Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region
Aim. The work is aimed at exploring the molecular and genetic properties of avian paramyxovirus 4 (APMV‐4) isolated from the mallard duck. Material and Methods. In 2018, as a part of an influenza A virus surveillance programme, biological samples were collected from wild birds on Lake Adzhi (Papas)...
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Kamerton
2021-10-01
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| Series: | Юг России: экология, развитие |
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| Online Access: | https://ecodag.elpub.ru/ugro/article/view/2281 |
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| author | A. A. Derko N. A. Dubovitskiy T. A. Murashkina I. A. Sobolev M. V. Solomatina A. Yu. Alekseev M. G. Magomedov J. Mine Yu. Uchida T. Saito M. M. Kallaeva K. A. Sharshov |
| author_facet | A. A. Derko N. A. Dubovitskiy T. A. Murashkina I. A. Sobolev M. V. Solomatina A. Yu. Alekseev M. G. Magomedov J. Mine Yu. Uchida T. Saito M. M. Kallaeva K. A. Sharshov |
| author_sort | A. A. Derko |
| collection | DOAJ |
| description | Aim. The work is aimed at exploring the molecular and genetic properties of avian paramyxovirus 4 (APMV‐4) isolated from the mallard duck. Material and Methods. In 2018, as a part of an influenza A virus surveillance programme, biological samples were collected from wild birds on Lake Adzhi (Papas) in the Republic of Dagestan. The isolation of virus strains from biological samples was performed using 10‐day‐old special pathogen‐free chicken eggs. The primary detection of hemagglutinating agents in the fluid of the allantoic cavity was carried out via hemagglutination assay. The presence of RNA of the influenza A virus was determined using Real Time PCR. Then, for samples negative in PCR for the influenza A virus, the complete genome DNA sequences were established using NGS sequencing. It was determined that the APMV‐4 strain obtained belonged to intraspecific genetic lines was determined using phylogenetic analysis.Results. APMV‐4 strain was isolated for the first time in the Western Caspian region. Phylogenetic analysis of the complete genome and gene F showed that the APMV‐4/mallard/Dagestan/92d/2018 strain belongs to the Eurasian clade of subgenotypes. Analysis of amino acid substitutions showed that the cleavage site of the fusion protein is monobasic which is characteristic of lentogenic strains.Conclusion. During the surveillance program for influenza A viruses in autumn 2018, the APMV‐4 strain was isolated from the mallard duck. Analysis revealed that the APMV‐4 strain was closely phylogenetically related to strains isolated from two wild and one domestic duck from China. This suggests an interconnection between the bird populations in China and the Western Caspian region within the West Asian‐East African migration route. |
| format | Article |
| id | doaj-art-ec1e9b98626c4f40a03eab145d79cc9e |
| institution | DOAJ |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| publishDate | 2021-10-01 |
| publisher | Kamerton |
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| series | Юг России: экология, развитие |
| spelling | doaj-art-ec1e9b98626c4f40a03eab145d79cc9e2025-08-20T03:18:42ZrusKamertonЮг России: экология, развитие1992-10982413-09582021-10-0116310.18470/1992-1098-2021-3-81-871184Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian regionA. A. Derko0N. A. Dubovitskiy1T. A. Murashkina2I. A. Sobolev3M. V. Solomatina4A. Yu. Alekseev5M. G. Magomedov6J. Mine7Yu. Uchida8T. Saito9M. M. Kallaeva10K. A. Sharshov11Federal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineDagestan State Medical UniversityNational Institute of Animal HealthNational Institute of Animal HealthNational Institute of Animal HealthDagestan State Medical UniversityFederal Research Centre of Fundamental and Translational MedicineAim. The work is aimed at exploring the molecular and genetic properties of avian paramyxovirus 4 (APMV‐4) isolated from the mallard duck. Material and Methods. In 2018, as a part of an influenza A virus surveillance programme, biological samples were collected from wild birds on Lake Adzhi (Papas) in the Republic of Dagestan. The isolation of virus strains from biological samples was performed using 10‐day‐old special pathogen‐free chicken eggs. The primary detection of hemagglutinating agents in the fluid of the allantoic cavity was carried out via hemagglutination assay. The presence of RNA of the influenza A virus was determined using Real Time PCR. Then, for samples negative in PCR for the influenza A virus, the complete genome DNA sequences were established using NGS sequencing. It was determined that the APMV‐4 strain obtained belonged to intraspecific genetic lines was determined using phylogenetic analysis.Results. APMV‐4 strain was isolated for the first time in the Western Caspian region. Phylogenetic analysis of the complete genome and gene F showed that the APMV‐4/mallard/Dagestan/92d/2018 strain belongs to the Eurasian clade of subgenotypes. Analysis of amino acid substitutions showed that the cleavage site of the fusion protein is monobasic which is characteristic of lentogenic strains.Conclusion. During the surveillance program for influenza A viruses in autumn 2018, the APMV‐4 strain was isolated from the mallard duck. Analysis revealed that the APMV‐4 strain was closely phylogenetically related to strains isolated from two wild and one domestic duck from China. This suggests an interconnection between the bird populations in China and the Western Caspian region within the West Asian‐East African migration route.https://ecodag.elpub.ru/ugro/article/view/2281apmv‐4aavv‐4complete genome sequencemallardwild waterfowlwestern caspian region |
| spellingShingle | A. A. Derko N. A. Dubovitskiy T. A. Murashkina I. A. Sobolev M. V. Solomatina A. Yu. Alekseev M. G. Magomedov J. Mine Yu. Uchida T. Saito M. M. Kallaeva K. A. Sharshov Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region Юг России: экология, развитие apmv‐4 aavv‐4 complete genome sequence mallard wild waterfowl western caspian region |
| title | Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region |
| title_full | Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region |
| title_fullStr | Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region |
| title_full_unstemmed | Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region |
| title_short | Avian paramyxovirus 4 isolated from the mallard (<i>Anas platyrhynchos</i>, Linnaeus, 1758): the first case detected in the Western Caspian region |
| title_sort | avian paramyxovirus 4 isolated from the mallard i anas platyrhynchos i linnaeus 1758 the first case detected in the western caspian region |
| topic | apmv‐4 aavv‐4 complete genome sequence mallard wild waterfowl western caspian region |
| url | https://ecodag.elpub.ru/ugro/article/view/2281 |
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