Mitochondrial membrane potential probes and the proton gradient: a practical usage guide
Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires caref...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2011-02-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000113610 |
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| author | Seth W. Perry John P. Norman Justin Barbieri Edward B. Brown Harris A. Gelbard |
| author_facet | Seth W. Perry John P. Norman Justin Barbieri Edward B. Brown Harris A. Gelbard |
| author_sort | Seth W. Perry |
| collection | DOAJ |
| description | Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research. |
| format | Article |
| id | doaj-art-ec051fb7e148470aacb792163e1fae1d |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2011-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ec051fb7e148470aacb792163e1fae1d2025-08-20T02:26:02ZengTaylor & Francis GroupBioTechniques0736-62051940-98182011-02-015029811510.2144/000113610Mitochondrial membrane potential probes and the proton gradient: a practical usage guideSeth W. Perry0John P. Norman1Justin Barbieri2Edward B. Brown3Harris A. Gelbard41Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA3Graduate Program in Toxicology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA4Center for Neural Development and Disease, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA1Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA2Department of Neurology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USAFluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.https://www.future-science.com/doi/10.2144/000113610Mitochondriahyperpolarizationdepolarizationmembrane potentialcalciumproton |
| spellingShingle | Seth W. Perry John P. Norman Justin Barbieri Edward B. Brown Harris A. Gelbard Mitochondrial membrane potential probes and the proton gradient: a practical usage guide BioTechniques Mitochondria hyperpolarization depolarization membrane potential calcium proton |
| title | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide |
| title_full | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide |
| title_fullStr | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide |
| title_full_unstemmed | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide |
| title_short | Mitochondrial membrane potential probes and the proton gradient: a practical usage guide |
| title_sort | mitochondrial membrane potential probes and the proton gradient a practical usage guide |
| topic | Mitochondria hyperpolarization depolarization membrane potential calcium proton |
| url | https://www.future-science.com/doi/10.2144/000113610 |
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