Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates

IntroductionThe global increase in antimicrobial-resistant (AMR) Escherichia coli in the poultry supply chain poses significant food safety and public health risks. This study aims to assess the AMR profiles and plasmid content of E. coli isolated from imported frozen broiler carcasses available in...

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Main Authors: Ihab Habib, Mohamed-Yousif Ibrahim Mohamed, Glindya Bhagya Lakshmi, Akela Ghazawi, Mushtaq Khan, Hazim O. Khalifa
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590906/full
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author Ihab Habib
Ihab Habib
Mohamed-Yousif Ibrahim Mohamed
Mohamed-Yousif Ibrahim Mohamed
Glindya Bhagya Lakshmi
Glindya Bhagya Lakshmi
Akela Ghazawi
Akela Ghazawi
Mushtaq Khan
Mushtaq Khan
Hazim O. Khalifa
author_facet Ihab Habib
Ihab Habib
Mohamed-Yousif Ibrahim Mohamed
Mohamed-Yousif Ibrahim Mohamed
Glindya Bhagya Lakshmi
Glindya Bhagya Lakshmi
Akela Ghazawi
Akela Ghazawi
Mushtaq Khan
Mushtaq Khan
Hazim O. Khalifa
author_sort Ihab Habib
collection DOAJ
description IntroductionThe global increase in antimicrobial-resistant (AMR) Escherichia coli in the poultry supply chain poses significant food safety and public health risks. This study aims to assess the AMR profiles and plasmid content of E. coli isolated from imported frozen broiler carcasses available in the United Arab Emirates (UAE) market.MethodsA total of 253 frozen whole broiler carcasses imported from Brazil, France, Oman, and Ukraine were screened for the presence of E. coli. Antimicrobial susceptibility testing was conducted on 90 isolates. Whole-genome sequencing (WGS) was performed on 33 representative isolates to analyze sequence types (STs), resistance genes, and plasmid content using the MOB-suite pipeline.ResultsE. coli was detected in 248 out of 253 samples. Resistance to ampicillin (52.2%) and tetracycline (35.6%) was most common, with 68.9% of isolates exhibiting multidrug resistance (MDR). WGS revealed 22 STs, with ST1564 being the most prevalent (12.1%). Clinically relevant ST10 and ST58 were also identified. Extended-spectrum β-lactamase (ESBL) genes blaCTX–M–55 and blaCTX–M–8 predominated, often co-occurring with fluoroquinolone resistance genes qnrS1 and qnrB19. A total of 197 plasmids were identified; 63.6% were classified as conjugative. The most frequent relaxase types were MOBP (37 plasmids) and MOBF (24 plasmids), with IncI-gamma/K1 and IncF plasmids commonly linked to ESBL genes.DiscussionThis study provides one of the first genomic characterizations of plasmid-mediated AMR in poultry-associated E. coli in the Middle East. The high prevalence of MDR and mobile resistance elements underscores the role of international poultry trade in AMR dissemination. These findings highlight the need for strengthened AMR surveillance and improved regulatory control over antibiotic use in poultry production to mitigate public health risks.
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spelling doaj-art-ec1cea411d4c4308bc11baca3317c17e2025-08-20T02:06:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15909061590906Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab EmiratesIhab Habib0Ihab Habib1Mohamed-Yousif Ibrahim Mohamed2Mohamed-Yousif Ibrahim Mohamed3Glindya Bhagya Lakshmi4Glindya Bhagya Lakshmi5Akela Ghazawi6Akela Ghazawi7Mushtaq Khan8Mushtaq Khan9Hazim O. Khalifa10Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesASPIRE Research Institute for Food Security in the Drylands (ARIFSID), United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesASPIRE Research Institute for Food Security in the Drylands (ARIFSID), United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesASPIRE Research Institute for Food Security in the Drylands (ARIFSID), United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesZayed Center for Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesZayed Center for Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesIntroductionThe global increase in antimicrobial-resistant (AMR) Escherichia coli in the poultry supply chain poses significant food safety and public health risks. This study aims to assess the AMR profiles and plasmid content of E. coli isolated from imported frozen broiler carcasses available in the United Arab Emirates (UAE) market.MethodsA total of 253 frozen whole broiler carcasses imported from Brazil, France, Oman, and Ukraine were screened for the presence of E. coli. Antimicrobial susceptibility testing was conducted on 90 isolates. Whole-genome sequencing (WGS) was performed on 33 representative isolates to analyze sequence types (STs), resistance genes, and plasmid content using the MOB-suite pipeline.ResultsE. coli was detected in 248 out of 253 samples. Resistance to ampicillin (52.2%) and tetracycline (35.6%) was most common, with 68.9% of isolates exhibiting multidrug resistance (MDR). WGS revealed 22 STs, with ST1564 being the most prevalent (12.1%). Clinically relevant ST10 and ST58 were also identified. Extended-spectrum β-lactamase (ESBL) genes blaCTX–M–55 and blaCTX–M–8 predominated, often co-occurring with fluoroquinolone resistance genes qnrS1 and qnrB19. A total of 197 plasmids were identified; 63.6% were classified as conjugative. The most frequent relaxase types were MOBP (37 plasmids) and MOBF (24 plasmids), with IncI-gamma/K1 and IncF plasmids commonly linked to ESBL genes.DiscussionThis study provides one of the first genomic characterizations of plasmid-mediated AMR in poultry-associated E. coli in the Middle East. The high prevalence of MDR and mobile resistance elements underscores the role of international poultry trade in AMR dissemination. These findings highlight the need for strengthened AMR surveillance and improved regulatory control over antibiotic use in poultry production to mitigate public health risks.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590906/fullEscherichia coliantibiotic resistanceimported poultrywhole-genome sequencingUnited Arab Emirates
spellingShingle Ihab Habib
Ihab Habib
Mohamed-Yousif Ibrahim Mohamed
Mohamed-Yousif Ibrahim Mohamed
Glindya Bhagya Lakshmi
Glindya Bhagya Lakshmi
Akela Ghazawi
Akela Ghazawi
Mushtaq Khan
Mushtaq Khan
Hazim O. Khalifa
Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
Frontiers in Microbiology
Escherichia coli
antibiotic resistance
imported poultry
whole-genome sequencing
United Arab Emirates
title Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
title_full Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
title_fullStr Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
title_full_unstemmed Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
title_short Characterizing antimicrobial resistance and plasmidome diversity in Escherichia coli from imported frozen broiler chicken in the United Arab Emirates
title_sort characterizing antimicrobial resistance and plasmidome diversity in escherichia coli from imported frozen broiler chicken in the united arab emirates
topic Escherichia coli
antibiotic resistance
imported poultry
whole-genome sequencing
United Arab Emirates
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590906/full
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