3D imaging of the human temporal bone by X-ray phase-contrast tomography
Abstract Studying the subtle and intricate three-dimensional structure of the human cochlea embedded in the temporal bone requires structure-preserving imaging approaches with adaptable field of view and resolution. Synchrotron X-ray phase-contrast tomography at the novel beamline BM18 (EBS, ESRF) o...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | npj Imaging |
| Online Access: | https://doi.org/10.1038/s44303-025-00086-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730929286709248 |
|---|---|
| author | Jannis J. Schaeper Paul Tafforeau Christoph A. Kampshoff Carolina Thomas Alexander Meyer Christine Stadelmann M. Charles Liberman Tobias Moser Tim Salditt |
| author_facet | Jannis J. Schaeper Paul Tafforeau Christoph A. Kampshoff Carolina Thomas Alexander Meyer Christine Stadelmann M. Charles Liberman Tobias Moser Tim Salditt |
| author_sort | Jannis J. Schaeper |
| collection | DOAJ |
| description | Abstract Studying the subtle and intricate three-dimensional structure of the human cochlea embedded in the temporal bone requires structure-preserving imaging approaches with adaptable field of view and resolution. Synchrotron X-ray phase-contrast tomography at the novel beamline BM18 (EBS, ESRF) offers the unique capability to achieve histological resolution at the scale of the entire organ, based on high lateral coherence, long propagation distances, and optimized spectral range. At the same time advances in laboratory μ-CT instrumentation and protocols also open up new opportunities for 3D micro-anatomy and histopathology, including 3D reconstruction of nerve tissue when suitable staining protocols are used. Here we report on post mortem 3D imaging of human temporal bones and excised human cochleae, both unstained and stained to visualize the auditory nerve. Further, we highlight the use of this imaging modality for development of novel cochlear implant technology. |
| format | Article |
| id | doaj-art-ec005e6acf0246b19ff5130fd8c0d983 |
| institution | DOAJ |
| issn | 2948-197X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Imaging |
| spelling | doaj-art-ec005e6acf0246b19ff5130fd8c0d9832025-08-20T03:08:43ZengNature Portfolionpj Imaging2948-197X2025-05-013111110.1038/s44303-025-00086-y3D imaging of the human temporal bone by X-ray phase-contrast tomographyJannis J. Schaeper0Paul Tafforeau1Christoph A. Kampshoff2Carolina Thomas3Alexander Meyer4Christine Stadelmann5M. Charles Liberman6Tobias Moser7Tim Salditt8Institute for X-ray Physics, University of GöttingenBeamline BM18, European Synchrotron Radiation FacilityInstitute for Auditory Neuroscience, University Medical Center GöttingenCluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells”, University of GöttingenDepartment of Otolaryngology, University Medical Center GöttingenCluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells”, University of GöttingenEaton-Peabody Laboratories, Massachusetts Eye and Ear InfirmaryCluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells”, University of GöttingenInstitute for X-ray Physics, University of GöttingenAbstract Studying the subtle and intricate three-dimensional structure of the human cochlea embedded in the temporal bone requires structure-preserving imaging approaches with adaptable field of view and resolution. Synchrotron X-ray phase-contrast tomography at the novel beamline BM18 (EBS, ESRF) offers the unique capability to achieve histological resolution at the scale of the entire organ, based on high lateral coherence, long propagation distances, and optimized spectral range. At the same time advances in laboratory μ-CT instrumentation and protocols also open up new opportunities for 3D micro-anatomy and histopathology, including 3D reconstruction of nerve tissue when suitable staining protocols are used. Here we report on post mortem 3D imaging of human temporal bones and excised human cochleae, both unstained and stained to visualize the auditory nerve. Further, we highlight the use of this imaging modality for development of novel cochlear implant technology.https://doi.org/10.1038/s44303-025-00086-y |
| spellingShingle | Jannis J. Schaeper Paul Tafforeau Christoph A. Kampshoff Carolina Thomas Alexander Meyer Christine Stadelmann M. Charles Liberman Tobias Moser Tim Salditt 3D imaging of the human temporal bone by X-ray phase-contrast tomography npj Imaging |
| title | 3D imaging of the human temporal bone by X-ray phase-contrast tomography |
| title_full | 3D imaging of the human temporal bone by X-ray phase-contrast tomography |
| title_fullStr | 3D imaging of the human temporal bone by X-ray phase-contrast tomography |
| title_full_unstemmed | 3D imaging of the human temporal bone by X-ray phase-contrast tomography |
| title_short | 3D imaging of the human temporal bone by X-ray phase-contrast tomography |
| title_sort | 3d imaging of the human temporal bone by x ray phase contrast tomography |
| url | https://doi.org/10.1038/s44303-025-00086-y |
| work_keys_str_mv | AT jannisjschaeper 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT paultafforeau 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT christophakampshoff 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT carolinathomas 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT alexandermeyer 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT christinestadelmann 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT mcharlesliberman 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT tobiasmoser 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography AT timsalditt 3dimagingofthehumantemporalbonebyxrayphasecontrasttomography |