Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock
One of the principal limitations on livestock productivity in sub-Saharan Africa is the constraining effect of infectious diseases, including tick-borne blood pathogens. Currently, diagnostic markers for these pathogens are species or genus specific, making it challenging to implement high-throughpu...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1491828/full |
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| author | Erhan Yalcindag Erhan Yalcindag Deepali Vasoya Johanneke D. Hemmink Johanneke D. Hemmink Johanneke D. Hemmink Benedict Karani Benedict Karani Luis Enrique Hernandez Castro Luis Enrique Hernandez Castro Rebecca Callaby Rebecca Callaby Stella Mazeri Edith Paxton Timothy K. Connelley Timothy K. Connelley Phil Toye Phil Toye Liam J. Morrison Liam J. Morrison Barend Mark de C. Bronsvoort Barend Mark de C. Bronsvoort |
| author_facet | Erhan Yalcindag Erhan Yalcindag Deepali Vasoya Johanneke D. Hemmink Johanneke D. Hemmink Johanneke D. Hemmink Benedict Karani Benedict Karani Luis Enrique Hernandez Castro Luis Enrique Hernandez Castro Rebecca Callaby Rebecca Callaby Stella Mazeri Edith Paxton Timothy K. Connelley Timothy K. Connelley Phil Toye Phil Toye Liam J. Morrison Liam J. Morrison Barend Mark de C. Bronsvoort Barend Mark de C. Bronsvoort |
| author_sort | Erhan Yalcindag |
| collection | DOAJ |
| description | One of the principal limitations on livestock productivity in sub-Saharan Africa is the constraining effect of infectious diseases, including tick-borne blood pathogens. Currently, diagnostic markers for these pathogens are species or genus specific, making it challenging to implement high-throughput screening methods. The aim of this study was to develop and validate a novel high-throughput diagnostic tool capable of detecting a range of important haemopathogens in livestock. To achieve this, we developed a high-throughput diagnostic tool that can detect all species of Anaplasma, Ehrlichia, Theileria and Babesia present in a sample. The approach involves targeting the 16S/18S rDNA region by PCR and subjecting amplicons to deep sequencing, which allows for the identification of species present in a sample, and the exploration of haemopathogen communities. To validate the accuracy of this Next Generation Sequencing method, we compared the amplicon sequencing results with species-specific PCR and reverse line blot (RLB) test data of both control and field samples. The Haemabiome tool demonstrated the successful resolution of positive and negative samples, and highlighted the power of this diagnostic tool in identifying multiplicity of infections. The Haemabiome tool can therefore generate valuable insights regarding the understanding of the true diversity of species composition and the distribution of pathogen communities in field samples. |
| format | Article |
| id | doaj-art-eb1e224915e640d49da030a4e0182e6e |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-eb1e224915e640d49da030a4e0182e6e2025-08-20T01:58:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14918281491828Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestockErhan Yalcindag0Erhan Yalcindag1Deepali Vasoya2Johanneke D. Hemmink3Johanneke D. Hemmink4Johanneke D. Hemmink5Benedict Karani6Benedict Karani7Luis Enrique Hernandez Castro8Luis Enrique Hernandez Castro9Rebecca Callaby10Rebecca Callaby11Stella Mazeri12Edith Paxton13Timothy K. Connelley14Timothy K. Connelley15Phil Toye16Phil Toye17Liam J. Morrison18Liam J. Morrison19Barend Mark de C. Bronsvoort20Barend Mark de C. Bronsvoort21Centre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), ILRI Kenya, Nairobi, KenyaThe International Livestock Research Institute, Nairobi, KenyaCentre for Tropical Livestock Genetics and Health (CTLGH), ILRI Kenya, Nairobi, KenyaThe International Livestock Research Institute, Nairobi, KenyaCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), ILRI Kenya, Nairobi, KenyaThe International Livestock Research Institute, Nairobi, KenyaCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomOne of the principal limitations on livestock productivity in sub-Saharan Africa is the constraining effect of infectious diseases, including tick-borne blood pathogens. Currently, diagnostic markers for these pathogens are species or genus specific, making it challenging to implement high-throughput screening methods. The aim of this study was to develop and validate a novel high-throughput diagnostic tool capable of detecting a range of important haemopathogens in livestock. To achieve this, we developed a high-throughput diagnostic tool that can detect all species of Anaplasma, Ehrlichia, Theileria and Babesia present in a sample. The approach involves targeting the 16S/18S rDNA region by PCR and subjecting amplicons to deep sequencing, which allows for the identification of species present in a sample, and the exploration of haemopathogen communities. To validate the accuracy of this Next Generation Sequencing method, we compared the amplicon sequencing results with species-specific PCR and reverse line blot (RLB) test data of both control and field samples. The Haemabiome tool demonstrated the successful resolution of positive and negative samples, and highlighted the power of this diagnostic tool in identifying multiplicity of infections. The Haemabiome tool can therefore generate valuable insights regarding the understanding of the true diversity of species composition and the distribution of pathogen communities in field samples.https://www.frontiersin.org/articles/10.3389/fvets.2024.1491828/fullhigh-throughput amplicon sequencingmolecular diagnosishaemopathogenscattleAfrica |
| spellingShingle | Erhan Yalcindag Erhan Yalcindag Deepali Vasoya Johanneke D. Hemmink Johanneke D. Hemmink Johanneke D. Hemmink Benedict Karani Benedict Karani Luis Enrique Hernandez Castro Luis Enrique Hernandez Castro Rebecca Callaby Rebecca Callaby Stella Mazeri Edith Paxton Timothy K. Connelley Timothy K. Connelley Phil Toye Phil Toye Liam J. Morrison Liam J. Morrison Barend Mark de C. Bronsvoort Barend Mark de C. Bronsvoort Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock Frontiers in Veterinary Science high-throughput amplicon sequencing molecular diagnosis haemopathogens cattle Africa |
| title | Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock |
| title_full | Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock |
| title_fullStr | Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock |
| title_full_unstemmed | Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock |
| title_short | Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock |
| title_sort | development of a novel haemabiome tool for the high throughput analysis of haemopathogen species co infections in african livestock |
| topic | high-throughput amplicon sequencing molecular diagnosis haemopathogens cattle Africa |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1491828/full |
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