hotels. Results: The results show that the WBS approach facilitates the differentiation of various untreated effluents concerning exposome, microbiome, and resistome, offering insights into AMR dissemination. Additionally, the findings reveal that microbiome and exposome are comparable across sites and seasons, while resistome characterisation at specific locations may be pertinent for health surveillance. The microbiome of aircraft was predominantly composed of anaerobic bacteria from human intestinal microbiota, whereas the other locations exhibited a blend of human and environmental bacteria. Notably, individuals arriving by air have not introduced clinically significant resistance genes. Exposome compounds have been shown to influence the resistome's variance. Conclusions: Clear differences were seen between the aircraft and the local sampling sites, indicating that the contribution of tourism to the observed resistance in Guadeloupe is not significant.
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spelling doaj-art-ec3d901ef2b349a58076d7c76781bed72025-08-20T03:30:32ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-06-0143273410.1016/j.jgar.2025.03.010Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)Mélanie Pimenta0Maria Alexa1Degrâce Batantou Mabandza2Sylvain Dulaurent3Bich-Tram Huynh4Margaux Gaschet5Lulla Opatowski6Sébastien Breurec7Marie-Cécile Ploy8Christophe Dagot9INSERM, CHU Limoges, RESINFIT, U1092, University of Limoges, Limoges, FranceInstitut Pasteur, Université Paris-Cité, Epidemiology and Modelling of Antibiotic Evasion (EMAE), France; Anti-infective Evasion and Pharmacoepidemiology Team, CESP, Université Paris-Saclay, UVSQ, INSERM, Montigny-le-Bretonneux, FranceTransmission, Reservoir and Diversity of Pathogens Unit, Pasteur Institute of Guadeloupe, Pointe-à-Pitre, FranceDepartment of Pharmacology, Toxicology and Pharmacovigilance, Limoges University Hospital, Limoges, FranceInstitut Pasteur, Université Paris-Cité, Epidemiology and Modelling of Antibiotic Evasion (EMAE), France; Anti-infective Evasion and Pharmacoepidemiology Team, CESP, Université Paris-Saclay, UVSQ, INSERM, Montigny-le-Bretonneux, FranceINSERM, CHU Limoges, RESINFIT, U1092, University of Limoges, Limoges, FranceInstitut Pasteur, Université Paris-Cité, Epidemiology and Modelling of Antibiotic Evasion (EMAE), France; Anti-infective Evasion and Pharmacoepidemiology Team, CESP, Université Paris-Saclay, UVSQ, INSERM, Montigny-le-Bretonneux, FranceTransmission, Reservoir and Diversity of Pathogens Unit, Pasteur Institute of Guadeloupe, Pointe-à-Pitre, France; Faculty of Medicine Hyacinthe Bastaraud, University of Antilles, Pointe-à-Pitre, France; Centre for Clinical Investigation 1424, INSERM, Pointe-à-Pitre/Les Abymes, France; Laboratory of clinical microbiology, University hospital Center of Guadeloupe, Pointe-à-Pitre/Les Abymes, FranceINSERM, CHU Limoges, RESINFIT, U1092, University of Limoges, Limoges, FranceINSERM, CHU Limoges, RESINFIT, U1092, University of Limoges, Limoges, France; Corresponding author.Objectives: Antimicrobial resistance (AMR) is a major public health concern worldwide. International travel is a risk factor for acquiring antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). Therefore, understanding the transmission of ARB and ARGs is instrumental in tackling AMR. This longitudinal study aimed to assess the benefit of wastewater monitoring in Guadeloupe to evaluate the role of tourism in the spread of AMR. Methods: A wastewater-based surveillance (WBS) study was conducted to monitor AMR in Guadeloupe in 2022 during dry and wet seasons. We characterized the resistome, microbiome and exposome of water samples collected in wastewater treatment facilities of two cities with different levels of tourism activities, in the content of aircraft toilets, and the pumping station receiving effluents from hotels. Results: The results show that the WBS approach facilitates the differentiation of various untreated effluents concerning exposome, microbiome, and resistome, offering insights into AMR dissemination. Additionally, the findings reveal that microbiome and exposome are comparable across sites and seasons, while resistome characterisation at specific locations may be pertinent for health surveillance. The microbiome of aircraft was predominantly composed of anaerobic bacteria from human intestinal microbiota, whereas the other locations exhibited a blend of human and environmental bacteria. Notably, individuals arriving by air have not introduced clinically significant resistance genes. Exposome compounds have been shown to influence the resistome's variance. Conclusions: Clear differences were seen between the aircraft and the local sampling sites, indicating that the contribution of tourism to the observed resistance in Guadeloupe is not significant.http://www.sciencedirect.com/science/article/pii/S2213716525000700Antimicrobial resistanceWastewaterTourismGuadeloupe
spellingShingle Mélanie Pimenta
Maria Alexa
Degrâce Batantou Mabandza
Sylvain Dulaurent
Bich-Tram Huynh
Margaux Gaschet
Lulla Opatowski
Sébastien Breurec
Marie-Cécile Ploy
Christophe Dagot
Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
Journal of Global Antimicrobial Resistance
Antimicrobial resistance
Wastewater
Tourism
Guadeloupe
title Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
title_full Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
title_fullStr Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
title_full_unstemmed Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
title_short Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)
title_sort wastewater based amr surveillance associated with tourism on a caribbean island guadeloupe
topic Antimicrobial resistance
Wastewater
Tourism
Guadeloupe
url http://www.sciencedirect.com/science/article/pii/S2213716525000700
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