Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine
Pilot studies of pharmacokinetics and pharmacodynamics of new photosensitizer liposomal boronated chlorine in mice with melanoma В16 were performed. Dynamics of drug accumulation in tumor and normal tissue (level of accumulation in tissues was assessed by means of fl uorescence) was studied. For int...
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| Format: | Article |
| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2015-06-01
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| Series: | Biomedical Photonics |
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| Online Access: | https://www.pdt-journal.com/jour/article/view/55 |
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| author | Yu. S. Osipchuk M. A. Kaplan V. V. Drozhzhina |
| author_facet | Yu. S. Osipchuk M. A. Kaplan V. V. Drozhzhina |
| author_sort | Yu. S. Osipchuk |
| collection | DOAJ |
| description | Pilot studies of pharmacokinetics and pharmacodynamics of new photosensitizer liposomal boronated chlorine in mice with melanoma В16 were performed. Dynamics of drug accumulation in tumor and normal tissue (level of accumulation in tissues was assessed by means of fl uorescence) was studied. For intraperitoneal injection at a dose of 5 mg/kg the drug was shown to accumulate gradually in tumor and normal tissues with maximal fl uorescence values in 2–3 h after injection. Then fl uorescence intensity in tissues gradually decreased, achieving nearly initial base-line in 120 h after beginning of the study. The hotosensitizer accumulated in tumor tissue with average selectivity: contrast index for maximal drug accumulation in tissues accounted for 1.7–2.8. According to obtained data for following research the time interval between drug injection and irradiation of 3h was chosen. On the second stage of the study dependence of photodynamic therapy effi cacy on different parameters including photosensitizer dose for intraperitoneal injection (5–10 mg/kg), light dose (150–300 J/cm2) and power density (0.25–0.51 mW/cm2). The irradiation was performed by semiconductor laser on wavelength of 662±1 nm. The effi cacy was maximal for following regimen: photosensitizer dose of 5 mg/kg, light dose of 300 J/cm2, power intensity of 0.44 mW/cm2. For this regimen at the end of follow-up (21 days) complete tumor regression was obtained in 75% of animals and average coeffi cient of absolute tumor growth accounted for only 7.82. |
| format | Article |
| id | doaj-art-ec0a18623de349a9bf3d2446341ecb0f |
| institution | Kabale University |
| issn | 2413-9432 |
| language | Russian |
| publishDate | 2015-06-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj-art-ec0a18623de349a9bf3d2446341ecb0f2025-08-20T03:35:29ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322015-06-01423810.24931/2413-9432-2015-4-2-3-853Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorineYu. S. Osipchuk0M. A. Kaplan1V. V. Drozhzhina2A.F.Tsyb MRRC – branch of FSI « NMRRC» of Russian Ministry of Health, ObninskA.F.Tsyb MRRC – branch of FSI « NMRRC» of Russian Ministry of Health, ObninskA.F.Tsyb MRRC – branch of FSI « NMRRC» of Russian Ministry of Health, ObninskPilot studies of pharmacokinetics and pharmacodynamics of new photosensitizer liposomal boronated chlorine in mice with melanoma В16 were performed. Dynamics of drug accumulation in tumor and normal tissue (level of accumulation in tissues was assessed by means of fl uorescence) was studied. For intraperitoneal injection at a dose of 5 mg/kg the drug was shown to accumulate gradually in tumor and normal tissues with maximal fl uorescence values in 2–3 h after injection. Then fl uorescence intensity in tissues gradually decreased, achieving nearly initial base-line in 120 h after beginning of the study. The hotosensitizer accumulated in tumor tissue with average selectivity: contrast index for maximal drug accumulation in tissues accounted for 1.7–2.8. According to obtained data for following research the time interval between drug injection and irradiation of 3h was chosen. On the second stage of the study dependence of photodynamic therapy effi cacy on different parameters including photosensitizer dose for intraperitoneal injection (5–10 mg/kg), light dose (150–300 J/cm2) and power density (0.25–0.51 mW/cm2). The irradiation was performed by semiconductor laser on wavelength of 662±1 nm. The effi cacy was maximal for following regimen: photosensitizer dose of 5 mg/kg, light dose of 300 J/cm2, power intensity of 0.44 mW/cm2. For this regimen at the end of follow-up (21 days) complete tumor regression was obtained in 75% of animals and average coeffi cient of absolute tumor growth accounted for only 7.82.https://www.pdt-journal.com/jour/article/view/55photodynamic therapyfotogemphotosensalasensfotoditazinradachlorine |
| spellingShingle | Yu. S. Osipchuk M. A. Kaplan V. V. Drozhzhina Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine Biomedical Photonics photodynamic therapy fotogem photosens alasens fotoditazin radachlorine |
| title | Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine |
| title_full | Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine |
| title_fullStr | Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine |
| title_full_unstemmed | Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine |
| title_short | Photodynamic therapy of melanoma B16 in mice with the new photosensitizer boronated chlorine |
| title_sort | photodynamic therapy of melanoma b16 in mice with the new photosensitizer boronated chlorine |
| topic | photodynamic therapy fotogem photosens alasens fotoditazin radachlorine |
| url | https://www.pdt-journal.com/jour/article/view/55 |
| work_keys_str_mv | AT yusosipchuk photodynamictherapyofmelanomab16inmicewiththenewphotosensitizerboronatedchlorine AT makaplan photodynamictherapyofmelanomab16inmicewiththenewphotosensitizerboronatedchlorine AT vvdrozhzhina photodynamictherapyofmelanomab16inmicewiththenewphotosensitizerboronatedchlorine |