542 The association between cell-free DNA and lung transplant survival
Objectives/Goals: Lung transplant is a life-saving surgery for patients with advanced lung diseases yet long-term survival remains poor. The clinical features and lung injury patterns of lung transplant recipients who die early versus those who survive longer term remain undefined. Here, we use cell...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2025-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866124011142/type/journal_article |
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| author | Rohan Meda Yi-Ping Fu Hyesik Kong Moon Kyoo Jang Temesgen Andargie Woojin Park Jae Lee Ilker Tunc Gerald J Berry Charles C Marboe Pali D Shah Steven D Nathan Michael B Keller Sean Agbor-Enoh |
| author_facet | Rohan Meda Yi-Ping Fu Hyesik Kong Moon Kyoo Jang Temesgen Andargie Woojin Park Jae Lee Ilker Tunc Gerald J Berry Charles C Marboe Pali D Shah Steven D Nathan Michael B Keller Sean Agbor-Enoh |
| author_sort | Rohan Meda |
| collection | DOAJ |
| description | Objectives/Goals: Lung transplant is a life-saving surgery for patients with advanced lung diseases yet long-term survival remains poor. The clinical features and lung injury patterns of lung transplant recipients who die early versus those who survive longer term remain undefined. Here, we use cell-free DNA and rejection parameters to help elucidate this further. Methods/Study Population: Lung transplant candidacy prioritizes patients who have a high mortality risk within 2 years and will likely survive beyond 5 years. We stratified patients who died within 2 years of transplant as early death (n = 50) and those who survived past 5 years as long-term survivors (n = 53). Lung transplant recipients had serial blood collected as part of two prospective cohort studies. Cell-free DNA (cfDNA) was quantified using relative (% donor-derived cfDNA {%ddcfDNA}) and absolute (nuclear-derived {n-cfDNA}, mitochondrial-derived {mt-cfDNA}) measurements. As part of routine posttransplant clinical care, all patients underwent pulmonary function testing (PFT), surveillance bronchoscopy with bronchoalveolar lavage (BAL), transbronchial biopsy (TBBx), and donor-specific antibody testing (DSA). Results/Anticipated Results: Over the first 2 years after transplant, the number of episodes of antibody-mediated rejection (p) Discussion/Significance of Impact: Clinically, early-death patients perform worse on routine surveillance PFTs and experience a worse degree of CLAD. These patients also have higher levels of cfDNA as quantified by n-cfDNA and mt-cfDNA. These results provide preliminary evidence that early-death patients have worse allograft rejection, dysfunction, and molecular injury. |
| format | Article |
| id | doaj-art-eb3a290586be4a8882b5ebf83c9f8399 |
| institution | Kabale University |
| issn | 2059-8661 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj-art-eb3a290586be4a8882b5ebf83c9f83992025-08-20T03:40:54ZengCambridge University PressJournal of Clinical and Translational Science2059-86612025-04-01915815810.1017/cts.2024.1114542 The association between cell-free DNA and lung transplant survivalRohan Meda0Yi-Ping Fu1Hyesik Kong2Moon Kyoo Jang3Temesgen Andargie4Woojin Park5Jae Lee6Ilker Tunc7Gerald J Berry8Charles C Marboe9Pali D Shah10Steven D Nathan11Michael B Keller12Sean Agbor-Enoh13Georgetown University School of MedicineNational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBINational Heart, Lung, and Blood Institute-NHLBIStanford University School of MedicineNew York Presbyterian University Hospital of Cornell and ColumbiaThe Johns Hopkins School of MedicineInova Fairfax HospitalNational Heart, Lung, and Blood Institute-NHLBISenior Author/PI, NHLBIObjectives/Goals: Lung transplant is a life-saving surgery for patients with advanced lung diseases yet long-term survival remains poor. The clinical features and lung injury patterns of lung transplant recipients who die early versus those who survive longer term remain undefined. Here, we use cell-free DNA and rejection parameters to help elucidate this further. Methods/Study Population: Lung transplant candidacy prioritizes patients who have a high mortality risk within 2 years and will likely survive beyond 5 years. We stratified patients who died within 2 years of transplant as early death (n = 50) and those who survived past 5 years as long-term survivors (n = 53). Lung transplant recipients had serial blood collected as part of two prospective cohort studies. Cell-free DNA (cfDNA) was quantified using relative (% donor-derived cfDNA {%ddcfDNA}) and absolute (nuclear-derived {n-cfDNA}, mitochondrial-derived {mt-cfDNA}) measurements. As part of routine posttransplant clinical care, all patients underwent pulmonary function testing (PFT), surveillance bronchoscopy with bronchoalveolar lavage (BAL), transbronchial biopsy (TBBx), and donor-specific antibody testing (DSA). Results/Anticipated Results: Over the first 2 years after transplant, the number of episodes of antibody-mediated rejection (p) Discussion/Significance of Impact: Clinically, early-death patients perform worse on routine surveillance PFTs and experience a worse degree of CLAD. These patients also have higher levels of cfDNA as quantified by n-cfDNA and mt-cfDNA. These results provide preliminary evidence that early-death patients have worse allograft rejection, dysfunction, and molecular injury.https://www.cambridge.org/core/product/identifier/S2059866124011142/type/journal_article |
| spellingShingle | Rohan Meda Yi-Ping Fu Hyesik Kong Moon Kyoo Jang Temesgen Andargie Woojin Park Jae Lee Ilker Tunc Gerald J Berry Charles C Marboe Pali D Shah Steven D Nathan Michael B Keller Sean Agbor-Enoh 542 The association between cell-free DNA and lung transplant survival Journal of Clinical and Translational Science |
| title | 542 The association between cell-free DNA and lung transplant survival |
| title_full | 542 The association between cell-free DNA and lung transplant survival |
| title_fullStr | 542 The association between cell-free DNA and lung transplant survival |
| title_full_unstemmed | 542 The association between cell-free DNA and lung transplant survival |
| title_short | 542 The association between cell-free DNA and lung transplant survival |
| title_sort | 542 the association between cell free dna and lung transplant survival |
| url | https://www.cambridge.org/core/product/identifier/S2059866124011142/type/journal_article |
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