Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses.
Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of m...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-08-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0002966&type=printable |
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| author | Vincent Plagnol Elif Uz Chris Wallace Helen Stevens David Clayton Tayfun Ozcelik John A Todd |
| author_facet | Vincent Plagnol Elif Uz Chris Wallace Helen Stevens David Clayton Tayfun Ozcelik John A Todd |
| author_sort | Vincent Plagnol |
| collection | DOAJ |
| description | Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays. |
| format | Article |
| id | doaj-art-eb2ec1a3a1f24a98a4e28eee1433ebfe |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2008-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-eb2ec1a3a1f24a98a4e28eee1433ebfe2025-08-20T02:00:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-08-0138e296610.1371/journal.pone.0002966Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses.Vincent PlagnolElif UzChris WallaceHelen StevensDavid ClaytonTayfun OzcelikJohn A ToddLymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0002966&type=printable |
| spellingShingle | Vincent Plagnol Elif Uz Chris Wallace Helen Stevens David Clayton Tayfun Ozcelik John A Todd Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. PLoS ONE |
| title | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. |
| title_full | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. |
| title_fullStr | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. |
| title_full_unstemmed | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. |
| title_short | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses. |
| title_sort | extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0002966&type=printable |
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