Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by m...

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Main Authors: Mohsen Keyvanfard, Khadijeh Alizad, Razieh Shakeri
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2013/206784
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author Mohsen Keyvanfard
Khadijeh Alizad
Razieh Shakeri
author_facet Mohsen Keyvanfard
Khadijeh Alizad
Razieh Shakeri
author_sort Mohsen Keyvanfard
collection DOAJ
description A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1 SCG. The limit of detection is 2.7 ng mL−1 SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1 SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.
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publishDate 2013-01-01
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spelling doaj-art-ec182c3c9666476f837c83ae6314c8db2025-08-20T03:21:23ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/206784206784Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological SamplesMohsen Keyvanfard0Khadijeh Alizad1Razieh Shakeri2Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan 8631656451, IranDepartment of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan 8631656451, IranDepartment of Chemistry, Shahreza Branch, Islamic Azad University, Isfahan 8614773366, IranA new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1 SCG. The limit of detection is 2.7 ng mL−1 SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1 SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.http://dx.doi.org/10.1155/2013/206784
spellingShingle Mohsen Keyvanfard
Khadijeh Alizad
Razieh Shakeri
Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
Journal of Chemistry
title Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
title_full Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
title_fullStr Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
title_full_unstemmed Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
title_short Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples
title_sort determination of sodium cromoglycate by a new kinetic spectrophotometric method in biological samples
url http://dx.doi.org/10.1155/2013/206784
work_keys_str_mv AT mohsenkeyvanfard determinationofsodiumcromoglycatebyanewkineticspectrophotometricmethodinbiologicalsamples
AT khadijehalizad determinationofsodiumcromoglycatebyanewkineticspectrophotometricmethodinbiologicalsamples
AT raziehshakeri determinationofsodiumcromoglycatebyanewkineticspectrophotometricmethodinbiologicalsamples