Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition
The purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, f...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8837526 |
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| author | Min Wei Yangling Tuo Ye Zhang Qi Deng Cuiying Shi Xuexian Chen Xu Zhang |
| author_facet | Min Wei Yangling Tuo Ye Zhang Qi Deng Cuiying Shi Xuexian Chen Xu Zhang |
| author_sort | Min Wei |
| collection | DOAJ |
| description | The purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, filter out the key compounds, and evaluate the quality of 26 batches of tender leaves and flower spikes of Lithocarpus polystachyus Rehd. (LP) from ten areas in China. The result showed that the HPLC-DAD method had excellent performance for accurate quantification analysis. S3 (tender leaf from Lushan, Sichuan) had the highest contents for six measured chemicals with trilobatin content of up to 27.82% in dry weight. S22 (flower spike from Liangping, Chongqing) had the highest content of phloridzin (up to 7.28%). All samples were divided into three types based on spatial distribution using principal component analysis. The result showed that the tender leaves and flower spikes from the same areas had many similar properties, and there were significant differences between the samples from different regions. Furthermore, phloridzin and trilobatin were identified as chemical markers for quality evaluation of two parts with different tender leaves and flower spikes of LP from geographical areas by orthogonal partial least squares discrimination analysis. These results will be helpful to establish an effective and comprehensive evaluation system of the development and utilization of LP resources. |
| format | Article |
| id | doaj-art-eb872f84edd04a0f97d8a283ea765e46 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-eb872f84edd04a0f97d8a283ea765e462025-02-03T01:20:21ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88375268837526Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern RecognitionMin Wei0Yangling Tuo1Ye Zhang2Qi Deng3Cuiying Shi4Xuexian Chen5Xu Zhang6College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaPeople’s Hospital of Luxian County, Luzhou 646100, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaChengdu Traditional Chinese Medicine Health Care Technology Co. Ltd., Chengdu 610000, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaThe purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, filter out the key compounds, and evaluate the quality of 26 batches of tender leaves and flower spikes of Lithocarpus polystachyus Rehd. (LP) from ten areas in China. The result showed that the HPLC-DAD method had excellent performance for accurate quantification analysis. S3 (tender leaf from Lushan, Sichuan) had the highest contents for six measured chemicals with trilobatin content of up to 27.82% in dry weight. S22 (flower spike from Liangping, Chongqing) had the highest content of phloridzin (up to 7.28%). All samples were divided into three types based on spatial distribution using principal component analysis. The result showed that the tender leaves and flower spikes from the same areas had many similar properties, and there were significant differences between the samples from different regions. Furthermore, phloridzin and trilobatin were identified as chemical markers for quality evaluation of two parts with different tender leaves and flower spikes of LP from geographical areas by orthogonal partial least squares discrimination analysis. These results will be helpful to establish an effective and comprehensive evaluation system of the development and utilization of LP resources.http://dx.doi.org/10.1155/2020/8837526 |
| spellingShingle | Min Wei Yangling Tuo Ye Zhang Qi Deng Cuiying Shi Xuexian Chen Xu Zhang Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition Journal of Analytical Methods in Chemistry |
| title | Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition |
| title_full | Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition |
| title_fullStr | Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition |
| title_full_unstemmed | Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition |
| title_short | Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition |
| title_sort | evaluation of two parts of lithocarpus polystachyus rehd from different chinese areas by multicomponent content determination and pattern recognition |
| url | http://dx.doi.org/10.1155/2020/8837526 |
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