Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro
The extracellular matrix (ECM) forms the basis of every phase in wound healing. Healing may be impaired if some of these components are destroyed. Photobiostimulation has demonstrated a stimulatory response in biological processes. This study aimed to evaluate various genes involved in the ECM, in r...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/604518 |
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| _version_ | 1832548985302155264 |
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| author | Sandra M. Ayuk Nicolette N. Houreld Heidi Abrahamse |
| author_facet | Sandra M. Ayuk Nicolette N. Houreld Heidi Abrahamse |
| author_sort | Sandra M. Ayuk |
| collection | DOAJ |
| description | The extracellular matrix (ECM) forms the basis of every phase in wound healing. Healing may be impaired if some of these components are destroyed. Photobiostimulation has demonstrated a stimulatory response in biological processes. This study aimed to evaluate various genes involved in the ECM, in response to laser irradiation. Isolated human skin fibroblasts were used in three different cell models, namely, normal, normal wounded, and diabetic wounded. Cells were irradiated with 5 J/cm2 using a continuous wave diode laser emitting at a wavelength of 660 nm and incubated for 48 h. Nonirradiated (0 J/cm2) normal and diabetic wounded cells served as the control. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of 84 genes in a PCR array. There was a significant upregulation of 29 genes in the normal cells, 32 genes in the normal wounded cells, and 18 genes in the diabetic wounded cells as well as a downregulation of 19 genes (normal), 6 genes (normal wounded), and 31 genes (diabetic wounded). Low intensity laser irradiation (LILI) stimulates gene expression in various cell adhesion molecules (CAMs) and extracellular proteins at 660 nm in wounded fibroblasts in vitro. |
| format | Article |
| id | doaj-art-eb088ff6f5ad4e3889aa7c9fe959d9e1 |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-eb088ff6f5ad4e3889aa7c9fe959d9e12025-02-03T06:12:32ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/604518604518Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In VitroSandra M. Ayuk0Nicolette N. Houreld1Heidi Abrahamse2Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaThe extracellular matrix (ECM) forms the basis of every phase in wound healing. Healing may be impaired if some of these components are destroyed. Photobiostimulation has demonstrated a stimulatory response in biological processes. This study aimed to evaluate various genes involved in the ECM, in response to laser irradiation. Isolated human skin fibroblasts were used in three different cell models, namely, normal, normal wounded, and diabetic wounded. Cells were irradiated with 5 J/cm2 using a continuous wave diode laser emitting at a wavelength of 660 nm and incubated for 48 h. Nonirradiated (0 J/cm2) normal and diabetic wounded cells served as the control. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of 84 genes in a PCR array. There was a significant upregulation of 29 genes in the normal cells, 32 genes in the normal wounded cells, and 18 genes in the diabetic wounded cells as well as a downregulation of 19 genes (normal), 6 genes (normal wounded), and 31 genes (diabetic wounded). Low intensity laser irradiation (LILI) stimulates gene expression in various cell adhesion molecules (CAMs) and extracellular proteins at 660 nm in wounded fibroblasts in vitro.http://dx.doi.org/10.1155/2014/604518 |
| spellingShingle | Sandra M. Ayuk Nicolette N. Houreld Heidi Abrahamse Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro International Journal of Photoenergy |
| title | Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro |
| title_full | Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro |
| title_fullStr | Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro |
| title_full_unstemmed | Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro |
| title_short | Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro |
| title_sort | laser irradiation alters the expression profile of genes involved in the extracellular matrix in vitro |
| url | http://dx.doi.org/10.1155/2014/604518 |
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