Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet

Brazilian Rainforest is the habitat of many species of the genus Brachycephalus, among them is the pumpkin toadlet, Brachycephalus ephippium. X-ray microcomputed tomography is a nondestructive imaging technique which allows the visualization and analysis of internal microstructures of various sample...

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Main Authors: Caio Sorrentino dos Santos, Olga Oliveira Araujo, Hugo da Costa Romberg Júnior, Tâmara Porfíro Teixeira, Luan Ferreira Bastos, Alessandra Silveira Machado, Davi Ferreira Oliveira, Sérgio Furtado dos Reis, Ricardo Tadeu Lopes
Format: Article
Language:English
Published: Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR) 2021-04-01
Series:Brazilian Journal of Radiation Sciences
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Online Access:https://bjrs.org.br/revista/index.php/REVISTA/article/view/1548
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author Caio Sorrentino dos Santos
Olga Oliveira Araujo
Hugo da Costa Romberg Júnior
Tâmara Porfíro Teixeira
Luan Ferreira Bastos
Alessandra Silveira Machado
Davi Ferreira Oliveira
Sérgio Furtado dos Reis
Ricardo Tadeu Lopes
author_facet Caio Sorrentino dos Santos
Olga Oliveira Araujo
Hugo da Costa Romberg Júnior
Tâmara Porfíro Teixeira
Luan Ferreira Bastos
Alessandra Silveira Machado
Davi Ferreira Oliveira
Sérgio Furtado dos Reis
Ricardo Tadeu Lopes
author_sort Caio Sorrentino dos Santos
collection DOAJ
description Brazilian Rainforest is the habitat of many species of the genus Brachycephalus, among them is the pumpkin toadlet, Brachycephalus ephippium. X-ray microcomputed tomography is a nondestructive imaging technique which allows the visualization and analysis of internal microstructures of various samples, and has been applied on the study of these animals, enabling thorough description and characterization of new species. Brachycephalus ephippium is of particular interest for it was the first Brachycephalus species to be discovered, which makes it a common interspecies comparative basis. Cranial morphological landmarks used in biological research are very small (0.1-1mm), hence the effects of voxel size on microCT images can be relevant, and this is what was investigated in this work. Effects of reconstruction voxel size were evaluated in both visual and quantitative perspectives, which showed that an intermediary voxel size could be chosen on similar microCT applications without significant loss of information, but with great processing and storage gain, optimizing the application of the technique in such works.
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publisher Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)
record_format Article
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spelling doaj-art-ebea1a00f68945da846f94fefdd871032025-08-20T03:13:32ZengBrazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)Brazilian Journal of Radiation Sciences2319-06122021-04-0191A10.15392/bjrs.v9i1A.15481202Impact of voxel size on microCT morphometric analysis of the pumpkin toadletCaio Sorrentino dos Santos0Olga Oliveira Araujo1Hugo da Costa Romberg Júnior2Tâmara Porfíro Teixeira3Luan Ferreira Bastos4Alessandra Silveira Machado5Davi Ferreira Oliveira6Sérgio Furtado dos Reis7Ricardo Tadeu Lopes8Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal do Rio de Janeiro (UFRJ)State University of Campinas (UNICAMP)Universidade Federal do Rio de Janeiro (UFRJ)Brazilian Rainforest is the habitat of many species of the genus Brachycephalus, among them is the pumpkin toadlet, Brachycephalus ephippium. X-ray microcomputed tomography is a nondestructive imaging technique which allows the visualization and analysis of internal microstructures of various samples, and has been applied on the study of these animals, enabling thorough description and characterization of new species. Brachycephalus ephippium is of particular interest for it was the first Brachycephalus species to be discovered, which makes it a common interspecies comparative basis. Cranial morphological landmarks used in biological research are very small (0.1-1mm), hence the effects of voxel size on microCT images can be relevant, and this is what was investigated in this work. Effects of reconstruction voxel size were evaluated in both visual and quantitative perspectives, which showed that an intermediary voxel size could be chosen on similar microCT applications without significant loss of information, but with great processing and storage gain, optimizing the application of the technique in such works.https://bjrs.org.br/revista/index.php/REVISTA/article/view/1548microctsegmentationbrachycephalusvoxel sizereconstruction
spellingShingle Caio Sorrentino dos Santos
Olga Oliveira Araujo
Hugo da Costa Romberg Júnior
Tâmara Porfíro Teixeira
Luan Ferreira Bastos
Alessandra Silveira Machado
Davi Ferreira Oliveira
Sérgio Furtado dos Reis
Ricardo Tadeu Lopes
Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
Brazilian Journal of Radiation Sciences
microct
segmentation
brachycephalus
voxel size
reconstruction
title Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
title_full Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
title_fullStr Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
title_full_unstemmed Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
title_short Impact of voxel size on microCT morphometric analysis of the pumpkin toadlet
title_sort impact of voxel size on microct morphometric analysis of the pumpkin toadlet
topic microct
segmentation
brachycephalus
voxel size
reconstruction
url https://bjrs.org.br/revista/index.php/REVISTA/article/view/1548
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