Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1
Soluble programmed death ligand‐1 (sPD‐L1), a pivotal immune checkpoint protein, serves as a biomarker for evaluating the efficacy of cancer therapies. Aptamers, as highly stable and specific recognition elements, play an essential role in emerging point‐of‐care diagnostic technologies. Yet, crucial...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Small Science |
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| Online Access: | https://doi.org/10.1002/smsc.202400411 |
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| author | Zahra Lotfibakalani Borui Liu Monalisha Ghosh Dastidar Thành Trân‐Phú Krishnan Murugappan Parisa Moazzam David R Nisbet Antonio Tricoli |
| author_facet | Zahra Lotfibakalani Borui Liu Monalisha Ghosh Dastidar Thành Trân‐Phú Krishnan Murugappan Parisa Moazzam David R Nisbet Antonio Tricoli |
| author_sort | Zahra Lotfibakalani |
| collection | DOAJ |
| description | Soluble programmed death ligand‐1 (sPD‐L1), a pivotal immune checkpoint protein, serves as a biomarker for evaluating the efficacy of cancer therapies. Aptamers, as highly stable and specific recognition elements, play an essential role in emerging point‐of‐care diagnostic technologies. Yet, crucial advancements rely on engineering the intricate interaction between aptamers and sensor substrates to achieve specificity and signal enhancement. Here, a comprehensive physicochemical characterization and performance optimization of a sPD‐L1 aptamer‐based biosensor by a complementary set of state‐of‐the‐art methodologies is presented, including atomic force microscopy‐based infrared spectroscopy and high‐resolution transmission electron microscopy, providing critical insights on the surface coverage and binding mechanism. The optimal nanoaptasensors detect sPD‐L1 across a wide concentration range (from am to μm) with a detection limit of 0.76 am in both buffer and mouse serum samples. These findings, demonstrating superior selectivity, reproducibility, and stability, pave the way for engineering miniaturized point‐of‐care and portable biosensors for cancer diagnostics. |
| format | Article |
| id | doaj-art-ebf5eb143cc743b48b8322d6ca4b36b6 |
| institution | DOAJ |
| issn | 2688-4046 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-ebf5eb143cc743b48b8322d6ca4b36b62025-08-20T02:39:58ZengWiley-VCHSmall Science2688-40462025-01-0151n/an/a10.1002/smsc.202400411Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1Zahra Lotfibakalani0Borui Liu1Monalisha Ghosh Dastidar2Thành Trân‐Phú3Krishnan Murugappan4Parisa Moazzam5David R Nisbet6Antonio Tricoli7Nanotechnology Research Laboratory Faculty of Engineering The University of Sydney Darlington NSW 2008 AustraliaNanotechnology Research Laboratory Faculty of Engineering The University of Sydney Darlington NSW 2008 AustraliaNanotechnology Research Laboratory Research School of Chemistry Australian National University Canberra ACT 2601 AustraliaNanotechnology Research Laboratory Research School of Chemistry Australian National University Canberra ACT 2601 AustraliaNanotechnology Research Laboratory Research School of Chemistry Australian National University Canberra ACT 2601 AustraliaNanotechnology Research Laboratory Faculty of Engineering The University of Sydney Darlington NSW 2008 AustraliaThe Graeme Clark Institute The University of Melbourne Melbourne VIC 3010 AustraliaNanotechnology Research Laboratory Faculty of Engineering The University of Sydney Darlington NSW 2008 AustraliaSoluble programmed death ligand‐1 (sPD‐L1), a pivotal immune checkpoint protein, serves as a biomarker for evaluating the efficacy of cancer therapies. Aptamers, as highly stable and specific recognition elements, play an essential role in emerging point‐of‐care diagnostic technologies. Yet, crucial advancements rely on engineering the intricate interaction between aptamers and sensor substrates to achieve specificity and signal enhancement. Here, a comprehensive physicochemical characterization and performance optimization of a sPD‐L1 aptamer‐based biosensor by a complementary set of state‐of‐the‐art methodologies is presented, including atomic force microscopy‐based infrared spectroscopy and high‐resolution transmission electron microscopy, providing critical insights on the surface coverage and binding mechanism. The optimal nanoaptasensors detect sPD‐L1 across a wide concentration range (from am to μm) with a detection limit of 0.76 am in both buffer and mouse serum samples. These findings, demonstrating superior selectivity, reproducibility, and stability, pave the way for engineering miniaturized point‐of‐care and portable biosensors for cancer diagnostics.https://doi.org/10.1002/smsc.202400411aptasensorselectrochemical detectionsgold nanoislandspoint‐of‐care biosensorssoluble programmed death ligand‐1 |
| spellingShingle | Zahra Lotfibakalani Borui Liu Monalisha Ghosh Dastidar Thành Trân‐Phú Krishnan Murugappan Parisa Moazzam David R Nisbet Antonio Tricoli Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 Small Science aptasensors electrochemical detections gold nanoislands point‐of‐care biosensors soluble programmed death ligand‐1 |
| title | Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 |
| title_full | Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 |
| title_fullStr | Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 |
| title_full_unstemmed | Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 |
| title_short | Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1 |
| title_sort | optimal biofunctionalization of gold nanoislands for electrochemical detection of soluble programmed death ligand 1 |
| topic | aptasensors electrochemical detections gold nanoislands point‐of‐care biosensors soluble programmed death ligand‐1 |
| url | https://doi.org/10.1002/smsc.202400411 |
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