The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology
Lignocellulosic biomass derived from food waste, microalgae, and agroresidues is key in the circular bioeconomy, promoting eco-friendly bioethanol production while addressing waste management challenges. This paper aimed to comprehensively review the role of amylases secreted from different strains...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Fuel Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266605202500010X |
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| author | Eshet Lakew Tesfaye Prince Kumar Pannaga Pavan Jutur Anteneh Tesfaye Tefera Tamene Milkessa Jiru Naseem A. Gaur |
| author_facet | Eshet Lakew Tesfaye Prince Kumar Pannaga Pavan Jutur Anteneh Tesfaye Tefera Tamene Milkessa Jiru Naseem A. Gaur |
| author_sort | Eshet Lakew Tesfaye |
| collection | DOAJ |
| description | Lignocellulosic biomass derived from food waste, microalgae, and agroresidues is key in the circular bioeconomy, promoting eco-friendly bioethanol production while addressing waste management challenges. This paper aimed to comprehensively review the role of amylases secreted from different strains in bioethanol production. The sources and optimizing factors critical for effective starch breakdown are discussed. Amylase, derived from various organisms, constitutes an essential enzyme and facilitates starch hydrolysis into fermentable sugars like glucose, a key step in bioethanol production. However, its activity depends on factors such as pH, temperature, incubation time, and the substrates used, necessitating optimization for efficient starch degradation. Moreover, the review also explores strategies to engineer amylase-producing strains with enhanced amylase production capabilities using an advanced clustered regularly interspaced short palindromic repeat (CRISPR/Cas9)-based gene editing technology. Further research is crucial to identify novel amylase-producing strains from different environments, including from extremophiles, characterize their enzymes, and leverage tools like CRISPR/Cas9-mediated genome editing in microorganisms, and leverage these advancements to ensure sustainable bioethanol production. |
| format | Article |
| id | doaj-art-eb73a0f22b504d20a6e51eb7b00e7b4a |
| institution | DOAJ |
| issn | 2666-0520 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fuel Communications |
| spelling | doaj-art-eb73a0f22b504d20a6e51eb7b00e7b4a2025-08-20T03:10:20ZengElsevierFuel Communications2666-05202025-06-012310014210.1016/j.jfueco.2025.100142The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 TechnologyEshet Lakew Tesfaye0Prince Kumar1Pannaga Pavan Jutur2Anteneh Tesfaye Tefera3Tamene Milkessa Jiru4Naseem A. Gaur5Department of Biotechnology, College of Natural and Computational Sciences, Hawassa University, Hawassa, Ethiopia; Corresponding author.Electrical Engineering Department, NIT Silchar, Silchar, Assam, IndiaOmics of Algae Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaInstitute of Biotechnology, Addis Ababa University, Addis Ababa, EthiopiaDepartment of Environmental and Industrial Biotechnology, Institute of Biotechnology, University of Gondar, P.O. Box: 196, Gondar, EthiopiaYeast Biofuel Group, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, IndiaLignocellulosic biomass derived from food waste, microalgae, and agroresidues is key in the circular bioeconomy, promoting eco-friendly bioethanol production while addressing waste management challenges. This paper aimed to comprehensively review the role of amylases secreted from different strains in bioethanol production. The sources and optimizing factors critical for effective starch breakdown are discussed. Amylase, derived from various organisms, constitutes an essential enzyme and facilitates starch hydrolysis into fermentable sugars like glucose, a key step in bioethanol production. However, its activity depends on factors such as pH, temperature, incubation time, and the substrates used, necessitating optimization for efficient starch degradation. Moreover, the review also explores strategies to engineer amylase-producing strains with enhanced amylase production capabilities using an advanced clustered regularly interspaced short palindromic repeat (CRISPR/Cas9)-based gene editing technology. Further research is crucial to identify novel amylase-producing strains from different environments, including from extremophiles, characterize their enzymes, and leverage tools like CRISPR/Cas9-mediated genome editing in microorganisms, and leverage these advancements to ensure sustainable bioethanol production.http://www.sciencedirect.com/science/article/pii/S266605202500010XCRISPRECGRASgRNAHDRNGS |
| spellingShingle | Eshet Lakew Tesfaye Prince Kumar Pannaga Pavan Jutur Anteneh Tesfaye Tefera Tamene Milkessa Jiru Naseem A. Gaur The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology Fuel Communications CRISPR EC GRAS gRNA HDR NGS |
| title | The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology |
| title_full | The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology |
| title_fullStr | The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology |
| title_full_unstemmed | The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology |
| title_short | The role of amylase in bioethanol production: advances in amylase-producing strains using CRISPR/Cas9 Technology |
| title_sort | role of amylase in bioethanol production advances in amylase producing strains using crispr cas9 technology |
| topic | CRISPR EC GRAS gRNA HDR NGS |
| url | http://www.sciencedirect.com/science/article/pii/S266605202500010X |
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