EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS
Human platelet lysates (HPL) have been described as appropriate cell culturesupplement for cultures of mesenchymal stem cells (MSCs). The increase in demandfor harmless and animal-free cultures of MSCs is related to the potential applicationof MSCs grow in human source supplementsIn this study, huma...
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College of Veterinary Medicine, University of Basrah, Iraq
2016-08-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_174889_d3206e90d46e36a5614c4c877e8cb5ef.pdf |
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| _version_ | 1850137441435910144 |
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| author | Majid Hameed Mohammed |
| author_facet | Majid Hameed Mohammed |
| author_sort | Majid Hameed Mohammed |
| collection | DOAJ |
| description | Human platelet lysates (HPL) have been described as appropriate cell culturesupplement for cultures of mesenchymal stem cells (MSCs). The increase in demandfor harmless and animal-free cultures of MSCs is related to the potential applicationof MSCs grow in human source supplementsIn this study, human platelet lysates are derived from fresh and expired blooddonor platelet concentrates. Mouse bone marrow-derived mesenchymal stem cellswere cultured with one of three culture supplements; fetal bovine serum, lysates fromfresh and expired prepared human platelet concentrates. The impacts of these plateletderived culture supplements on basic mesenchymal stem cell characteristics wereevaluated. All cultures preserved the mesenchymal stem cells surface markerexpression, growth carves and viability test. The result demonstrated thatmesenchymal stem cells complemented with platelet lysates proliferated faster thanconventional FBS cultured cells and increased the expression of the cells. Inconclusion, the use of fresh and expired platelet units from blood banks to prepareplatelet lysates for the culture of MSCs is appropriate as well as keep cells phenotypecharacteristics and also shortens culture time by increasing their growth. |
| format | Article |
| id | doaj-art-eae5710c33d942a6a6c5a361cddc1452 |
| institution | OA Journals |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-eae5710c33d942a6a6c5a361cddc14522025-08-20T02:30:50ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562016-08-0115413214310.23975/bjvetr.2016.174889174889EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLSMajid Hameed Mohammed0Faculty os science, Center of Scientific Research,University of Dohouk,Kurdistan,IraqHuman platelet lysates (HPL) have been described as appropriate cell culturesupplement for cultures of mesenchymal stem cells (MSCs). The increase in demandfor harmless and animal-free cultures of MSCs is related to the potential applicationof MSCs grow in human source supplementsIn this study, human platelet lysates are derived from fresh and expired blooddonor platelet concentrates. Mouse bone marrow-derived mesenchymal stem cellswere cultured with one of three culture supplements; fetal bovine serum, lysates fromfresh and expired prepared human platelet concentrates. The impacts of these plateletderived culture supplements on basic mesenchymal stem cell characteristics wereevaluated. All cultures preserved the mesenchymal stem cells surface markerexpression, growth carves and viability test. The result demonstrated thatmesenchymal stem cells complemented with platelet lysates proliferated faster thanconventional FBS cultured cells and increased the expression of the cells. Inconclusion, the use of fresh and expired platelet units from blood banks to prepareplatelet lysates for the culture of MSCs is appropriate as well as keep cells phenotypecharacteristics and also shortens culture time by increasing their growth.https://bjvr.uobasrah.edu.iq/article_174889_d3206e90d46e36a5614c4c877e8cb5ef.pdfstromal cellsplatelet lysatelysate |
| spellingShingle | Majid Hameed Mohammed EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS Basrah Journal of Veterinary Research stromal cells platelet lysate lysate |
| title | EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS |
| title_full | EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS |
| title_fullStr | EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS |
| title_full_unstemmed | EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS |
| title_short | EFFECTS OF HUMAN PLATELET LYSATE PREPARATIONS ON THE PROLIFERATION OF BONE MARROW DERIVED MICE MESENCHYMAL STEM CELLS |
| title_sort | effects of human platelet lysate preparations on the proliferation of bone marrow derived mice mesenchymal stem cells |
| topic | stromal cells platelet lysate lysate |
| url | https://bjvr.uobasrah.edu.iq/article_174889_d3206e90d46e36a5614c4c877e8cb5ef.pdf |
| work_keys_str_mv | AT majidhameedmohammed effectsofhumanplateletlysatepreparationsontheproliferationofbonemarrowderivedmicemesenchymalstemcells |