OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance
Abstract Aquaporin (AQP)-dependent water transport across membranes is indispensable in plants. Recent evidence shows that several AQPs, including plasma membrane intrinsic proteins (PIPs), facilitate the electrogenic transport of ions as well as water transport and are referred to as ion-conducting...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-96259-1 |
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| author | Sen Thi Huong Tran Maki Katsuhara Yunosuke Mito Aya Onishi Ayaka Higa Shuntaro Ono Newton Chandra Paul Rie Horie Yoshihiko Harada Tomoaki Horie |
| author_facet | Sen Thi Huong Tran Maki Katsuhara Yunosuke Mito Aya Onishi Ayaka Higa Shuntaro Ono Newton Chandra Paul Rie Horie Yoshihiko Harada Tomoaki Horie |
| author_sort | Sen Thi Huong Tran |
| collection | DOAJ |
| description | Abstract Aquaporin (AQP)-dependent water transport across membranes is indispensable in plants. Recent evidence shows that several AQPs, including plasma membrane intrinsic proteins (PIPs), facilitate the electrogenic transport of ions as well as water transport and are referred to as ion-conducting aquaporins (icAQPs). The present study attempted to identify icAQPs that exhibit cation transport activity among PIPs from rice. Electrophysiological experiments on 11 OsPIPs using Xenopus laevis oocytes revealed that OsPIP2;4 mediated the electrogenic transport of alkali monovalent cations with the selectivity sequence of Na+ ≈ K+ > Rb+ > Cs+ > Li+, suggesting non-selective cation conductance for Na+ and K+. Transcripts of OsPIP2;4 were abundant in the elongation and mature zones of roots with similar expression levels between the root stelar and remaining outer parts in the cultivar Nipponbare. Immunostaining using sections of the crown roots of Nipponbare plants revealed the expression of OsPIP2;4 in the exodermis and sclerenchyma of the surface region and in the endodermis and pericycle of the stelar region. The present results provide novel insights into OsPIP2;4-dependent non-selective Na+ and K+ transport and its physiological roles in rice. |
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| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-ec3d3a53a2e448f8b7c2924e57e627502025-08-20T02:24:29ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-96259-1OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductanceSen Thi Huong Tran0Maki Katsuhara1Yunosuke Mito2Aya Onishi3Ayaka Higa4Shuntaro Ono5Newton Chandra Paul6Rie Horie7Yoshihiko Harada8Tomoaki Horie9Institute of Plant Science and Resources, Okayama UniversityInstitute of Plant Science and Resources, Okayama UniversityDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu UniversityInstitute of Plant Science and Resources, Okayama UniversityDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu UniversityInstitute of Plant Science and Resources, Okayama UniversityInstitute of Plant Science and Resources, Okayama UniversityDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu UniversityDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu UniversityDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu UniversityAbstract Aquaporin (AQP)-dependent water transport across membranes is indispensable in plants. Recent evidence shows that several AQPs, including plasma membrane intrinsic proteins (PIPs), facilitate the electrogenic transport of ions as well as water transport and are referred to as ion-conducting aquaporins (icAQPs). The present study attempted to identify icAQPs that exhibit cation transport activity among PIPs from rice. Electrophysiological experiments on 11 OsPIPs using Xenopus laevis oocytes revealed that OsPIP2;4 mediated the electrogenic transport of alkali monovalent cations with the selectivity sequence of Na+ ≈ K+ > Rb+ > Cs+ > Li+, suggesting non-selective cation conductance for Na+ and K+. Transcripts of OsPIP2;4 were abundant in the elongation and mature zones of roots with similar expression levels between the root stelar and remaining outer parts in the cultivar Nipponbare. Immunostaining using sections of the crown roots of Nipponbare plants revealed the expression of OsPIP2;4 in the exodermis and sclerenchyma of the surface region and in the endodermis and pericycle of the stelar region. The present results provide novel insights into OsPIP2;4-dependent non-selective Na+ and K+ transport and its physiological roles in rice.https://doi.org/10.1038/s41598-025-96259-1Ion-conducting AquaporinsNon-selective cation channelRiceRoots |
| spellingShingle | Sen Thi Huong Tran Maki Katsuhara Yunosuke Mito Aya Onishi Ayaka Higa Shuntaro Ono Newton Chandra Paul Rie Horie Yoshihiko Harada Tomoaki Horie OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance Scientific Reports Ion-conducting Aquaporins Non-selective cation channel Rice Roots |
| title | OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance |
| title_full | OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance |
| title_fullStr | OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance |
| title_full_unstemmed | OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance |
| title_short | OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance |
| title_sort | ospip2 4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective na and k conductance |
| topic | Ion-conducting Aquaporins Non-selective cation channel Rice Roots |
| url | https://doi.org/10.1038/s41598-025-96259-1 |
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