Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum
Chloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking a...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7930102 |
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| _version_ | 1832558070701490176 |
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| author | Shanwen Zhao Chanling Wei Zhian Sun Huachun Liu Yanqiang Zhou Xiaoxiao Wang Jianmin Li Bolin Gong |
| author_facet | Shanwen Zhao Chanling Wei Zhian Sun Huachun Liu Yanqiang Zhou Xiaoxiao Wang Jianmin Li Bolin Gong |
| author_sort | Shanwen Zhao |
| collection | DOAJ |
| description | Chloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. The CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. The adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. The CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with high-performance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7–1.5%. This material has broad application prospects in enrichment and separation. |
| format | Article |
| id | doaj-art-ebae0765fb1c46119e871d92f7b88709 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-ebae0765fb1c46119e871d92f7b887092025-02-03T01:33:29ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/79301027930102Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine SerumShanwen Zhao0Chanling Wei1Zhian Sun2Huachun Liu3Yanqiang Zhou4Xiaoxiao Wang5Jianmin Li6Bolin Gong7College of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaCollege of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaChloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. The CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. The adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. The CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with high-performance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7–1.5%. This material has broad application prospects in enrichment and separation.http://dx.doi.org/10.1155/2019/7930102 |
| spellingShingle | Shanwen Zhao Chanling Wei Zhian Sun Huachun Liu Yanqiang Zhou Xiaoxiao Wang Jianmin Li Bolin Gong Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum Journal of Analytical Methods in Chemistry |
| title | Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum |
| title_full | Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum |
| title_fullStr | Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum |
| title_full_unstemmed | Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum |
| title_short | Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum |
| title_sort | preparation of restricted access media molecularly imprinted polymers for the detection of chloramphenicol in bovine serum |
| url | http://dx.doi.org/10.1155/2019/7930102 |
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