Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle
Maternal nutrition’s impact on liver transcriptome in beef cattle offspring is still underexplored. We investigated the long-term effects of maternal nutrition strategies on the liver transcriptome of pre-slaughter Nelore bulls. Pregnant cows were divided into three groups, each receiving different...
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MDPI AG
2025-04-01
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| author | Guilherme Henrique Gebim Polizel Maria Elis Perissin dos Santos Aline Silva Mello Cesar Wellison J. S. Diniz German D. Ramírez-Zamudio Paulo Fantinato-Neto Arícia Christofaro Fernandes Barbara Carolina Teixeira Prati Édison Furlan Gabriela do Vale Pombo Miguel Henrique de Almeida Santana |
| author_facet | Guilherme Henrique Gebim Polizel Maria Elis Perissin dos Santos Aline Silva Mello Cesar Wellison J. S. Diniz German D. Ramírez-Zamudio Paulo Fantinato-Neto Arícia Christofaro Fernandes Barbara Carolina Teixeira Prati Édison Furlan Gabriela do Vale Pombo Miguel Henrique de Almeida Santana |
| author_sort | Guilherme Henrique Gebim Polizel |
| collection | DOAJ |
| description | Maternal nutrition’s impact on liver transcriptome in beef cattle offspring is still underexplored. We investigated the long-term effects of maternal nutrition strategies on the liver transcriptome of pre-slaughter Nelore bulls. Pregnant cows were divided into three groups, each receiving different nutritional regimens: NP (control, only mineral supplementation), PP (late gestation protein–energy supplementation), and FP (protein–energy supplementation throughout pregnancy). Liver samples were collected from male offspring aged 22.5 ± 1 months and analyzed using RNA-Seq (n = 5 per treatment). Principal component analysis (PCA) and differential gene expression analysis were carried out in an R statistical environment. Genes were considered significant when FDR < 0.05. The over-representation analysis (ORA) was performed using the clusterProfiler package from R. Metabolic pathways were considered significant when the Q-value < 0.1. The PCA showed overlapping clusters among the groups. We identified 16 differentially expressed genes (DEGs) associated with PP × NP contrast, four with FP × NP, and two with FP × PP. The ORA revealed two significant pathways (thiamine and butanoate metabolism). The identified genes and pathways were associated with vitamins, energy, oxidative metabolism, and immune function. This study emphasizes the more significant long-term effects of the PP treatment on the offspring’s liver transcriptome compared to the FP treatment. |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Veterinary Sciences |
| spelling | doaj-art-eb5efa424e3d42e099668a9c01faa9672025-08-20T01:56:45ZengMDPI AGVeterinary Sciences2306-73812025-04-0112540610.3390/vetsci12050406Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef CattleGuilherme Henrique Gebim Polizel0Maria Elis Perissin dos Santos1Aline Silva Mello Cesar2Wellison J. S. Diniz3German D. Ramírez-Zamudio4Paulo Fantinato-Neto5Arícia Christofaro Fernandes6Barbara Carolina Teixeira Prati7Édison Furlan8Gabriela do Vale Pombo9Miguel Henrique de Almeida Santana10Department of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Food Science and Technology, Luiz de Queiroz College of Agriculture, University of São Paulo, Av. Pádua Dias 11, Piracicaba 13418-900, SP, BrazilDepartment of Animal Sciences, College of Agriculture, Auburn University, Auburn, AL 36849, USADepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilÁPIS Serviços Veterinários LTDA, R. Marques de Tamandaré, 130, Jardim Elite, Pirassununga 13636-350, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilMaternal nutrition’s impact on liver transcriptome in beef cattle offspring is still underexplored. We investigated the long-term effects of maternal nutrition strategies on the liver transcriptome of pre-slaughter Nelore bulls. Pregnant cows were divided into three groups, each receiving different nutritional regimens: NP (control, only mineral supplementation), PP (late gestation protein–energy supplementation), and FP (protein–energy supplementation throughout pregnancy). Liver samples were collected from male offspring aged 22.5 ± 1 months and analyzed using RNA-Seq (n = 5 per treatment). Principal component analysis (PCA) and differential gene expression analysis were carried out in an R statistical environment. Genes were considered significant when FDR < 0.05. The over-representation analysis (ORA) was performed using the clusterProfiler package from R. Metabolic pathways were considered significant when the Q-value < 0.1. The PCA showed overlapping clusters among the groups. We identified 16 differentially expressed genes (DEGs) associated with PP × NP contrast, four with FP × NP, and two with FP × PP. The ORA revealed two significant pathways (thiamine and butanoate metabolism). The identified genes and pathways were associated with vitamins, energy, oxidative metabolism, and immune function. This study emphasizes the more significant long-term effects of the PP treatment on the offspring’s liver transcriptome compared to the FP treatment.https://www.mdpi.com/2306-7381/12/5/406energy metabolismfetal programminggene expressionimmune systemthiamine metabolism |
| spellingShingle | Guilherme Henrique Gebim Polizel Maria Elis Perissin dos Santos Aline Silva Mello Cesar Wellison J. S. Diniz German D. Ramírez-Zamudio Paulo Fantinato-Neto Arícia Christofaro Fernandes Barbara Carolina Teixeira Prati Édison Furlan Gabriela do Vale Pombo Miguel Henrique de Almeida Santana Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle Veterinary Sciences energy metabolism fetal programming gene expression immune system thiamine metabolism |
| title | Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle |
| title_full | Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle |
| title_fullStr | Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle |
| title_full_unstemmed | Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle |
| title_short | Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle |
| title_sort | late gestation maternal nutrition has a stronger impact on offspring liver transcriptome than full gestation supplementation in beef cattle |
| topic | energy metabolism fetal programming gene expression immune system thiamine metabolism |
| url | https://www.mdpi.com/2306-7381/12/5/406 |
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