Knockout of TMEM206 in mice associated with a loss of corneal transparency
AIM: To investigate the role of transmembrane protein 206 (TMEM206) in corneal edema in mice by knockout the TMEM206 gene using CRISPR/Cas9 editing technology. METHODS: TMEM206-knockout mice were generated using the CRISPR-Cas9 system. Variations in ophthalmic pathology were observed using slit lamp...
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Press of International Journal of Ophthalmology (IJO PRESS)
2024-11-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2024/11/20241101.pdf |
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| author | Zi-Jian Yang Shou-Yue Huang Yu-Feng Zhou Shun-Chang Sun |
| author_facet | Zi-Jian Yang Shou-Yue Huang Yu-Feng Zhou Shun-Chang Sun |
| author_sort | Zi-Jian Yang |
| collection | DOAJ |
| description | AIM: To investigate the role of transmembrane protein 206 (TMEM206) in corneal edema in mice by knockout the TMEM206 gene using CRISPR/Cas9 editing technology. METHODS: TMEM206-knockout mice were generated using the CRISPR-Cas9 system. Variations in ophthalmic pathology were observed using slit lamp microscope and optical coherence tomography (OCT), intraocular pressure (IOP) was measured using a TonoLab Rebound Tonometer, and the ultrastructure of the corneal was observed using a transmission electron microscope. RESULTS: Corneal opacity was observed in 4/18 homozygous TMEM206-/- mice whereas a similar change was not observed in heterozygous TMEM206+/- mice and wild-type littermates. OCT examination showed that the mean central cornea thickness was 125±5.4 µm in 4 homozygous TMEM206-/- mice developed corneal edema and 115±1.2 µm in wild-type mice (t=3.468, P<0.05) at 43wk. The mean IOP was 12.08±0.07 mm Hg in four right eyes with corneal edema and 12.03±0.03 mm Hg in three normal left eyes (P>0.05). Transmission electron microscopy revealed a disruption in the organization of the collagen fibrils in the central part of the cornea in homozygous TMEM206-/- mice. CONCLUSION: TMEM206 is associated with corneal edema which caused organizational disruption of collagen fibrils in corneas of mice. |
| format | Article |
| id | doaj-art-eb22e0777d934c80976d0fc007fd0000 |
| institution | OA Journals |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
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| series | International Journal of Ophthalmology |
| spelling | doaj-art-eb22e0777d934c80976d0fc007fd00002025-08-20T02:09:48ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982024-11-0117111967197210.18240/ijo.2024.11.0120241101Knockout of TMEM206 in mice associated with a loss of corneal transparencyZi-Jian Yang0Shou-Yue Huang1Yu-Feng Zhou2Shun-Chang Sun3Shun-Chang Sun. Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, China. shunchangsun@aliyun.comDepartment of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, ChinaDepartment of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, ChinaDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, ChinaAIM: To investigate the role of transmembrane protein 206 (TMEM206) in corneal edema in mice by knockout the TMEM206 gene using CRISPR/Cas9 editing technology. METHODS: TMEM206-knockout mice were generated using the CRISPR-Cas9 system. Variations in ophthalmic pathology were observed using slit lamp microscope and optical coherence tomography (OCT), intraocular pressure (IOP) was measured using a TonoLab Rebound Tonometer, and the ultrastructure of the corneal was observed using a transmission electron microscope. RESULTS: Corneal opacity was observed in 4/18 homozygous TMEM206-/- mice whereas a similar change was not observed in heterozygous TMEM206+/- mice and wild-type littermates. OCT examination showed that the mean central cornea thickness was 125±5.4 µm in 4 homozygous TMEM206-/- mice developed corneal edema and 115±1.2 µm in wild-type mice (t=3.468, P<0.05) at 43wk. The mean IOP was 12.08±0.07 mm Hg in four right eyes with corneal edema and 12.03±0.03 mm Hg in three normal left eyes (P>0.05). Transmission electron microscopy revealed a disruption in the organization of the collagen fibrils in the central part of the cornea in homozygous TMEM206-/- mice. CONCLUSION: TMEM206 is associated with corneal edema which caused organizational disruption of collagen fibrils in corneas of mice.http://ies.ijo.cn/en_publish/2024/11/20241101.pdftransmembrane protein 206knockoutcorneaedemamouse |
| spellingShingle | Zi-Jian Yang Shou-Yue Huang Yu-Feng Zhou Shun-Chang Sun Knockout of TMEM206 in mice associated with a loss of corneal transparency International Journal of Ophthalmology transmembrane protein 206 knockout cornea edema mouse |
| title | Knockout of TMEM206 in mice associated with a loss of corneal transparency |
| title_full | Knockout of TMEM206 in mice associated with a loss of corneal transparency |
| title_fullStr | Knockout of TMEM206 in mice associated with a loss of corneal transparency |
| title_full_unstemmed | Knockout of TMEM206 in mice associated with a loss of corneal transparency |
| title_short | Knockout of TMEM206 in mice associated with a loss of corneal transparency |
| title_sort | knockout of tmem206 in mice associated with a loss of corneal transparency |
| topic | transmembrane protein 206 knockout cornea edema mouse |
| url | http://ies.ijo.cn/en_publish/2024/11/20241101.pdf |
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