Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models
Background/Objectives: The ongoing COVID-19 pandemic has underscored the need for alternative prophylactic measures, particularly for populations for whom vaccines may not be effective or accessible. This study aims to evaluate the efficacy of intranasally administered IgY antibodies derived from he...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2076-393X/12/12/1422 |
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| author | Mónika Madai Dániel Hanna Roland Hetényi Fanni Földes Zsófia Lanszki Brigitta Zana Balázs Somogyi Henrietta Papp Anett Kuczmog Orsolya Faragó-Sipos Csaba Nemes Vilmos Palya Dávid Géza Horváth Gyula Balka Krisztián Bányai Xinkai Jia Péter Balogh Pál Bajnóczi |
| author_facet | Mónika Madai Dániel Hanna Roland Hetényi Fanni Földes Zsófia Lanszki Brigitta Zana Balázs Somogyi Henrietta Papp Anett Kuczmog Orsolya Faragó-Sipos Csaba Nemes Vilmos Palya Dávid Géza Horváth Gyula Balka Krisztián Bányai Xinkai Jia Péter Balogh Pál Bajnóczi |
| author_sort | Mónika Madai |
| collection | DOAJ |
| description | Background/Objectives: The ongoing COVID-19 pandemic has underscored the need for alternative prophylactic measures, particularly for populations for whom vaccines may not be effective or accessible. This study aims to evaluate the efficacy of intranasally administered IgY antibodies derived from hen egg yolks as a protective agent against SARS-CoV-2 infection in Syrian golden hamsters, a well-established animal model for COVID-19. Methods: Hens were immunized with the spike protein of SARS-CoV-2 to generate IgY antibodies. These antibodies were extracted from the egg yolks, purified, and their neutralizing activity was tested in vitro. Syrian golden hamsters were then treated with the IgY antibodies before being challenged with SARS-CoV-2. Viral loads were quantified using droplet digital PCR (ddPCR), and lung pathology was assessed through histopathological analysis. Results: The in vitro assays showed that IgY effectively neutralized SARS-CoV-2. In the in vivo hamster model, IgY treatment led to a significant reduction in viral loads and a marked decrease in lung consolidation and inflammation compared to the positive control group. Histopathological findings further supported the protective role of IgY in reducing lung damage caused by SARS-CoV-2. Conclusions: The results demonstrate that IgY antibodies exhibit strong antiviral activity and can significantly reduce SARS-CoV-2 viral loads and associated lung pathology in hamsters. These findings suggest that IgY could be a viable prophylactic option for preventing SARS-CoV-2 infection, particularly for individuals who cannot receive or respond to vaccines. Further studies are warranted to optimize dosage and explore the long-term efficacy of IgY antibodies. |
| format | Article |
| id | doaj-art-eb9f1a59da7d4e07853e3b12a77bd4a4 |
| institution | OA Journals |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-eb9f1a59da7d4e07853e3b12a77bd4a42025-08-20T02:01:14ZengMDPI AGVaccines2076-393X2024-12-011212142210.3390/vaccines12121422Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster ModelsMónika Madai0Dániel Hanna1Roland Hetényi2Fanni Földes3Zsófia Lanszki4Brigitta Zana5Balázs Somogyi6Henrietta Papp7Anett Kuczmog8Orsolya Faragó-Sipos9Csaba Nemes10Vilmos Palya11Dávid Géza Horváth12Gyula Balka13Krisztián Bányai14Xinkai Jia15Péter Balogh16Pál Bajnóczi17National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryProphyl Kft., 7700 Mohács, HungaryProphyl Kft., 7700 Mohács, HungaryProphyl Kft., 7700 Mohács, HungaryDepartment of Pathology, University of Veterinary Medicine, 1078 Budapest, HungaryDepartment of Pathology, University of Veterinary Medicine, 1078 Budapest, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, 7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, HungaryProphyl Kft., 7700 Mohács, HungaryBackground/Objectives: The ongoing COVID-19 pandemic has underscored the need for alternative prophylactic measures, particularly for populations for whom vaccines may not be effective or accessible. This study aims to evaluate the efficacy of intranasally administered IgY antibodies derived from hen egg yolks as a protective agent against SARS-CoV-2 infection in Syrian golden hamsters, a well-established animal model for COVID-19. Methods: Hens were immunized with the spike protein of SARS-CoV-2 to generate IgY antibodies. These antibodies were extracted from the egg yolks, purified, and their neutralizing activity was tested in vitro. Syrian golden hamsters were then treated with the IgY antibodies before being challenged with SARS-CoV-2. Viral loads were quantified using droplet digital PCR (ddPCR), and lung pathology was assessed through histopathological analysis. Results: The in vitro assays showed that IgY effectively neutralized SARS-CoV-2. In the in vivo hamster model, IgY treatment led to a significant reduction in viral loads and a marked decrease in lung consolidation and inflammation compared to the positive control group. Histopathological findings further supported the protective role of IgY in reducing lung damage caused by SARS-CoV-2. Conclusions: The results demonstrate that IgY antibodies exhibit strong antiviral activity and can significantly reduce SARS-CoV-2 viral loads and associated lung pathology in hamsters. These findings suggest that IgY could be a viable prophylactic option for preventing SARS-CoV-2 infection, particularly for individuals who cannot receive or respond to vaccines. Further studies are warranted to optimize dosage and explore the long-term efficacy of IgY antibodies.https://www.mdpi.com/2076-393X/12/12/1422IgY antibodiesSARS-CoV-2COVID-19 prophylaxisSyrian golden hamsterviral neutralization |
| spellingShingle | Mónika Madai Dániel Hanna Roland Hetényi Fanni Földes Zsófia Lanszki Brigitta Zana Balázs Somogyi Henrietta Papp Anett Kuczmog Orsolya Faragó-Sipos Csaba Nemes Vilmos Palya Dávid Géza Horváth Gyula Balka Krisztián Bányai Xinkai Jia Péter Balogh Pál Bajnóczi Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models Vaccines IgY antibodies SARS-CoV-2 COVID-19 prophylaxis Syrian golden hamster viral neutralization |
| title | Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models |
| title_full | Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models |
| title_fullStr | Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models |
| title_full_unstemmed | Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models |
| title_short | Evaluating the Protective Role of Intranasally Administered Avian-Derived IgY Against SARS-CoV-2 in Syrian Hamster Models |
| title_sort | evaluating the protective role of intranasally administered avian derived igy against sars cov 2 in syrian hamster models |
| topic | IgY antibodies SARS-CoV-2 COVID-19 prophylaxis Syrian golden hamster viral neutralization |
| url | https://www.mdpi.com/2076-393X/12/12/1422 |
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