Effect of Seminal Plasma on the Freezability of Boar Sperm
Background: Seminal plasma is an important component of semen and has a significant effect on sperm function. However, the relationship between seminal plasma and sperm freezing capacity has not been fully studied. Purpose: Exploring metabolites and proteins related to the boar sperm freezing capaci...
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MDPI AG
2024-12-01
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| Series: | Animals |
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| author | Kuanfeng Zhu Yukun Song Zhi He Peng Wang Xuguang Wang Guoshi Liu |
| author_facet | Kuanfeng Zhu Yukun Song Zhi He Peng Wang Xuguang Wang Guoshi Liu |
| author_sort | Kuanfeng Zhu |
| collection | DOAJ |
| description | Background: Seminal plasma is an important component of semen and has a significant effect on sperm function. However, the relationship between seminal plasma and sperm freezing capacity has not been fully studied. Purpose: Exploring metabolites and proteins related to the boar sperm freezing capacity in seminal plasma, by metabolomic and proteomic approaches, and directly verifying the protective effect of seminal plasma on the cryopreservation of boar sperm using high and low freezability seminal plasma as base freezing extender. Methods: Semen samples were collected from 30 different boars, 11 high and 11 low freezing-resistant boars were selected after freezing 2~4 times, and seminal plasma was selected at the same time. Sperm motility and movement parameters were analyzed using a CASA system. Reproductive hormones (Testosterone, progesterone, estradiol, prolactin, prostaglandin F2α, luteinoid hormone) in seminal plasma were detected by ELISA. Analysis of proteins and metabolites in high and low freezing-resistant seminal plasma by proteomics and metabolomics techniques. Results: The six reproductive hormones tested were not significantly associated with sperm freezing resistance. A total of 13 differentially expressed metabolites (DEMs) and 38 differentially expressed proteins (DEPs) were identified, while a total of 348 metabolites and 1000 proteins were identified. These DEMs were related to energy metabolism, drugs, or environmental pollutants, while the DEPs were mainly involved in the cytoskeletal dynamics and cell adhesion processes. There were 33 metabolites and 70 proteins significantly associated with mean progress motility (PM) at 10 min and 2 h after thawing. The 70 related proteins were associated with cell division and cycle regulation in gene ontology (GO) terms, as well as KEGG pathways, thermogeneration, and pyruvate metabolism. Using highly freezable boar SP as a base freezing extender made no difference from using lowly freezable boar SP, and both were not as good as the commercial control. Conclusion: There were significant differences in seminal plasma with different freezability, but the similarity was much greater than the difference. The protection effect of seminal plasma is not remarkable, and it does not exhibit superior cryoprotective properties compared to commercial semen cryoelongators. Significance: This study provides a deeper understanding of how seminal plasma composition affects sperm freezabilty. It provides potential biomarkers and targets for improving sperm cryopreservation techniques. |
| format | Article |
| id | doaj-art-eb246d1237124b3496fa37eadd2ead9f |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-eb246d1237124b3496fa37eadd2ead9f2025-08-20T02:24:39ZengMDPI AGAnimals2076-26152024-12-011424365610.3390/ani14243656Effect of Seminal Plasma on the Freezability of Boar SpermKuanfeng Zhu0Yukun Song1Zhi He2Peng Wang3Xuguang Wang4Guoshi Liu5College of Animal Science, Xinjiang Agricultural University, Urumqi 830091, ChinaBeijing Jingwa Agricultural Science & Technology Innovation Center, Beijing 101205, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830091, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830091, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi 830091, ChinaBackground: Seminal plasma is an important component of semen and has a significant effect on sperm function. However, the relationship between seminal plasma and sperm freezing capacity has not been fully studied. Purpose: Exploring metabolites and proteins related to the boar sperm freezing capacity in seminal plasma, by metabolomic and proteomic approaches, and directly verifying the protective effect of seminal plasma on the cryopreservation of boar sperm using high and low freezability seminal plasma as base freezing extender. Methods: Semen samples were collected from 30 different boars, 11 high and 11 low freezing-resistant boars were selected after freezing 2~4 times, and seminal plasma was selected at the same time. Sperm motility and movement parameters were analyzed using a CASA system. Reproductive hormones (Testosterone, progesterone, estradiol, prolactin, prostaglandin F2α, luteinoid hormone) in seminal plasma were detected by ELISA. Analysis of proteins and metabolites in high and low freezing-resistant seminal plasma by proteomics and metabolomics techniques. Results: The six reproductive hormones tested were not significantly associated with sperm freezing resistance. A total of 13 differentially expressed metabolites (DEMs) and 38 differentially expressed proteins (DEPs) were identified, while a total of 348 metabolites and 1000 proteins were identified. These DEMs were related to energy metabolism, drugs, or environmental pollutants, while the DEPs were mainly involved in the cytoskeletal dynamics and cell adhesion processes. There were 33 metabolites and 70 proteins significantly associated with mean progress motility (PM) at 10 min and 2 h after thawing. The 70 related proteins were associated with cell division and cycle regulation in gene ontology (GO) terms, as well as KEGG pathways, thermogeneration, and pyruvate metabolism. Using highly freezable boar SP as a base freezing extender made no difference from using lowly freezable boar SP, and both were not as good as the commercial control. Conclusion: There were significant differences in seminal plasma with different freezability, but the similarity was much greater than the difference. The protection effect of seminal plasma is not remarkable, and it does not exhibit superior cryoprotective properties compared to commercial semen cryoelongators. Significance: This study provides a deeper understanding of how seminal plasma composition affects sperm freezabilty. It provides potential biomarkers and targets for improving sperm cryopreservation techniques.https://www.mdpi.com/2076-2615/14/24/3656seminal plasmaboarproteomemetabolomefreezability |
| spellingShingle | Kuanfeng Zhu Yukun Song Zhi He Peng Wang Xuguang Wang Guoshi Liu Effect of Seminal Plasma on the Freezability of Boar Sperm Animals seminal plasma boar proteome metabolome freezability |
| title | Effect of Seminal Plasma on the Freezability of Boar Sperm |
| title_full | Effect of Seminal Plasma on the Freezability of Boar Sperm |
| title_fullStr | Effect of Seminal Plasma on the Freezability of Boar Sperm |
| title_full_unstemmed | Effect of Seminal Plasma on the Freezability of Boar Sperm |
| title_short | Effect of Seminal Plasma on the Freezability of Boar Sperm |
| title_sort | effect of seminal plasma on the freezability of boar sperm |
| topic | seminal plasma boar proteome metabolome freezability |
| url | https://www.mdpi.com/2076-2615/14/24/3656 |
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