Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques
Summary: A SARS-CoV-2 spike DNA vaccine formulated with a cationic nanoparticle emulsion (LION) was tested in Rhesus macaques. It induced robust, long-lasting (>2 years) cellular and humoral immunity, including increased neutralization breadth. T cell responses were predominantly CD8+, in contras...
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Elsevier
2025-04-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225004936 |
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| author | Sevasti Karaliota Maha Moussa Margherita Rosati Santhi Devasundaram Soumya Sengupta Katherine C. Goldfarbmuren Robert Burns Jenifer Bear Dimitris Stellas Elizabeth A. Urban Claire Deleage Amit P. Khandhar Jesse Erasmus Peter Berglund Steven G. Reed George N. Pavlakis Barbara K. Felber |
| author_facet | Sevasti Karaliota Maha Moussa Margherita Rosati Santhi Devasundaram Soumya Sengupta Katherine C. Goldfarbmuren Robert Burns Jenifer Bear Dimitris Stellas Elizabeth A. Urban Claire Deleage Amit P. Khandhar Jesse Erasmus Peter Berglund Steven G. Reed George N. Pavlakis Barbara K. Felber |
| author_sort | Sevasti Karaliota |
| collection | DOAJ |
| description | Summary: A SARS-CoV-2 spike DNA vaccine formulated with a cationic nanoparticle emulsion (LION) was tested in Rhesus macaques. It induced robust, long-lasting (>2 years) cellular and humoral immunity, including increased neutralization breadth. T cell responses were predominantly CD8+, in contrast to other DNA vaccines. A rapid transient cytokine/chemokine response was associated with expansion and trafficking of myeloid cells and lymphocytes. Increased proliferation and dynamic changes between blood and lymph node (LN) were found for monocyte-derived cells, dendritic cells, and B and T cells, resulting in activation of LN and expansion of germinal centers (GCs), likely critical in shaping long-lasting adaptive immunity. Significant GC expansion of B, CD4−, and CD8− cells, including the Tfc3 subset, reflects a balanced immune response, including antibody (Ab) development. DNA/LION vaccination activates myeloid and lymphoid cells in blood and LN and promotes effective antigen presentation, resulting in sustained antigen-specific cellular and humoral responses, emerging as an effective DNA vaccine delivery platform. |
| format | Article |
| id | doaj-art-eaebaf88db0e45e8b06d10e9c352147f |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-eaebaf88db0e45e8b06d10e9c352147f2025-08-20T02:10:20ZengElsevieriScience2589-00422025-04-0128411223210.1016/j.isci.2025.112232Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaquesSevasti Karaliota0Maha Moussa1Margherita Rosati2Santhi Devasundaram3Soumya Sengupta4Katherine C. Goldfarbmuren5Robert Burns6Jenifer Bear7Dimitris Stellas8Elizabeth A. Urban9Claire Deleage10Amit P. Khandhar11Jesse Erasmus12Peter Berglund13Steven G. Reed14George N. Pavlakis15Barbara K. Felber16Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA; Basic Science Program, Leidos Biomedical Research, Inc., Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAAdvanced Biomedical Computational Science, Leidos Biomedical Research, Inc., Frederick, MD, USA; Vaccine Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USAAIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USAAIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USAHDT Bio Corp, Seattle, WA, USAHDT Bio Corp, Seattle, WA, USAHDT Bio Corp, Seattle, WA, USAHDT Bio Corp, Seattle, WA, USACenter for Cancer Research, National Cancer Institute, Frederick, MD, USAHuman Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA; Corresponding authorSummary: A SARS-CoV-2 spike DNA vaccine formulated with a cationic nanoparticle emulsion (LION) was tested in Rhesus macaques. It induced robust, long-lasting (>2 years) cellular and humoral immunity, including increased neutralization breadth. T cell responses were predominantly CD8+, in contrast to other DNA vaccines. A rapid transient cytokine/chemokine response was associated with expansion and trafficking of myeloid cells and lymphocytes. Increased proliferation and dynamic changes between blood and lymph node (LN) were found for monocyte-derived cells, dendritic cells, and B and T cells, resulting in activation of LN and expansion of germinal centers (GCs), likely critical in shaping long-lasting adaptive immunity. Significant GC expansion of B, CD4−, and CD8− cells, including the Tfc3 subset, reflects a balanced immune response, including antibody (Ab) development. DNA/LION vaccination activates myeloid and lymphoid cells in blood and LN and promotes effective antigen presentation, resulting in sustained antigen-specific cellular and humoral responses, emerging as an effective DNA vaccine delivery platform.http://www.sciencedirect.com/science/article/pii/S2589004225004936Natural sciencesBiological sciencesImmunologyImmune responseBiotechnologyNanotechnology |
| spellingShingle | Sevasti Karaliota Maha Moussa Margherita Rosati Santhi Devasundaram Soumya Sengupta Katherine C. Goldfarbmuren Robert Burns Jenifer Bear Dimitris Stellas Elizabeth A. Urban Claire Deleage Amit P. Khandhar Jesse Erasmus Peter Berglund Steven G. Reed George N. Pavlakis Barbara K. Felber Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques iScience Natural sciences Biological sciences Immunology Immune response Biotechnology Nanotechnology |
| title | Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques |
| title_full | Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques |
| title_fullStr | Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques |
| title_full_unstemmed | Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques |
| title_short | Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques |
| title_sort | highly immunogenic dna lion nanocarrier vaccine potently activates lymph nodes inducing long lasting immunity in macaques |
| topic | Natural sciences Biological sciences Immunology Immune response Biotechnology Nanotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225004936 |
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