Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae)
The aim of this work was to evaluate the spectrophotometric methodology for determining the total flavonoid content (TFC) in herbal drug and derived products from Bauhinia monandra Kurz. Several analytical parameters from this method grounded on the complex formed between flavonoids and AlCl3 were e...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/923462 |
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| author | Ana Josane Dantas Fernandes Magda Rhayanny Assunção Ferreira Karina Perrelli Randau Tatiane Pereira de Souza Luiz Alberto Lira Soares |
| author_facet | Ana Josane Dantas Fernandes Magda Rhayanny Assunção Ferreira Karina Perrelli Randau Tatiane Pereira de Souza Luiz Alberto Lira Soares |
| author_sort | Ana Josane Dantas Fernandes |
| collection | DOAJ |
| description | The aim of this work was to evaluate the spectrophotometric methodology for determining the total flavonoid content (TFC) in herbal drug and derived products from Bauhinia monandra Kurz. Several analytical parameters from this method grounded on the complex formed between flavonoids and AlCl3 were evaluated such as herbal amount (0.25 to 1.25 g); solvent composition (ethanol 40 to 80%, v/v); as well as the reaction time and AlCl3 concentration (2 to 9%, w/v). The method was adjusted to aqueous extractives and its performance studied through precision, linearity and preliminary robustness. The results showed an important dependence of the method response from reaction time, AlCl3 concentration, sample amount, and solvent mixture. After choosing the optimized condition, the method was applied for the matrixes (herbal material and extractives), showing precision lower than 5% (for both parameters repeatability and intermediate precision), coefficient of determination higher than 0.99, and no important influence could be observed for slight variations from wavelength or AlCl3 concentration. Thus, it could be concluded that the evaluated analytical procedure was suitable to quantify the total flavonoid content in raw material and aqueous extractives from leaves of B. monandra. |
| format | Article |
| id | doaj-art-eaff499c20334edf8bbb2eb0a198b2f4 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-eaff499c20334edf8bbb2eb0a198b2f42025-02-03T06:12:07ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/923462923462Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae)Ana Josane Dantas Fernandes0Magda Rhayanny Assunção Ferreira1Karina Perrelli Randau2Tatiane Pereira de Souza3Luiz Alberto Lira Soares4Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia, UFRN, Avenida Gal. Cordeiro de Farias s/n, 59010-180 Natal, RN, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia, UFRN, Avenida Gal. Cordeiro de Farias s/n, 59010-180 Natal, RN, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Departamento de Ciências Farmacêuticas, UFPE, Rua Prof. Artur de Sá s/n, 50740-521 Recife, PE, BrazilDepartamento de Medicamentos e Alimentos, Universidade Federal do Amazonas, Rua Alexandre Amorim 330, Aparecida, 69010-300 Manaus, AM, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia, UFRN, Avenida Gal. Cordeiro de Farias s/n, 59010-180 Natal, RN, BrazilThe aim of this work was to evaluate the spectrophotometric methodology for determining the total flavonoid content (TFC) in herbal drug and derived products from Bauhinia monandra Kurz. Several analytical parameters from this method grounded on the complex formed between flavonoids and AlCl3 were evaluated such as herbal amount (0.25 to 1.25 g); solvent composition (ethanol 40 to 80%, v/v); as well as the reaction time and AlCl3 concentration (2 to 9%, w/v). The method was adjusted to aqueous extractives and its performance studied through precision, linearity and preliminary robustness. The results showed an important dependence of the method response from reaction time, AlCl3 concentration, sample amount, and solvent mixture. After choosing the optimized condition, the method was applied for the matrixes (herbal material and extractives), showing precision lower than 5% (for both parameters repeatability and intermediate precision), coefficient of determination higher than 0.99, and no important influence could be observed for slight variations from wavelength or AlCl3 concentration. Thus, it could be concluded that the evaluated analytical procedure was suitable to quantify the total flavonoid content in raw material and aqueous extractives from leaves of B. monandra.http://dx.doi.org/10.1100/2012/923462 |
| spellingShingle | Ana Josane Dantas Fernandes Magda Rhayanny Assunção Ferreira Karina Perrelli Randau Tatiane Pereira de Souza Luiz Alberto Lira Soares Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) The Scientific World Journal |
| title | Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) |
| title_full | Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) |
| title_fullStr | Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) |
| title_full_unstemmed | Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) |
| title_short | Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae) |
| title_sort | total flavonoids content in the raw material and aqueous extractives from bauhinia monandra kurz caesalpiniaceae |
| url | http://dx.doi.org/10.1100/2012/923462 |
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