Hair growth-promoting effects of Stellera chamaejasme L. extract on C57BL/6 mice via induction of anagen phase genes

Objective: To evaluate the effects of Stellera chamaejasme L. (S. chamaejasme, Rui Xiang Lang Du) extract on hair growth in a mouse model. Methods: The extract was prepared using 95% ethanol and topically applied as a 1% or 3% solution to the dorsal skin of shaved mice for 16 consecutive days. A con...

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Main Authors: Jin-Woo Kim, Banzragch Dorjsembe, Chu Won Nho, Pahn-Shick Chang, Jin-Chul Kim
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Journal of Traditional Chinese Medical Sciences
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095754825000511
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Summary:Objective: To evaluate the effects of Stellera chamaejasme L. (S. chamaejasme, Rui Xiang Lang Du) extract on hair growth in a mouse model. Methods: The extract was prepared using 95% ethanol and topically applied as a 1% or 3% solution to the dorsal skin of shaved mice for 16 consecutive days. A control mouse group received an equal volume of vehicle for the same period. After 16 days, the dorsal skin was histologically examined through hematoxylin-eosin staining. Further, quantitative real time-polymerase chain reaction was performed on skin tissue lysates to evaluate the expression levels of mRNAs encoding proteins involved in hair growth, including WNT10A, noggin (NOG), transforming growth factor-β receptor 1 (TBR1), epidermal growth factor (EGF), versican, fibroblast growth factor 10 (FGF10), lymphoid enhancer-binding factor 1 (LEF1), and transforming growth factor-β (TGF-β). Results: Compared with vehicle, S. chamaejasme extract dose-dependently enhanced hair growth. Histological analysis revealed that S. chamaejasme extract increased the number and diameter of hair follicles in subcutaneous tissue, as well as dermal layer thickness, which are indicative of anagen phase induction. Additionally, S. chamaejasme extract upregulated the mRNA expression levels of WNT10A, NOG, TBR1, EFG, FGF10, LEF1, and TGF-β. Conclusion: The results suggest that S. chamaejasme extract could be a potential treatment for promoting hair growth.
ISSN:2095-7548