A novel lutein-stevioside complex inhibits DSS-induced colitis

Abstract Lutein is a natural strong antioxidant that has been reported to prevent inflammation and cancer in the body. However, its low solubility and bioavailability limit its efficacy. We previously prepared a highly water-soluble lutein-stevioside (LUT-STE) complex, which significantly improved t...

Full description

Saved in:
Bibliographic Details
Main Authors: Yangyang Dong, Mengdi Hao, Yayuan Xu, Lei Feng, Dajing Li, Yunhe Lian, Di Wu, Zhuqing Dai
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Food Production, Processing and Nutrition
Subjects:
Online Access:https://doi.org/10.1186/s43014-025-00310-7
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849704714465181696
author Yangyang Dong
Mengdi Hao
Yayuan Xu
Lei Feng
Dajing Li
Yunhe Lian
Di Wu
Zhuqing Dai
author_facet Yangyang Dong
Mengdi Hao
Yayuan Xu
Lei Feng
Dajing Li
Yunhe Lian
Di Wu
Zhuqing Dai
author_sort Yangyang Dong
collection DOAJ
description Abstract Lutein is a natural strong antioxidant that has been reported to prevent inflammation and cancer in the body. However, its low solubility and bioavailability limit its efficacy. We previously prepared a highly water-soluble lutein-stevioside (LUT-STE) complex, which significantly improved the bioavailability of lutein. This study aims to explore whether the complex can further improve its effectiveness in preventing colitis. In this study, a DSS-induced inflammatory bowel disease (IBD) model was used to explore whether the complex can further improve its effectiveness in preventing colitis. The results showed that both LUT-STE and LUT + STE showed a good inhibitory effect on colitis, which was better than their single treatment group. LUT and STE showed a synergistic effect in inhibiting colitis function. LUT and STE intervention could significantly improve the symptoms of DSS-induced colitis, including inhibiting weight loss, colon shortening, hematochezia, intestinal tissue damage, and secretion and expression of inflammatory factors. In addition, LUT and STE treatment increased mRNA expression levels of tight junction proteins ZO-1, Occludin, and Claudin-1, restored intestinal tissue integrity, and improved the expression of short-chain fatty acid receptor genes GRP41 and GRP43, which contributed to enhancing the production of medium-short chain fatty acids in mice. The STE encapsulates the LUT through adjustment. The relative abundance of Rikenellaceae, Prevotellaceae, Lachnospiraceae, Ruminococcaceae, Erysipelotrichaceae, Helicobacteraceae, and Bacteroidaceae regulate the ecological imbalance of intestinal microbiome and change the function of a microbial community. Therefore, LUT and STE can improve DSS-induced colitis by regulating intestinal flora structure and some vital intestinal microorganisms. LUT and STE may be promising substances for treating human IBD and provide the fundamental theoretical basis for developing nutritional prevention and intervention strategies. Graphical Abstract
format Article
id doaj-art-ebbd52e0532a4c8b8eacd0905d5ab766
institution DOAJ
issn 2661-8974
language English
publishDate 2025-05-01
publisher BMC
record_format Article
series Food Production, Processing and Nutrition
spelling doaj-art-ebbd52e0532a4c8b8eacd0905d5ab7662025-08-20T03:16:41ZengBMCFood Production, Processing and Nutrition2661-89742025-05-017111710.1186/s43014-025-00310-7A novel lutein-stevioside complex inhibits DSS-induced colitisYangyang Dong0Mengdi Hao1Yayuan Xu2Lei Feng3Dajing Li4Yunhe Lian5Di Wu6Zhuqing Dai7Institute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesChenguang Biotech Group Co., LtdChenguang Biotech Group Co., LtdInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural SciencesAbstract Lutein is a natural strong antioxidant that has been reported to prevent inflammation and cancer in the body. However, its low solubility and bioavailability limit its efficacy. We previously prepared a highly water-soluble lutein-stevioside (LUT-STE) complex, which significantly improved the bioavailability of lutein. This study aims to explore whether the complex can further improve its effectiveness in preventing colitis. In this study, a DSS-induced inflammatory bowel disease (IBD) model was used to explore whether the complex can further improve its effectiveness in preventing colitis. The results showed that both LUT-STE and LUT + STE showed a good inhibitory effect on colitis, which was better than their single treatment group. LUT and STE showed a synergistic effect in inhibiting colitis function. LUT and STE intervention could significantly improve the symptoms of DSS-induced colitis, including inhibiting weight loss, colon shortening, hematochezia, intestinal tissue damage, and secretion and expression of inflammatory factors. In addition, LUT and STE treatment increased mRNA expression levels of tight junction proteins ZO-1, Occludin, and Claudin-1, restored intestinal tissue integrity, and improved the expression of short-chain fatty acid receptor genes GRP41 and GRP43, which contributed to enhancing the production of medium-short chain fatty acids in mice. The STE encapsulates the LUT through adjustment. The relative abundance of Rikenellaceae, Prevotellaceae, Lachnospiraceae, Ruminococcaceae, Erysipelotrichaceae, Helicobacteraceae, and Bacteroidaceae regulate the ecological imbalance of intestinal microbiome and change the function of a microbial community. Therefore, LUT and STE can improve DSS-induced colitis by regulating intestinal flora structure and some vital intestinal microorganisms. LUT and STE may be promising substances for treating human IBD and provide the fundamental theoretical basis for developing nutritional prevention and intervention strategies. Graphical Abstracthttps://doi.org/10.1186/s43014-025-00310-7LuteinSteviosideGut barrierGut microbiomeInflammatory bowel disease
spellingShingle Yangyang Dong
Mengdi Hao
Yayuan Xu
Lei Feng
Dajing Li
Yunhe Lian
Di Wu
Zhuqing Dai
A novel lutein-stevioside complex inhibits DSS-induced colitis
Food Production, Processing and Nutrition
Lutein
Stevioside
Gut barrier
Gut microbiome
Inflammatory bowel disease
title A novel lutein-stevioside complex inhibits DSS-induced colitis
title_full A novel lutein-stevioside complex inhibits DSS-induced colitis
title_fullStr A novel lutein-stevioside complex inhibits DSS-induced colitis
title_full_unstemmed A novel lutein-stevioside complex inhibits DSS-induced colitis
title_short A novel lutein-stevioside complex inhibits DSS-induced colitis
title_sort novel lutein stevioside complex inhibits dss induced colitis
topic Lutein
Stevioside
Gut barrier
Gut microbiome
Inflammatory bowel disease
url https://doi.org/10.1186/s43014-025-00310-7
work_keys_str_mv AT yangyangdong anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT mengdihao anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT yayuanxu anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT leifeng anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT dajingli anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT yunhelian anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT diwu anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT zhuqingdai anovelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT yangyangdong novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT mengdihao novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT yayuanxu novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT leifeng novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT dajingli novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT yunhelian novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT diwu novelluteinsteviosidecomplexinhibitsdssinducedcolitis
AT zhuqingdai novelluteinsteviosidecomplexinhibitsdssinducedcolitis