Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients
Chronic rhinosinusitis (CRS) is a persistent inflammatory condition of the sinus mucosa characterized by significant tissue remodeling. This study aimed to evaluate the gene expression of extracellular matrix (ECM) proteins, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinas...
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MDPI AG
2025-04-01
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| author | Zygimantas Vaitkus Astra Vitkauskiene Liutauras Labanauskas Justinas Vaitkus Povilas Lozovskis Saulius Vaitkus Ieva Janulaityte |
| author_facet | Zygimantas Vaitkus Astra Vitkauskiene Liutauras Labanauskas Justinas Vaitkus Povilas Lozovskis Saulius Vaitkus Ieva Janulaityte |
| author_sort | Zygimantas Vaitkus |
| collection | DOAJ |
| description | Chronic rhinosinusitis (CRS) is a persistent inflammatory condition of the sinus mucosa characterized by significant tissue remodeling. This study aimed to evaluate the gene expression of extracellular matrix (ECM) proteins, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) in post-operative tissues of CRS patients. A total of 30 patients diagnosed with CRS, divided into CRSwNP (with nasal polyps) and CRSsNP (without nasal polyps) groups, were compared with a control group of 10 individuals undergoing nasal surgeries for non-CRS conditions. Gene expression analysis was conducted using quantitative real-time PCR, and plasma cytokine levels were measured via ELISA. Results indicated significantly higher expression of <i>collagen I</i>, <i>collagen III</i>, <i>fibronectin</i>, <i>vimentin</i>, <i>periostin</i>, and <i>tenascin C</i> in CRS tissues, especially in CRSsNP patients. Conversely, <i>elastin</i> expression was markedly lower. <i>MMP-2</i>, <i>MMP-9</i>, <i>TIMP-1</i>, and <i>TIMP-2</i> expression was significantly altered, with CRSsNP showing lower levels compared to CRSwNP and controls. <i>TGF-β1</i> expression was elevated in both CRS groups, particularly in CRSsNP, highlighting its role in fibrosis and ECM remodeling. Additionally, increased plasma concentrations of TSLP and TGF-<i>β</i>1 suggest epithelial activation and immune dysregulation in CRS. These findings underscore distinct remodeling profiles in CRS endotypes, emphasizing the need for targeted therapeutic strategies based on molecular phenotyping. Understanding ECM dysregulation and inflammatory pathways in CRS may lead to improved, individualized treatment approaches. |
| format | Article |
| id | doaj-art-eb3bd6d18e544605aaf5fb32ee42bfb6 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-eb3bd6d18e544605aaf5fb32ee42bfb62025-08-20T03:52:58ZengMDPI AGCells2073-44092025-04-0114965410.3390/cells14090654Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis PatientsZygimantas Vaitkus0Astra Vitkauskiene1Liutauras Labanauskas2Justinas Vaitkus3Povilas Lozovskis4Saulius Vaitkus5Ieva Janulaityte6Department of Ear, Nose and Throat, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Laboratory Medicine, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Pediatrics, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Ear, Nose and Throat, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Laboratory Medicine, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Ear, Nose and Throat, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Laboratory Medicine, Faculty of Medicine, Academy of Medicine at Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaChronic rhinosinusitis (CRS) is a persistent inflammatory condition of the sinus mucosa characterized by significant tissue remodeling. This study aimed to evaluate the gene expression of extracellular matrix (ECM) proteins, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) in post-operative tissues of CRS patients. A total of 30 patients diagnosed with CRS, divided into CRSwNP (with nasal polyps) and CRSsNP (without nasal polyps) groups, were compared with a control group of 10 individuals undergoing nasal surgeries for non-CRS conditions. Gene expression analysis was conducted using quantitative real-time PCR, and plasma cytokine levels were measured via ELISA. Results indicated significantly higher expression of <i>collagen I</i>, <i>collagen III</i>, <i>fibronectin</i>, <i>vimentin</i>, <i>periostin</i>, and <i>tenascin C</i> in CRS tissues, especially in CRSsNP patients. Conversely, <i>elastin</i> expression was markedly lower. <i>MMP-2</i>, <i>MMP-9</i>, <i>TIMP-1</i>, and <i>TIMP-2</i> expression was significantly altered, with CRSsNP showing lower levels compared to CRSwNP and controls. <i>TGF-β1</i> expression was elevated in both CRS groups, particularly in CRSsNP, highlighting its role in fibrosis and ECM remodeling. Additionally, increased plasma concentrations of TSLP and TGF-<i>β</i>1 suggest epithelial activation and immune dysregulation in CRS. These findings underscore distinct remodeling profiles in CRS endotypes, emphasizing the need for targeted therapeutic strategies based on molecular phenotyping. Understanding ECM dysregulation and inflammatory pathways in CRS may lead to improved, individualized treatment approaches.https://www.mdpi.com/2073-4409/14/9/654extracellular matrixcollagenfibronectinMMPTIMPchronic rhinosinusitis |
| spellingShingle | Zygimantas Vaitkus Astra Vitkauskiene Liutauras Labanauskas Justinas Vaitkus Povilas Lozovskis Saulius Vaitkus Ieva Janulaityte Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients Cells extracellular matrix collagen fibronectin MMP TIMP chronic rhinosinusitis |
| title | Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients |
| title_full | Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients |
| title_fullStr | Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients |
| title_full_unstemmed | Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients |
| title_short | Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients |
| title_sort | gene expression of extracellular matrix proteins mmps and timps in post operative tissues of chronic rhinosinusitis patients |
| topic | extracellular matrix collagen fibronectin MMP TIMP chronic rhinosinusitis |
| url | https://www.mdpi.com/2073-4409/14/9/654 |
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