Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023
The eastern parts of Ethiopia, including Dire Dawa City, have experienced annual dengue fever (DF) outbreaks since 2013, leading to significant healthcare and economic impacts. However, comprehensive evidence on the specific dengue virus (DENV) serotypes and genotypes involved remains limited. Durin...
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2025-02-01
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| author | Abebe Aseffa Negeri Dawit Hailu Alemayehu Saro Abdella Abrahim Tsigereda Kifle Wolde Gutema Bulti Tura Alemnesh Hailemariam Bedasso Danile Tsega Geretsion Ebise Abose Djirata Eyilachew Zenebe Awule Diana Rojas-Gallardo Asefa Konde Korkiso Kalkidan Melaku Raffael Joseph Abaysew Ayele Mesfin Mengesha Tsegaye Anne Piantadosi Getachew Tollera Alemseged Abdissa Mesay Hailu Dangiso Adane Mihret Andargachew Mulu Tesfaye Gelanew |
| author_facet | Abebe Aseffa Negeri Dawit Hailu Alemayehu Saro Abdella Abrahim Tsigereda Kifle Wolde Gutema Bulti Tura Alemnesh Hailemariam Bedasso Danile Tsega Geretsion Ebise Abose Djirata Eyilachew Zenebe Awule Diana Rojas-Gallardo Asefa Konde Korkiso Kalkidan Melaku Raffael Joseph Abaysew Ayele Mesfin Mengesha Tsegaye Anne Piantadosi Getachew Tollera Alemseged Abdissa Mesay Hailu Dangiso Adane Mihret Andargachew Mulu Tesfaye Gelanew |
| author_sort | Abebe Aseffa Negeri |
| collection | DOAJ |
| description | The eastern parts of Ethiopia, including Dire Dawa City, have experienced annual dengue fever (DF) outbreaks since 2013, leading to significant healthcare and economic impacts. However, comprehensive evidence on the specific dengue virus (DENV) serotypes and genotypes involved remains limited. During the 2023 DF outbreak, the National Arbovirus Laboratory received seventy serum samples from suspected DF patients. Positive samples underwent sequencing of the CprM region of the DENV genome, and the obtained sequences were analyzed phylogenetically. Among the patients, 32 (45.7%) displayed early warning signs of severe dengue, and 13 were hospitalized, most showing symptoms indicative of severe dengue. Out of 67 adequate samples, 44 (65.6%) tested positive for DENV RNA by RT-PCR, and 17 successfully underwent CprM sequencing. All sequenced samples were identified as DENV-3, genotype III, major lineage B (DENV-3III_B), with two distinct minor lineages (DENV-3III_B.2 and DENV-3III_B.3). Phylogenetic analysis showed that these lineages were closely related to sequences from the Afar region, suggesting interconnected outbreaks with multiple co-circulating lineages. This study identifies DENV-3III_B as the cause of the 2023 DF outbreak in Dire Dawa City and highlights the need for enhanced viral genomic surveillance in Africa. |
| format | Article |
| id | doaj-art-ec0209a90abe44c6a1b91028abb2e9f4 |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Viruses |
| spelling | doaj-art-ec0209a90abe44c6a1b91028abb2e9f42025-08-20T02:43:05ZengMDPI AGViruses1999-49152025-02-0117334610.3390/v17030346Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023Abebe Aseffa Negeri0Dawit Hailu Alemayehu1Saro Abdella Abrahim2Tsigereda Kifle Wolde3Gutema Bulti Tura4Alemnesh Hailemariam Bedasso5Danile Tsega Geretsion6Ebise Abose Djirata7Eyilachew Zenebe Awule8Diana Rojas-Gallardo9Asefa Konde Korkiso10Kalkidan Melaku11Raffael Joseph12Abaysew Ayele13Mesfin Mengesha Tsegaye14Anne Piantadosi15Getachew Tollera16Alemseged Abdissa17Mesay Hailu Dangiso18Adane Mihret19Andargachew Mulu20Tesfaye Gelanew21Ethiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaDire Dawa City Administration Health Bureau, Dire Dawa P.O. Box 1377, EthiopiaPopulation Biology, Ecology and Evolution Graduate Program, Emory University, Atlanta, GA 30322, USAEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaDepartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USAEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, EthiopiaThe eastern parts of Ethiopia, including Dire Dawa City, have experienced annual dengue fever (DF) outbreaks since 2013, leading to significant healthcare and economic impacts. However, comprehensive evidence on the specific dengue virus (DENV) serotypes and genotypes involved remains limited. During the 2023 DF outbreak, the National Arbovirus Laboratory received seventy serum samples from suspected DF patients. Positive samples underwent sequencing of the CprM region of the DENV genome, and the obtained sequences were analyzed phylogenetically. Among the patients, 32 (45.7%) displayed early warning signs of severe dengue, and 13 were hospitalized, most showing symptoms indicative of severe dengue. Out of 67 adequate samples, 44 (65.6%) tested positive for DENV RNA by RT-PCR, and 17 successfully underwent CprM sequencing. All sequenced samples were identified as DENV-3, genotype III, major lineage B (DENV-3III_B), with two distinct minor lineages (DENV-3III_B.2 and DENV-3III_B.3). Phylogenetic analysis showed that these lineages were closely related to sequences from the Afar region, suggesting interconnected outbreaks with multiple co-circulating lineages. This study identifies DENV-3III_B as the cause of the 2023 DF outbreak in Dire Dawa City and highlights the need for enhanced viral genomic surveillance in Africa.https://www.mdpi.com/1999-4915/17/3/346DENVDFserotypegenotypeCprMDENV-3/GIII |
| spellingShingle | Abebe Aseffa Negeri Dawit Hailu Alemayehu Saro Abdella Abrahim Tsigereda Kifle Wolde Gutema Bulti Tura Alemnesh Hailemariam Bedasso Danile Tsega Geretsion Ebise Abose Djirata Eyilachew Zenebe Awule Diana Rojas-Gallardo Asefa Konde Korkiso Kalkidan Melaku Raffael Joseph Abaysew Ayele Mesfin Mengesha Tsegaye Anne Piantadosi Getachew Tollera Alemseged Abdissa Mesay Hailu Dangiso Adane Mihret Andargachew Mulu Tesfaye Gelanew Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 Viruses DENV DF serotype genotype CprM DENV-3/GIII |
| title | Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 |
| title_full | Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 |
| title_fullStr | Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 |
| title_full_unstemmed | Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 |
| title_short | Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023 |
| title_sort | lineage b genotype iii of dengue virus serotype 3 denv 3iii b is responsible for dengue outbreak in dire dawa city ethiopia 2023 |
| topic | DENV DF serotype genotype CprM DENV-3/GIII |
| url | https://www.mdpi.com/1999-4915/17/3/346 |
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