Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats

Endotoxemia induces a series of inflammatory responses that may result in lung injury. However, heat shock protein72 (HSP72) has the potential to protect the lungs from damage. The objective of this study was to determine whether prior exercise conditioning could increase the expression of HSP72 in...

Full description

Saved in:
Bibliographic Details
Main Authors: Ching-Hsia Hung, Jann-Inn Tzeng, Che-Ning Chang, Yu-Wen Chen, Chia-Ying Cho, Jhi-Joung Wang
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/2013/527090
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850163128199806976
author Ching-Hsia Hung
Jann-Inn Tzeng
Che-Ning Chang
Yu-Wen Chen
Chia-Ying Cho
Jhi-Joung Wang
author_facet Ching-Hsia Hung
Jann-Inn Tzeng
Che-Ning Chang
Yu-Wen Chen
Chia-Ying Cho
Jhi-Joung Wang
author_sort Ching-Hsia Hung
collection DOAJ
description Endotoxemia induces a series of inflammatory responses that may result in lung injury. However, heat shock protein72 (HSP72) has the potential to protect the lungs from damage. The objective of this study was to determine whether prior exercise conditioning could increase the expression of HSP72 in the lungs and attenuate lung damage in diabetic rats receiving lipopolysaccharide (LPS). Streptozotocin was used to induce diabetes in adult male Wistar rats. Rats were randomly assigned to sedentary or exercise groups. Rats in the exercise condition ran on a treadmill 5 days/week, 30–60 min/day, with an intensity of 1.0 mile/hour over a 3-week period. Rats received an intravenous infusion of LPS after 24 hrs from the last training session. Elevated lavage tumor necrosis factor-alpha (TNF-α) level in response to LPS was more marked in diabetic rats. HSP72 expression in lungs was significantly increased after exercise conditioning, but less pronounced in diabetic rats. After administration of LPS, exercised rats displayed higher survival rate as well as decreased lavage TNF-α level and lung edema in comparison to sedentary rats. Our findings suggest that exercise conditioning could attenuate the occurrence of inflammatory responses and lung damage, thereby reducing mortality rate in diabetic rats during endotoxemia.
format Article
id doaj-art-eb478edf87254cf490f3addcd778958a
institution OA Journals
issn 2314-6745
2314-6753
language English
publishDate 2013-01-01
publisher Wiley
record_format Article
series Journal of Diabetes Research
spelling doaj-art-eb478edf87254cf490f3addcd778958a2025-08-20T02:22:21ZengWileyJournal of Diabetes Research2314-67452314-67532013-01-01201310.1155/2013/527090527090Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic RatsChing-Hsia Hung0Jann-Inn Tzeng1Che-Ning Chang2Yu-Wen Chen3Chia-Ying Cho4Jhi-Joung Wang5Department of Physical Therapy, National Cheng Kung University, Tainan 701, TaiwanDepartment of Food Sciences and Technology, Chia Nan University of Pharmacy and Sciences, Jen-Te, Tainan 717, TaiwanDepartment of Physical Therapy, National Cheng Kung University, Tainan 701, TaiwanDepartment of Physical Therapy, China Medical University, Taichung 404, TaiwanDepartment of Physical Therapy, National Cheng Kung University, Tainan 701, TaiwanDepartment of Medical Research, Chi-Mei Medical Centre, Tainan 710, TaiwanEndotoxemia induces a series of inflammatory responses that may result in lung injury. However, heat shock protein72 (HSP72) has the potential to protect the lungs from damage. The objective of this study was to determine whether prior exercise conditioning could increase the expression of HSP72 in the lungs and attenuate lung damage in diabetic rats receiving lipopolysaccharide (LPS). Streptozotocin was used to induce diabetes in adult male Wistar rats. Rats were randomly assigned to sedentary or exercise groups. Rats in the exercise condition ran on a treadmill 5 days/week, 30–60 min/day, with an intensity of 1.0 mile/hour over a 3-week period. Rats received an intravenous infusion of LPS after 24 hrs from the last training session. Elevated lavage tumor necrosis factor-alpha (TNF-α) level in response to LPS was more marked in diabetic rats. HSP72 expression in lungs was significantly increased after exercise conditioning, but less pronounced in diabetic rats. After administration of LPS, exercised rats displayed higher survival rate as well as decreased lavage TNF-α level and lung edema in comparison to sedentary rats. Our findings suggest that exercise conditioning could attenuate the occurrence of inflammatory responses and lung damage, thereby reducing mortality rate in diabetic rats during endotoxemia.http://dx.doi.org/10.1155/2013/527090
spellingShingle Ching-Hsia Hung
Jann-Inn Tzeng
Che-Ning Chang
Yu-Wen Chen
Chia-Ying Cho
Jhi-Joung Wang
Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
Journal of Diabetes Research
title Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
title_full Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
title_fullStr Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
title_full_unstemmed Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
title_short Treadmill Exercise Preconditioning Attenuates Lung Damage Caused by Systemic Endotoxemia in Type 1 Diabetic Rats
title_sort treadmill exercise preconditioning attenuates lung damage caused by systemic endotoxemia in type 1 diabetic rats
url http://dx.doi.org/10.1155/2013/527090
work_keys_str_mv AT chinghsiahung treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats
AT janninntzeng treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats
AT cheningchang treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats
AT yuwenchen treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats
AT chiayingcho treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats
AT jhijoungwang treadmillexercisepreconditioningattenuateslungdamagecausedbysystemicendotoxemiaintype1diabeticrats