Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption
Poultry molting is a natural phenomenon, and process that improves the physiological function of laying hens. In this study, artificial intervention was used to induced molting (IM) in aged hens and improve their egg- laying performance. Jejunal lipophagy and lipid metabolism data were analyzed to e...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012197 |
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| author | Meiwei Lv Chenxin Liu Xiaowen Jiang Ming Ge Haibin Wang Wenhui Yu |
| author_facet | Meiwei Lv Chenxin Liu Xiaowen Jiang Ming Ge Haibin Wang Wenhui Yu |
| author_sort | Meiwei Lv |
| collection | DOAJ |
| description | Poultry molting is a natural phenomenon, and process that improves the physiological function of laying hens. In this study, artificial intervention was used to induced molting (IM) in aged hens and improve their egg- laying performance. Jejunal lipophagy and lipid metabolism data were analyzed to elucidate the regulatory mechanisms by which the intestine affects egg production performance, particularly through the lens of digestion and absorption processes. Molting process in laying hens facilitated the regeneration of small intestinal villi following damage and shedding, while also reducing excess lipid accumulation within the intestine. Analyses of lipophagy and lipid metabolism-related factors revealed, increased the expression levels of genes and proteins, such as AMPK, FOXO1, TFEB, TFE3, PGC-1α and PPAR-α (P<0.05, P<0.01 and P<0.001). Serological analysis and detection of enzymes involved in digestion and absorption, showed upregulated expression of GLUT2, FABP (P<0.05 and P<0.001) and CD36 (P<0.01), and the activities of amylase, chymotrypsin and Lipase also increased significantly (P<0.05, P<0.01 and P<0.001). In conclusion, artificially IM activates the AMPK-lipophagy-lipid metabolism pathway to enhance intestinal digestion and absorption in laying hens. Our findings offer a theoretical framework for the intentional use of IM to promote a healthy state of digestion and absorption. |
| format | Article |
| id | doaj-art-eb33a3fa8996498b8c4b92d504126cb7 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-eb33a3fa8996498b8c4b92d504126cb72025-01-22T05:40:48ZengElsevierPoultry Science0032-57912025-01-011041104641Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorptionMeiwei Lv0Chenxin Liu1Xiaowen Jiang2Ming Ge3Haibin Wang4Wenhui Yu5College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Provincial Key Laboratory of Comparative Medicine and Animal Pathogenesis, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Institute of Traditional Chinese Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Institute of Traditional Chinese Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Institute of Traditional Chinese Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Corresponding author: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.Poultry molting is a natural phenomenon, and process that improves the physiological function of laying hens. In this study, artificial intervention was used to induced molting (IM) in aged hens and improve their egg- laying performance. Jejunal lipophagy and lipid metabolism data were analyzed to elucidate the regulatory mechanisms by which the intestine affects egg production performance, particularly through the lens of digestion and absorption processes. Molting process in laying hens facilitated the regeneration of small intestinal villi following damage and shedding, while also reducing excess lipid accumulation within the intestine. Analyses of lipophagy and lipid metabolism-related factors revealed, increased the expression levels of genes and proteins, such as AMPK, FOXO1, TFEB, TFE3, PGC-1α and PPAR-α (P<0.05, P<0.01 and P<0.001). Serological analysis and detection of enzymes involved in digestion and absorption, showed upregulated expression of GLUT2, FABP (P<0.05 and P<0.001) and CD36 (P<0.01), and the activities of amylase, chymotrypsin and Lipase also increased significantly (P<0.05, P<0.01 and P<0.001). In conclusion, artificially IM activates the AMPK-lipophagy-lipid metabolism pathway to enhance intestinal digestion and absorption in laying hens. Our findings offer a theoretical framework for the intentional use of IM to promote a healthy state of digestion and absorption.http://www.sciencedirect.com/science/article/pii/S0032579124012197Induced moltingAMPKLipophagyLipid metabolismDigestion and absorption |
| spellingShingle | Meiwei Lv Chenxin Liu Xiaowen Jiang Ming Ge Haibin Wang Wenhui Yu Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption Poultry Science Induced molting AMPK Lipophagy Lipid metabolism Digestion and absorption |
| title | Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption |
| title_full | Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption |
| title_fullStr | Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption |
| title_full_unstemmed | Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption |
| title_short | Molting of laying hens can activate AMPK- lipophagy - lipid metabolism pathway and improve intestinal digestion and absorption |
| title_sort | molting of laying hens can activate ampk lipophagy lipid metabolism pathway and improve intestinal digestion and absorption |
| topic | Induced molting AMPK Lipophagy Lipid metabolism Digestion and absorption |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012197 |
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