Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid

Gallic acid (GA) is widely used in beverages, food, and other fields as antioxidant. However, GA is slightly toxic and the accumulation of GA is harmful to human body. Therefore, it's vital to develop simple and sensitive detection methods for GA. In this work, a novel ratiometric fluorescent n...

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Main Authors: Chunlei Yang, Guiju Xu, Chenghao Hou, Hongwei Zhang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157524007314
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author Chunlei Yang
Guiju Xu
Chenghao Hou
Hongwei Zhang
author_facet Chunlei Yang
Guiju Xu
Chenghao Hou
Hongwei Zhang
author_sort Chunlei Yang
collection DOAJ
description Gallic acid (GA) is widely used in beverages, food, and other fields as antioxidant. However, GA is slightly toxic and the accumulation of GA is harmful to human body. Therefore, it's vital to develop simple and sensitive detection methods for GA. In this work, a novel ratiometric fluorescent nanoprobe (named CoOOH/OPD/SiNPs) for the GA detection in different foods was designed and prepared. The fluorescence of silicon nanoparticles (SiNPs) at 443 nm would be quenched by cobalt oxyhydroxide (CoOOH) nanoflakes. o-phenylenediamine (OPD) would be oxidized to 2,3-diaminophenazine (DAP) by CoOOH nanoflakes that have peroxidase-like activity, which produces a new fluorescent peak at 556 nm. Meanwhile, SiNPs' fluorescence would be quenched through DAP due to inner filter effect (IFE). With the addition of GA, the reductive decomposition of CoOOH decreased DAP level, causing IFE being restrained. The concentration of GA indicates an excellent linear relationship with fluorescence ratio (F443/F556) in range of 0.4–12 μM (R2 = 0.9937) with 0.16 μM detection limit. This nanoprobe is applied to GA detection in water, tea leaves, fruits and nut fruits, which would be expected to act as a portable device for complex substances analysis.
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publishDate 2024-12-01
publisher Elsevier
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series Food Chemistry: X
spelling doaj-art-eaf189d80f7b4903b34b3ffa4bba58cb2025-08-20T01:59:34ZengElsevierFood Chemistry: X2590-15752024-12-012410184310.1016/j.fochx.2024.101843Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acidChunlei Yang0Guiju Xu1Chenghao Hou2Hongwei Zhang3Corresponding authors.; Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, PR ChinaInstitute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, PR ChinaInstitute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, PR ChinaCorresponding authors.; Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, PR ChinaGallic acid (GA) is widely used in beverages, food, and other fields as antioxidant. However, GA is slightly toxic and the accumulation of GA is harmful to human body. Therefore, it's vital to develop simple and sensitive detection methods for GA. In this work, a novel ratiometric fluorescent nanoprobe (named CoOOH/OPD/SiNPs) for the GA detection in different foods was designed and prepared. The fluorescence of silicon nanoparticles (SiNPs) at 443 nm would be quenched by cobalt oxyhydroxide (CoOOH) nanoflakes. o-phenylenediamine (OPD) would be oxidized to 2,3-diaminophenazine (DAP) by CoOOH nanoflakes that have peroxidase-like activity, which produces a new fluorescent peak at 556 nm. Meanwhile, SiNPs' fluorescence would be quenched through DAP due to inner filter effect (IFE). With the addition of GA, the reductive decomposition of CoOOH decreased DAP level, causing IFE being restrained. The concentration of GA indicates an excellent linear relationship with fluorescence ratio (F443/F556) in range of 0.4–12 μM (R2 = 0.9937) with 0.16 μM detection limit. This nanoprobe is applied to GA detection in water, tea leaves, fruits and nut fruits, which would be expected to act as a portable device for complex substances analysis.http://www.sciencedirect.com/science/article/pii/S2590157524007314CoOOH nanoflakesRatiometric fluorescenceGallic acid detectionFluorescent silicon nanoparticles
spellingShingle Chunlei Yang
Guiju Xu
Chenghao Hou
Hongwei Zhang
Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
Food Chemistry: X
CoOOH nanoflakes
Ratiometric fluorescence
Gallic acid detection
Fluorescent silicon nanoparticles
title Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
title_full Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
title_fullStr Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
title_full_unstemmed Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
title_short Cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
title_sort cobalt oxyhydroxide nanoflakes enable ratiometric fluorescent assay of gallic acid
topic CoOOH nanoflakes
Ratiometric fluorescence
Gallic acid detection
Fluorescent silicon nanoparticles
url http://www.sciencedirect.com/science/article/pii/S2590157524007314
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AT guijuxu cobaltoxyhydroxidenanoflakesenableratiometricfluorescentassayofgallicacid
AT chenghaohou cobaltoxyhydroxidenanoflakesenableratiometricfluorescentassayofgallicacid
AT hongweizhang cobaltoxyhydroxidenanoflakesenableratiometricfluorescentassayofgallicacid