Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran
ABSTRACT Background Hereditary hearing loss (HHL) is a clinically and genetically heterogeneous sensorineural disorder that presents challenges for diagnosis. Next‐generation sequencing (NGS) approaches have facilitated a more cost‐effective, streamlined diagnostic process. This study aimed to ident...
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Wiley
2025-02-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.70080 |
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| author | Marzieh Mohseni Farzane Zare Ashrafi Ehsan Abbaspour Rodbaneh Haleh Mokabber Maryam Vafaei Ramiz Nobakht Fatemeh Keshavarzi Sanaz Arzhangi Sara Arish Zahra Nematollahi Azar Kimia Kahrizi Hossein Najmabadi Behzad Davarnia |
| author_facet | Marzieh Mohseni Farzane Zare Ashrafi Ehsan Abbaspour Rodbaneh Haleh Mokabber Maryam Vafaei Ramiz Nobakht Fatemeh Keshavarzi Sanaz Arzhangi Sara Arish Zahra Nematollahi Azar Kimia Kahrizi Hossein Najmabadi Behzad Davarnia |
| author_sort | Marzieh Mohseni |
| collection | DOAJ |
| description | ABSTRACT Background Hereditary hearing loss (HHL) is a clinically and genetically heterogeneous sensorineural disorder that presents challenges for diagnosis. Next‐generation sequencing (NGS) approaches have facilitated a more cost‐effective, streamlined diagnostic process. This study aimed to identify HHL variants using NGS in Iranian Azeri families in Ardabil Province, establishing a suitable framework for screening programs tailored to the local population. Methods Seventy‐four GJB2‐negative Azeri families with HHL from Ardabil Province of Iran were studied using the OtoSCOPE panel and/or exome sequencing over 15‐years from 2008 to 2023. Results Data analysis revealed 53 HHL variants in 52 of the 74 most consanguineous families (70%), including 34 pathogenic/likely pathogenic variants and 19 variants of uncertain significance. Seventeen of the detected variants were novel. SLC26A4, MYO7A, USH2A, and TMPRSS3 were the most prevalent mutated genes for non‐syndromic hearing loss (NSHL) and syndromic hearing loss (SHL) in this study. Conclusion Our results are comparable to those of previous studies, indicating that SLC26A4 is the second most common cause of HHL, after GJB2. Moreover, our study emphasizes the significance of understanding the genetic basis of HL for early diagnosis and the implementation of suitable screening programs for various ethnicities in Iran. |
| format | Article |
| id | doaj-art-eaff819802d74198b50c779425ce7e91 |
| institution | OA Journals |
| issn | 2324-9269 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
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| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj-art-eaff819802d74198b50c779425ce7e912025-08-20T02:01:19ZengWileyMolecular Genetics & Genomic Medicine2324-92692025-02-01132n/an/a10.1002/mgg3.70080Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, IranMarzieh Mohseni0Farzane Zare Ashrafi1Ehsan Abbaspour Rodbaneh2Haleh Mokabber3Maryam Vafaei4Ramiz Nobakht5Fatemeh Keshavarzi6Sanaz Arzhangi7Sara Arish8Zahra Nematollahi Azar9Kimia Kahrizi10Hossein Najmabadi11Behzad Davarnia12Genetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranGenetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranKariminejad – Najmabadi Pathology & Genetics Center Tehran IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranGenetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranGenetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranGenetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranGenetics Research Center University of Social Welfare and Rehabilitation Sciences Tehran IranDepartment of Medical Genetics and Pathology Ardabil University of Medical Sciences Ardabil IranABSTRACT Background Hereditary hearing loss (HHL) is a clinically and genetically heterogeneous sensorineural disorder that presents challenges for diagnosis. Next‐generation sequencing (NGS) approaches have facilitated a more cost‐effective, streamlined diagnostic process. This study aimed to identify HHL variants using NGS in Iranian Azeri families in Ardabil Province, establishing a suitable framework for screening programs tailored to the local population. Methods Seventy‐four GJB2‐negative Azeri families with HHL from Ardabil Province of Iran were studied using the OtoSCOPE panel and/or exome sequencing over 15‐years from 2008 to 2023. Results Data analysis revealed 53 HHL variants in 52 of the 74 most consanguineous families (70%), including 34 pathogenic/likely pathogenic variants and 19 variants of uncertain significance. Seventeen of the detected variants were novel. SLC26A4, MYO7A, USH2A, and TMPRSS3 were the most prevalent mutated genes for non‐syndromic hearing loss (NSHL) and syndromic hearing loss (SHL) in this study. Conclusion Our results are comparable to those of previous studies, indicating that SLC26A4 is the second most common cause of HHL, after GJB2. Moreover, our study emphasizes the significance of understanding the genetic basis of HL for early diagnosis and the implementation of suitable screening programs for various ethnicities in Iran.https://doi.org/10.1002/mgg3.70080Azeri populationexome sequencinghereditary hearing lossIranOtoSCOPE |
| spellingShingle | Marzieh Mohseni Farzane Zare Ashrafi Ehsan Abbaspour Rodbaneh Haleh Mokabber Maryam Vafaei Ramiz Nobakht Fatemeh Keshavarzi Sanaz Arzhangi Sara Arish Zahra Nematollahi Azar Kimia Kahrizi Hossein Najmabadi Behzad Davarnia Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran Molecular Genetics & Genomic Medicine Azeri population exome sequencing hereditary hearing loss Iran OtoSCOPE |
| title | Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran |
| title_full | Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran |
| title_fullStr | Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran |
| title_full_unstemmed | Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran |
| title_short | Unraveling the Genetic Landscape of Hearing Loss: A Comprehensive Study of Azeri Families in Ardabil, Iran |
| title_sort | unraveling the genetic landscape of hearing loss a comprehensive study of azeri families in ardabil iran |
| topic | Azeri population exome sequencing hereditary hearing loss Iran OtoSCOPE |
| url | https://doi.org/10.1002/mgg3.70080 |
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