Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19

Abstract Background Although several genetic biomarkers have been reported in the tocilizumab (TCZ) response in rheumatoid arthritis, no studies have addressed the pharmacogenomics effect of TCZ in COVID-19. Methods In this prospective longitudinal study, 95 individuals with severe COVID-19 were sel...

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Main Authors: Nastaran Injinari, Samira Asadollahi, Fateme Sefid, Maedeh Arshadi, Saeedeh Sadat Hosseini, Hamed Ghoshouni, Fatemeh Soltani, Nasim Namiranian, Mohammad Hasan Sheikhha, Fatemeh Aghaeimeybodi
Format: Article
Language:English
Published: BMC 2024-10-01
Series:BMC Infectious Diseases
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Online Access:https://doi.org/10.1186/s12879-024-10073-0
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author Nastaran Injinari
Samira Asadollahi
Fateme Sefid
Maedeh Arshadi
Saeedeh Sadat Hosseini
Hamed Ghoshouni
Fatemeh Soltani
Nasim Namiranian
Mohammad Hasan Sheikhha
Fatemeh Aghaeimeybodi
author_facet Nastaran Injinari
Samira Asadollahi
Fateme Sefid
Maedeh Arshadi
Saeedeh Sadat Hosseini
Hamed Ghoshouni
Fatemeh Soltani
Nasim Namiranian
Mohammad Hasan Sheikhha
Fatemeh Aghaeimeybodi
author_sort Nastaran Injinari
collection DOAJ
description Abstract Background Although several genetic biomarkers have been reported in the tocilizumab (TCZ) response in rheumatoid arthritis, no studies have addressed the pharmacogenomics effect of TCZ in COVID-19. Methods In this prospective longitudinal study, 95 individuals with severe COVID-19 were selected between 2020–2022. The recovery process was measured at 24 h, 48 h, and 10 days before and after taking TCZ. All participants were genotyped using RFLP-PCR. Different genotypes of FCGR2A rs1801274 and IL-6R rs2228145 were compared in terms of the recovery process. Results 43.2% of patients were male and 56.8% were female with an average age of 58.20(± 16.214) years. The GA genotype for FCGR2A rs1801274 increased the risk of death (OR = 2.83, P = 0.038) and ventilation (OR = 2.71, P = 0.047) in TCZ-treated individuals. However, there was no risk of death and ventilation with IL-6R rs2228145 (P > 0.05). Additionally, docking analysis showed that not only IL6R but also FCGR2A can be a ligand for TCZ. Conclusion This study provides valuable insights into the impact of genetic variations on the response rate of TCZ in COVID-19 patients. The GA genotype for FCGR2A rs1801274 was associated with poor treatment outcomes.
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spelling doaj-art-ebe08962aa874f63a31394ff93c3ea452025-08-20T02:17:39ZengBMCBMC Infectious Diseases1471-23342024-10-0124111110.1186/s12879-024-10073-0Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19Nastaran Injinari0Samira Asadollahi1Fateme Sefid2Maedeh Arshadi3Saeedeh Sadat Hosseini4Hamed Ghoshouni5Fatemeh Soltani6Nasim Namiranian7Mohammad Hasan Sheikhha8Fatemeh Aghaeimeybodi9Diabetes Research Center, Shahid Sadoughi University of Medical SciencesResearch Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical SciencesDepartment of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical SciencesDepartment of Epidemiology and Biostatistics, School of Public Health, Shahid Sadoughi University of Medical SciencesResearch Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical SciencesDiabetes Research Center, Shahid Sadoughi University of Medical SciencesDiabetes Research Center, Shahid Sadoughi University of Medical SciencesDiabetes Research Center, Shahid Sadoughi University of Medical SciencesAbortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical ScienceDepartment of Internal Medicine, Shahid Sadoughi University of Medical SciencesAbstract Background Although several genetic biomarkers have been reported in the tocilizumab (TCZ) response in rheumatoid arthritis, no studies have addressed the pharmacogenomics effect of TCZ in COVID-19. Methods In this prospective longitudinal study, 95 individuals with severe COVID-19 were selected between 2020–2022. The recovery process was measured at 24 h, 48 h, and 10 days before and after taking TCZ. All participants were genotyped using RFLP-PCR. Different genotypes of FCGR2A rs1801274 and IL-6R rs2228145 were compared in terms of the recovery process. Results 43.2% of patients were male and 56.8% were female with an average age of 58.20(± 16.214) years. The GA genotype for FCGR2A rs1801274 increased the risk of death (OR = 2.83, P = 0.038) and ventilation (OR = 2.71, P = 0.047) in TCZ-treated individuals. However, there was no risk of death and ventilation with IL-6R rs2228145 (P > 0.05). Additionally, docking analysis showed that not only IL6R but also FCGR2A can be a ligand for TCZ. Conclusion This study provides valuable insights into the impact of genetic variations on the response rate of TCZ in COVID-19 patients. The GA genotype for FCGR2A rs1801274 was associated with poor treatment outcomes.https://doi.org/10.1186/s12879-024-10073-0TocilizumabCOVID-19PolymorphismFCGR2AIL-6 receptor
spellingShingle Nastaran Injinari
Samira Asadollahi
Fateme Sefid
Maedeh Arshadi
Saeedeh Sadat Hosseini
Hamed Ghoshouni
Fatemeh Soltani
Nasim Namiranian
Mohammad Hasan Sheikhha
Fatemeh Aghaeimeybodi
Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
BMC Infectious Diseases
Tocilizumab
COVID-19
Polymorphism
FCGR2A
IL-6 receptor
title Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
title_full Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
title_fullStr Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
title_full_unstemmed Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
title_short Impact of FCGR2A rs1801274 and IL-6R rs2228145 polymorphisms on tocilizumab response in the Iranian population with severe COVID-19
title_sort impact of fcgr2a rs1801274 and il 6r rs2228145 polymorphisms on tocilizumab response in the iranian population with severe covid 19
topic Tocilizumab
COVID-19
Polymorphism
FCGR2A
IL-6 receptor
url https://doi.org/10.1186/s12879-024-10073-0
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