Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs
Studies have shown that the development of myopia is associated with scleral remodeling, but the exact mechanism is not yet clear. This study investigates the effects of vitreous injection of recombinant human bone morphogenetic protein 2 (rhBMP2) on scleral remodeling in myopic guinea pigs and the...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1526656/full |
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| author | Yijie Liu Qi Hao Xuemei Pan Pubo Wang Dadong Guo Qingmei Tian Xiuyan Zhang Xiuzhen Lu Qiuxin Wu Hongsheng Bi |
| author_facet | Yijie Liu Qi Hao Xuemei Pan Pubo Wang Dadong Guo Qingmei Tian Xiuyan Zhang Xiuzhen Lu Qiuxin Wu Hongsheng Bi |
| author_sort | Yijie Liu |
| collection | DOAJ |
| description | Studies have shown that the development of myopia is associated with scleral remodeling, but the exact mechanism is not yet clear. This study investigates the effects of vitreous injection of recombinant human bone morphogenetic protein 2 (rhBMP2) on scleral remodeling in myopic guinea pigs and the possible signaling pathways. Guinea pigs were randomly divided into normal control (NC) group, lens-induced myopia (LIM) group, rhBMP2 low-dose group (LD), rhBMP2 medium-dose group (MD), and rhBMP2 high-dose group (HD). After rhBMP2 intervention, myopic refraction was reduced and axial growth was delayed compared with the LIM group; Hematoxylin–eosin (H&E) staining showed that the arrangement of scleral collagen fibers was loose, the disorder was improved, and the cavities were reduced, especially in MD group; and immunofluorescence staining showed elevated α-SMA protein expression. Q-PCR and western blot results showed that after rhBMP2 intervention, at the mRNA and protein levels, the expression of BMPRIA, smad1, smad5, smad9, smad4, TIMP2, and Col1A1 was up-regulated, and MMP2 expression was down-regulated when compared with the LIM group. From this study, we conclude that after injecting rhBMP2 into the vitreous cavity of experimental myopic guinea pigs, it can bind to BMP2-related receptors, activate smad signaling pathway, affect the expression of MMP2/TIMP2, promote the expression of Col1A1 gene, regulate scleral remodeling, promote collagen I synthesis, and delay the development of myopia. |
| format | Article |
| id | doaj-art-eb26e10e246b487ca2ce006c938477fb |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-eb26e10e246b487ca2ce006c938477fb2025-08-20T02:55:39ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-05-011210.3389/fmed.2025.15266561526656Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigsYijie Liu0Qi Hao1Xuemei Pan2Pubo Wang3Dadong Guo4Qingmei Tian5Xiuyan Zhang6Xiuzhen Lu7Qiuxin Wu8Hongsheng Bi9Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaStudies have shown that the development of myopia is associated with scleral remodeling, but the exact mechanism is not yet clear. This study investigates the effects of vitreous injection of recombinant human bone morphogenetic protein 2 (rhBMP2) on scleral remodeling in myopic guinea pigs and the possible signaling pathways. Guinea pigs were randomly divided into normal control (NC) group, lens-induced myopia (LIM) group, rhBMP2 low-dose group (LD), rhBMP2 medium-dose group (MD), and rhBMP2 high-dose group (HD). After rhBMP2 intervention, myopic refraction was reduced and axial growth was delayed compared with the LIM group; Hematoxylin–eosin (H&E) staining showed that the arrangement of scleral collagen fibers was loose, the disorder was improved, and the cavities were reduced, especially in MD group; and immunofluorescence staining showed elevated α-SMA protein expression. Q-PCR and western blot results showed that after rhBMP2 intervention, at the mRNA and protein levels, the expression of BMPRIA, smad1, smad5, smad9, smad4, TIMP2, and Col1A1 was up-regulated, and MMP2 expression was down-regulated when compared with the LIM group. From this study, we conclude that after injecting rhBMP2 into the vitreous cavity of experimental myopic guinea pigs, it can bind to BMP2-related receptors, activate smad signaling pathway, affect the expression of MMP2/TIMP2, promote the expression of Col1A1 gene, regulate scleral remodeling, promote collagen I synthesis, and delay the development of myopia.https://www.frontiersin.org/articles/10.3389/fmed.2025.1526656/fullmyopiarecombinant human bone morphogenetic protein 2BMP/smadscleral remodelingscleral |
| spellingShingle | Yijie Liu Qi Hao Xuemei Pan Pubo Wang Dadong Guo Qingmei Tian Xiuyan Zhang Xiuzhen Lu Qiuxin Wu Hongsheng Bi Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs Frontiers in Medicine myopia recombinant human bone morphogenetic protein 2 BMP/smad scleral remodeling scleral |
| title | Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs |
| title_full | Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs |
| title_fullStr | Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs |
| title_full_unstemmed | Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs |
| title_short | Harnessing the potential of recombinant human BMP2: regulating scleral changes in myopic guinea pigs |
| title_sort | harnessing the potential of recombinant human bmp2 regulating scleral changes in myopic guinea pigs |
| topic | myopia recombinant human bone morphogenetic protein 2 BMP/smad scleral remodeling scleral |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1526656/full |
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