Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species
Fructose-1,6-bisphosphatase (FBP) is a crucial regulatory enzyme in sucrose synthesis and photosynthetic carbon assimilation, functioning through two distinct isoforms: cytosolic FBP (cyFBP) and chloroplastic FBP (cpFBP). However, the identification and functional characterization of <i>FBP<...
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2025-08-01
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| author | Chunyan Tian Xiuting Hua Peifang Zhao Chunjia Li Xujuan Li Hongbo Liu Xinlong Liu |
| author_facet | Chunyan Tian Xiuting Hua Peifang Zhao Chunjia Li Xujuan Li Hongbo Liu Xinlong Liu |
| author_sort | Chunyan Tian |
| collection | DOAJ |
| description | Fructose-1,6-bisphosphatase (FBP) is a crucial regulatory enzyme in sucrose synthesis and photosynthetic carbon assimilation, functioning through two distinct isoforms: cytosolic FBP (cyFBP) and chloroplastic FBP (cpFBP). However, the identification and functional characterization of <i>FBP</i> genes in <i>Saccharum</i> remains limited. In this study, we conducted a systematic identification and comparative genomics analyses of <i>FBPs</i> in three <i>Saccharum</i> species. We further examined their expression patterns across leaf developmental zones, spatiotemporal profiles, and responses to diurnal rhythms and hormonal treatments. Our analysis identified 95 <i>FBP</i> genes, including 44 <i>cyFBPs</i> and 51 <i>cpFBPs</i>. Comparative analyses revealed significant divergence in physicochemical properties, gene structures, and motif compositions between the two isoforms. Expression profiling indicated that both <i>cyFBPs</i> and <i>cpFBPs</i> were predominantly expressed in leaves, particularly in maturing and mature zones. During diurnal cycles, their expression peaked around the night–day transition, with <i>cpFBPs</i> exhibiting earlier peaks than <i>cyFBPs</i>. <i>FBP genes</i> in <i>Saccharum spontaneum</i> displayed greater diurnal sensitivity than those in <i>Saccharum officinarum</i>. Hormonal treatments further revealed significant regulatory divergence in <i>FBP</i> genes, both between isoforms and across species. Notably, <i>cyFBP</i>_<i>2</i> and <i>cpFBP</i>_<i>2</i> members consistently exhibited higher expression levels across all datasets, suggesting their pivotal roles in sugarcane physiology. These findings not only identify potential target genes for enhancing sucrose accumulation, but also highlight the breeding value of <i>S. spontaneum</i> and <i>S. officinarum</i> in sugarcane breeding. |
| format | Article |
| id | doaj-art-ebe2ec64aa844855a14c6f5025b66c2d |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-ebe2ec64aa844855a14c6f5025b66c2d2025-08-20T03:02:56ZengMDPI AGPlants2223-77472025-08-011415243310.3390/plants14152433Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> SpeciesChunyan Tian0Xiuting Hua1Peifang Zhao2Chunjia Li3Xujuan Li4Hongbo Liu5Xinlong Liu6State Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning 530004, ChinaState Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaState Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaState Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaState Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaState Key Laboratory for Tropical Crop Breeding, Kunming 650205, ChinaFructose-1,6-bisphosphatase (FBP) is a crucial regulatory enzyme in sucrose synthesis and photosynthetic carbon assimilation, functioning through two distinct isoforms: cytosolic FBP (cyFBP) and chloroplastic FBP (cpFBP). However, the identification and functional characterization of <i>FBP</i> genes in <i>Saccharum</i> remains limited. In this study, we conducted a systematic identification and comparative genomics analyses of <i>FBPs</i> in three <i>Saccharum</i> species. We further examined their expression patterns across leaf developmental zones, spatiotemporal profiles, and responses to diurnal rhythms and hormonal treatments. Our analysis identified 95 <i>FBP</i> genes, including 44 <i>cyFBPs</i> and 51 <i>cpFBPs</i>. Comparative analyses revealed significant divergence in physicochemical properties, gene structures, and motif compositions between the two isoforms. Expression profiling indicated that both <i>cyFBPs</i> and <i>cpFBPs</i> were predominantly expressed in leaves, particularly in maturing and mature zones. During diurnal cycles, their expression peaked around the night–day transition, with <i>cpFBPs</i> exhibiting earlier peaks than <i>cyFBPs</i>. <i>FBP genes</i> in <i>Saccharum spontaneum</i> displayed greater diurnal sensitivity than those in <i>Saccharum officinarum</i>. Hormonal treatments further revealed significant regulatory divergence in <i>FBP</i> genes, both between isoforms and across species. Notably, <i>cyFBP</i>_<i>2</i> and <i>cpFBP</i>_<i>2</i> members consistently exhibited higher expression levels across all datasets, suggesting their pivotal roles in sugarcane physiology. These findings not only identify potential target genes for enhancing sucrose accumulation, but also highlight the breeding value of <i>S. spontaneum</i> and <i>S. officinarum</i> in sugarcane breeding.https://www.mdpi.com/2223-7747/14/15/2433<i>FBP</i> gene familygenome-wide identificationexpression patternsfunctional divergence<i>Saccharum spontaneum</i><i>Saccharum officinarum</i> |
| spellingShingle | Chunyan Tian Xiuting Hua Peifang Zhao Chunjia Li Xujuan Li Hongbo Liu Xinlong Liu Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species Plants <i>FBP</i> gene family genome-wide identification expression patterns functional divergence <i>Saccharum spontaneum</i> <i>Saccharum officinarum</i> |
| title | Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species |
| title_full | Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species |
| title_fullStr | Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species |
| title_full_unstemmed | Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species |
| title_short | Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family in <i>Saccharum</i> Species |
| title_sort | genome wide identification evolution and expression patterns of the fructose 1 6 bisphosphatase gene family in i saccharum i species |
| topic | <i>FBP</i> gene family genome-wide identification expression patterns functional divergence <i>Saccharum spontaneum</i> <i>Saccharum officinarum</i> |
| url | https://www.mdpi.com/2223-7747/14/15/2433 |
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