A bivalent mRNA vaccine against RSV infection in rodent models
Because of the higher conservation of RSV Fusion (F) protein than the glycoprotein (G) across RSV strains and serotypes, the majority of vaccine candidates targets to viral fusion protein (F) rather than glycoprotein to elicit a broader range of protective neutralizing antibodies from infection. In...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-03-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1542592/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849391970633383936 |
|---|---|
| author | Juan Liu Hanqing Zhao Wenhao Wang Binbin Yang Naifang Zhang Yu Zhang Jie Qian Qiaofang Ma Yankun Lu Huafeng Han Yongsheng Yang |
| author_facet | Juan Liu Hanqing Zhao Wenhao Wang Binbin Yang Naifang Zhang Yu Zhang Jie Qian Qiaofang Ma Yankun Lu Huafeng Han Yongsheng Yang |
| author_sort | Juan Liu |
| collection | DOAJ |
| description | Because of the higher conservation of RSV Fusion (F) protein than the glycoprotein (G) across RSV strains and serotypes, the majority of vaccine candidates targets to viral fusion protein (F) rather than glycoprotein to elicit a broader range of protective neutralizing antibodies from infection. In this study, we screened two chemically modified mRNA vaccines expressing RSV prefusion stabilized protein (preF) targeting RSV A2 and B subtypes. After immunization, the antigen-specific binding antibody, neutralizing antibody, and T cell-mediated immune response were evaluated. After challenge with live RSV A2 virus in cotton rats, the protection and safety of vaccine was further evaluated. The results showed that the mRNA vaccine candidates elicited robust antigen-specific binding antibody, neutralizing antibody responses and Th1-biased T-cell responses in both mice and cotton rats. Moreover, cotton rats vaccinated with mRNA vaccine, lung pathology and lung infectious viral loads were significantly reduced, and no vaccine enhanced respiratory disease (VERD) happened. These results collectively demonstrated that mRNA-based vaccine induced strong humoral and cellular immunity, provided outstanding protection against both RSV A2 and RSV B subtypes in rodent animals as well. Our data demonstrated that these mRNA vaccines should be further evaluated in clinical trials. |
| format | Article |
| id | doaj-art-eae96afc4c0d4ab9bbcfb928fe1f1554 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-eae96afc4c0d4ab9bbcfb928fe1f15542025-08-20T03:40:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-03-011610.3389/fimmu.2025.15425921542592A bivalent mRNA vaccine against RSV infection in rodent modelsJuan LiuHanqing ZhaoWenhao WangBinbin YangNaifang ZhangYu ZhangJie QianQiaofang MaYankun LuHuafeng HanYongsheng YangBecause of the higher conservation of RSV Fusion (F) protein than the glycoprotein (G) across RSV strains and serotypes, the majority of vaccine candidates targets to viral fusion protein (F) rather than glycoprotein to elicit a broader range of protective neutralizing antibodies from infection. In this study, we screened two chemically modified mRNA vaccines expressing RSV prefusion stabilized protein (preF) targeting RSV A2 and B subtypes. After immunization, the antigen-specific binding antibody, neutralizing antibody, and T cell-mediated immune response were evaluated. After challenge with live RSV A2 virus in cotton rats, the protection and safety of vaccine was further evaluated. The results showed that the mRNA vaccine candidates elicited robust antigen-specific binding antibody, neutralizing antibody responses and Th1-biased T-cell responses in both mice and cotton rats. Moreover, cotton rats vaccinated with mRNA vaccine, lung pathology and lung infectious viral loads were significantly reduced, and no vaccine enhanced respiratory disease (VERD) happened. These results collectively demonstrated that mRNA-based vaccine induced strong humoral and cellular immunity, provided outstanding protection against both RSV A2 and RSV B subtypes in rodent animals as well. Our data demonstrated that these mRNA vaccines should be further evaluated in clinical trials.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1542592/fullRSV prefusion proteinmRNA vaccinesneutralizing antibodyprotectionimmunity |
| spellingShingle | Juan Liu Hanqing Zhao Wenhao Wang Binbin Yang Naifang Zhang Yu Zhang Jie Qian Qiaofang Ma Yankun Lu Huafeng Han Yongsheng Yang A bivalent mRNA vaccine against RSV infection in rodent models Frontiers in Immunology RSV prefusion protein mRNA vaccines neutralizing antibody protection immunity |
| title | A bivalent mRNA vaccine against RSV infection in rodent models |
| title_full | A bivalent mRNA vaccine against RSV infection in rodent models |
| title_fullStr | A bivalent mRNA vaccine against RSV infection in rodent models |
| title_full_unstemmed | A bivalent mRNA vaccine against RSV infection in rodent models |
| title_short | A bivalent mRNA vaccine against RSV infection in rodent models |
| title_sort | bivalent mrna vaccine against rsv infection in rodent models |
| topic | RSV prefusion protein mRNA vaccines neutralizing antibody protection immunity |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1542592/full |
| work_keys_str_mv | AT juanliu abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT hanqingzhao abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT wenhaowang abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT binbinyang abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT naifangzhang abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yuzhang abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT jieqian abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT qiaofangma abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yankunlu abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT huafenghan abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yongshengyang abivalentmrnavaccineagainstrsvinfectioninrodentmodels AT juanliu bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT hanqingzhao bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT wenhaowang bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT binbinyang bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT naifangzhang bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yuzhang bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT jieqian bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT qiaofangma bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yankunlu bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT huafenghan bivalentmrnavaccineagainstrsvinfectioninrodentmodels AT yongshengyang bivalentmrnavaccineagainstrsvinfectioninrodentmodels |