Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2006.265 |
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| author | Tetsuji Nagata |
| author_facet | Tetsuji Nagata |
| author_sort | Tetsuji Nagata |
| collection | DOAJ |
| description | For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic (EM) radioautography. On EM radioautograms obtained from each animal, the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with silver grains due to 3H-leucine showing protein synthesis in each mononucleate hepatocytes were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in mononucleate hepatocytes of mice at various ages from embryonic day 19 to postnatal month 24 increased and decreased due to development and aging of animals. |
| format | Article |
| id | doaj-art-ebfe71a0b3664b37b4f944470e12466e |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ebfe71a0b3664b37b4f944470e12466e2025-08-20T02:21:46ZengWileyThe Scientific World Journal1537-744X2006-01-0161583159810.1100/tsw.2006.265Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging MiceTetsuji Nagata0Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto 390-8621 and Department of Anatomy, Shinshu Institute of Alternative Medicine, Nagano 380-0816, JapanFor the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic (EM) radioautography. On EM radioautograms obtained from each animal, the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with silver grains due to 3H-leucine showing protein synthesis in each mononucleate hepatocytes were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in mononucleate hepatocytes of mice at various ages from embryonic day 19 to postnatal month 24 increased and decreased due to development and aging of animals.http://dx.doi.org/10.1100/tsw.2006.265 |
| spellingShingle | Tetsuji Nagata Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice The Scientific World Journal |
| title | Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice |
| title_full | Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice |
| title_fullStr | Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice |
| title_full_unstemmed | Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice |
| title_short | Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice |
| title_sort | electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of aging mice |
| url | http://dx.doi.org/10.1100/tsw.2006.265 |
| work_keys_str_mv | AT tetsujinagata electronmicroscopicradioautographicstudyonproteinsynthesisinhepatocytemitochondriaofagingmice |