Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study
Background: Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity. Objective: To investigate the Res impact on the catalase (Cat), glutathione peroxidase (Gpx), and superoxide dismutase (Sod) gene expression in the ovary and on the Cat and G...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2025-01-01
|
| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://knepublishing.com/index.php/ijrm/article/view/17821 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841527865798033408 |
|---|---|
| author | Mohammad Karimian Mozhdeh Ghadiri Seyedeh Mahsa Poormoosavi Hossein Najafzadehvarzi |
| author_facet | Mohammad Karimian Mozhdeh Ghadiri Seyedeh Mahsa Poormoosavi Hossein Najafzadehvarzi |
| author_sort | Mohammad Karimian |
| collection | DOAJ |
| description |
Background: Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity.
Objective: To investigate the Res impact on the catalase (Cat), glutathione peroxidase (Gpx), and superoxide dismutase (Sod) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate.
Materials and Methods: In this experimental study, 33 female Wistar rats (8–10 wk, 180–200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of Cat, Gpx, and Sod was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function.
Results: Lead acetate significantly downregulates Cat, Gpx, and Sod gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for Cat, 20 mg/kg and 40 mg/kg for Gpx, and 80 mg/kg for Sod. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes.
Conclusion: Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective.
|
| format | Article |
| id | doaj-art-ec1b42262d664c73a6839bd3f27da444 |
| institution | Kabale University |
| issn | 2476-4108 2476-3772 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj-art-ec1b42262d664c73a6839bd3f27da4442025-01-15T06:16:57ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722025-01-01221110.18502/ijrm.v22i11.17821Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental studyMohammad Karimian0Mozhdeh Ghadiri1Seyedeh Mahsa Poormoosavi2Hossein Najafzadehvarzi3Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, IranFaculty of Pharmacy, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran.Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Background: Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity. Objective: To investigate the Res impact on the catalase (Cat), glutathione peroxidase (Gpx), and superoxide dismutase (Sod) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate. Materials and Methods: In this experimental study, 33 female Wistar rats (8–10 wk, 180–200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of Cat, Gpx, and Sod was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function. Results: Lead acetate significantly downregulates Cat, Gpx, and Sod gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for Cat, 20 mg/kg and 40 mg/kg for Gpx, and 80 mg/kg for Sod. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes. Conclusion: Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective. https://knepublishing.com/index.php/ijrm/article/view/17821Resveratrol, Lead acetate, Oxidative stress, Ovary, In silico, Rat. |
| spellingShingle | Mohammad Karimian Mozhdeh Ghadiri Seyedeh Mahsa Poormoosavi Hossein Najafzadehvarzi Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study International Journal of Reproductive BioMedicine Resveratrol, Lead acetate, Oxidative stress, Ovary, In silico, Rat. |
| title | Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study |
| title_full | Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study |
| title_fullStr | Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study |
| title_full_unstemmed | Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study |
| title_short | Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study |
| title_sort | protective effects of resveratrol on the expression of catalase glutathione peroxidase and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate an experimental study |
| topic | Resveratrol, Lead acetate, Oxidative stress, Ovary, In silico, Rat. |
| url | https://knepublishing.com/index.php/ijrm/article/view/17821 |
| work_keys_str_mv | AT mohammadkarimian protectiveeffectsofresveratrolontheexpressionofcatalaseglutathioneperoxidaseandsuperoxidedismutasegenesintheovaryandtheiractivityintheserumofratsexposedtoleadacetateanexperimentalstudy AT mozhdehghadiri protectiveeffectsofresveratrolontheexpressionofcatalaseglutathioneperoxidaseandsuperoxidedismutasegenesintheovaryandtheiractivityintheserumofratsexposedtoleadacetateanexperimentalstudy AT seyedehmahsapoormoosavi protectiveeffectsofresveratrolontheexpressionofcatalaseglutathioneperoxidaseandsuperoxidedismutasegenesintheovaryandtheiractivityintheserumofratsexposedtoleadacetateanexperimentalstudy AT hosseinnajafzadehvarzi protectiveeffectsofresveratrolontheexpressionofcatalaseglutathioneperoxidaseandsuperoxidedismutasegenesintheovaryandtheiractivityintheserumofratsexposedtoleadacetateanexperimentalstudy |