Controlling Oral Polymicrobial Biofilm Using Usnic Acid on the Surface of Titanium in the Artificial Saliva Media
<b>Background/Objectives:</b> Titanium dental implants, while highly successful, face challenges due to polymicrobial infections leading to peri-implantitis and implant failure. Biofilm formation on implant surfaces is the primary cause of these infections, with factors such as matrix pr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/14/2/115 |
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| Summary: | <b>Background/Objectives:</b> Titanium dental implants, while highly successful, face challenges due to polymicrobial infections leading to peri-implantitis and implant failure. Biofilm formation on implant surfaces is the primary cause of these infections, with factors such as matrix production and cross-kingdom interactions contributing to the microbial accumulation of bacterial and fungal pathogens species. To combat this issue, naturally derived molecules have been reported to overcome the hurdle of antimicrobial resistance against the application of conventional antibiotics and antifungals. <b>Methods:</b> The present study aimed to employ the lichen-derived molecules, usnic acid (UA), to retard the development of biofilms of bacterial and fungal pathogens on the surface of titanium kept in the human artificial saliva (HAS) working as a growth-supporting, host-mimicking media. <b>Results:</b> The minimum inhibitory concentration of UA in HAS towards <i>Candida albicans</i> was >512 µg/mL, whereas against <i>Staphylococcus aureus</i> and <i>Streptococcus mutans</i>, it was determined to be 512 µg/mL. Whereas, in the standard growth media, the MIC value of UA towards <i>S. mutans</i> and <i>S. aureus</i> were 8 and 16 µg/mL; however, against <i>C. albicans</i>, it was 512 µg/mL. UA synergistically enhanced the efficacy of the antibiotics toward bacterial pathogens and the efficacy of antifungals against <i>C. albicans</i>. The antibiofilm results depict the fact that in the HAS, UA significantly reduced both mono-species of <i>S. mutans</i>, <i>S. aureus</i>, and <i>C. albicans</i> and mixed-species biofilm of <i>C. albicans</i> with <i>S. mutans</i> and <i>S. aureus</i> on the surface of the titanium. <b>Conclusions:</b> The present study showed that UA is a promising natural drug that can control oral polymicrobial disease as a result of the application of dental implants. |
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| ISSN: | 2079-6382 |