Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer

Abstract Natural polymers such as pectin have garnered significant interest in recent years due to their potential applications in gastroretentive drug delivery systems. Gastroretentive dosage forms are intended to extend the emptying time of drugs in the gastrointestinal tract. Accordingly, this st...

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Main Authors: Ebrahim Abdela Siraj, Yohannes Mulualem, Fantahun Molla, Ashagrachew Tewabe Yayehrad, Anteneh Belete
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-95732-1
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author Ebrahim Abdela Siraj
Yohannes Mulualem
Fantahun Molla
Ashagrachew Tewabe Yayehrad
Anteneh Belete
author_facet Ebrahim Abdela Siraj
Yohannes Mulualem
Fantahun Molla
Ashagrachew Tewabe Yayehrad
Anteneh Belete
author_sort Ebrahim Abdela Siraj
collection DOAJ
description Abstract Natural polymers such as pectin have garnered significant interest in recent years due to their potential applications in gastroretentive drug delivery systems. Gastroretentive dosage forms are intended to extend the emptying time of drugs in the gastrointestinal tract. Accordingly, this study was aimed to examine the use of pectin as a floating and mucoadhesive polymer using furosemide as a model drug. The citrus peel pectin was extracted from local Citrus aurantifolia tree fruit peel using hot water extraction technique. FTIR and DSC analyses were performed to investigate the compatibility of the pectin extracted with furosemide. C. aurantifolia peel yielded 34.4% (w/w) purified pectin with a degree of esterification of 85.49%. FTIR and DSC analysis revealed that the citrus peel pectin powder is compatible with furosemide. Based on preliminary study, pectin and effervescent agent concentration were identified as significant independent variables. Hence, their influence on five response variables (floating duration, bioadhesive strength, swelling index, drug release at 1 h, and drug release rate) were further studied and optimized using central composite design (CCD). Accordingly, the CCD model predicted an optimum formulation at 22.3% of pectin concentration and 5% of effervescent agent. Under this condition, the Design-Expert software predicted floating duration (14.07 s), bioadhesive strength (28.57 g), swelling index (254.08%), drug release at 1 h (27.86%), and drug release rate (28.045%/h−1/2). The validity of the predicted optimum formulation was confirmed experimentally. The optimized tablet formulation showed pharmacopeial acceptable characteristics. In conclusion, the results of this study suggest that waste-derived citrus pectin can be considered as an abundant alternative pharmaceutical excipient in the formulation and manufacture of floating and bioadhesive matrix tablets with favorable floating and bioadhesion characteristics.
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spelling doaj-art-eae83fa6c7414a2db3dead53871bed462025-08-20T03:10:17ZengNature PortfolioScientific Reports2045-23222025-05-0115111610.1038/s41598-025-95732-1Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymerEbrahim Abdela Siraj0Yohannes Mulualem1Fantahun Molla2Ashagrachew Tewabe Yayehrad3Anteneh Belete4Department of Pharmaceutics and Social Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa UniversityDepartment of Pharmaceutics and Social Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa UniversityDepartment of Pharmaceutics and Social Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa UniversityDepartment of Pharmacy, College of Medicine and Health Sciences, Bahir Dar UniversityDepartment of Pharmaceutics and Social Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa UniversityAbstract Natural polymers such as pectin have garnered significant interest in recent years due to their potential applications in gastroretentive drug delivery systems. Gastroretentive dosage forms are intended to extend the emptying time of drugs in the gastrointestinal tract. Accordingly, this study was aimed to examine the use of pectin as a floating and mucoadhesive polymer using furosemide as a model drug. The citrus peel pectin was extracted from local Citrus aurantifolia tree fruit peel using hot water extraction technique. FTIR and DSC analyses were performed to investigate the compatibility of the pectin extracted with furosemide. C. aurantifolia peel yielded 34.4% (w/w) purified pectin with a degree of esterification of 85.49%. FTIR and DSC analysis revealed that the citrus peel pectin powder is compatible with furosemide. Based on preliminary study, pectin and effervescent agent concentration were identified as significant independent variables. Hence, their influence on five response variables (floating duration, bioadhesive strength, swelling index, drug release at 1 h, and drug release rate) were further studied and optimized using central composite design (CCD). Accordingly, the CCD model predicted an optimum formulation at 22.3% of pectin concentration and 5% of effervescent agent. Under this condition, the Design-Expert software predicted floating duration (14.07 s), bioadhesive strength (28.57 g), swelling index (254.08%), drug release at 1 h (27.86%), and drug release rate (28.045%/h−1/2). The validity of the predicted optimum formulation was confirmed experimentally. The optimized tablet formulation showed pharmacopeial acceptable characteristics. In conclusion, the results of this study suggest that waste-derived citrus pectin can be considered as an abundant alternative pharmaceutical excipient in the formulation and manufacture of floating and bioadhesive matrix tablets with favorable floating and bioadhesion characteristics.https://doi.org/10.1038/s41598-025-95732-1Citrus aurantifoliaFloatingBioadhesivePectinOptimizationFurosemide
spellingShingle Ebrahim Abdela Siraj
Yohannes Mulualem
Fantahun Molla
Ashagrachew Tewabe Yayehrad
Anteneh Belete
Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
Scientific Reports
Citrus aurantifolia
Floating
Bioadhesive
Pectin
Optimization
Furosemide
title Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
title_full Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
title_fullStr Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
title_full_unstemmed Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
title_short Formulation optimization of furosemide floating-bioadhesive matrix tablets using waste-derived Citrus aurantifolia peel pectin as a polymer
title_sort formulation optimization of furosemide floating bioadhesive matrix tablets using waste derived citrus aurantifolia peel pectin as a polymer
topic Citrus aurantifolia
Floating
Bioadhesive
Pectin
Optimization
Furosemide
url https://doi.org/10.1038/s41598-025-95732-1
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