Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress
The kinesin (<i>KIN</i>) gene family is a subgroup of motor proteins. It plays a critical role in plant development and responses to environmental stresses. However, their function in soybean salt tolerance has yet to be clearly defined. This study employed bioinformatics approaches and...
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MDPI AG
2025-01-01
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| author | Ting Jin Kai Zhang Xiujie Zhang Chunhua Wu Weihua Long |
| author_facet | Ting Jin Kai Zhang Xiujie Zhang Chunhua Wu Weihua Long |
| author_sort | Ting Jin |
| collection | DOAJ |
| description | The kinesin (<i>KIN</i>) gene family is a subgroup of motor proteins. It plays a critical role in plant development and responses to environmental stresses. However, their function in soybean salt tolerance has yet to be clearly defined. This study employed bioinformatics approaches and identified 139 kinesin family members in the soybean genome. These 139 genes were classified into 10 subgroups, unevenly distributed across the chromosomes. The promoter regions of <i>GmKIN</i> genes harbored several stress-responsive elements, and segmental duplication was the primary driver of the expansion of the <i>GmKIN</i> gene family. Based on publicly available RNA-seq data, we studied the response patterns of 139 <i>GmKIN</i> genes to salt stress and found that 20 <i>KIN</i> genes in soybeans were upregulated after salt stress, with <i>GmKIN114</i>, <i>GmKIN102</i>, <i>GmKIN109</i>, and <i>GmKIN99</i> showing more than a threefold increase in their expression under salt stress. Using quantitative fluorescence PCR, transgenic yeast, and a transgenic hairy root system, we preliminarily validated the salt tolerance functions of the four <i>KIN</i> genes in soybeans. This study probed into the <i>GmKIN</i> gene family in soybean, offering valuable insights into the functional roles of these genes in stress adaptation. |
| format | Article |
| id | doaj-art-eaf423d566a94758af9fcc78ef68f928 |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-eaf423d566a94758af9fcc78ef68f9282025-08-20T02:44:43ZengMDPI AGAgronomy2073-43952025-01-0115227510.3390/agronomy15020275Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt StressTing Jin0Kai Zhang1Xiujie Zhang2Chunhua Wu3Weihua Long4College of Rural Revitalization, Jiangsu Open University, Nanjing 210036, ChinaCollege of Agronomy, Nanjing Agricultural University, Nanjing 211800, ChinaCollege of Agronomy, Nanjing Agricultural University, Nanjing 211800, ChinaCollege of Agronomy, Nanjing Agricultural University, Nanjing 211800, ChinaCollege of Rural Revitalization, Jiangsu Open University, Nanjing 210036, ChinaThe kinesin (<i>KIN</i>) gene family is a subgroup of motor proteins. It plays a critical role in plant development and responses to environmental stresses. However, their function in soybean salt tolerance has yet to be clearly defined. This study employed bioinformatics approaches and identified 139 kinesin family members in the soybean genome. These 139 genes were classified into 10 subgroups, unevenly distributed across the chromosomes. The promoter regions of <i>GmKIN</i> genes harbored several stress-responsive elements, and segmental duplication was the primary driver of the expansion of the <i>GmKIN</i> gene family. Based on publicly available RNA-seq data, we studied the response patterns of 139 <i>GmKIN</i> genes to salt stress and found that 20 <i>KIN</i> genes in soybeans were upregulated after salt stress, with <i>GmKIN114</i>, <i>GmKIN102</i>, <i>GmKIN109</i>, and <i>GmKIN99</i> showing more than a threefold increase in their expression under salt stress. Using quantitative fluorescence PCR, transgenic yeast, and a transgenic hairy root system, we preliminarily validated the salt tolerance functions of the four <i>KIN</i> genes in soybeans. This study probed into the <i>GmKIN</i> gene family in soybean, offering valuable insights into the functional roles of these genes in stress adaptation.https://www.mdpi.com/2073-4395/15/2/275kinesin genegene familysalt stresssoybeanhairy roots |
| spellingShingle | Ting Jin Kai Zhang Xiujie Zhang Chunhua Wu Weihua Long Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress Agronomy kinesin gene gene family salt stress soybean hairy roots |
| title | Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress |
| title_full | Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress |
| title_fullStr | Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress |
| title_full_unstemmed | Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress |
| title_short | Genome-Wide Identification of the Kinesin Gene Family in Soybean and Its Response to Salt Stress |
| title_sort | genome wide identification of the kinesin gene family in soybean and its response to salt stress |
| topic | kinesin gene gene family salt stress soybean hairy roots |
| url | https://www.mdpi.com/2073-4395/15/2/275 |
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